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mature miRNA: TGCACTGCCTCTTCCCTGGCT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52387152|gb|CV232037.1|CV232037 WS0196.B21_F11 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS0196_F11 3', mRNA sequence

TGCACTGCCTCTTCCCTGGCT
||||||||||||| |||||.
CCGTGACGGAGAAGTGACCGG

EST sequence (targeted fragment marked with yellow):
CCTAATTCTTGTGCTTGCCGCTCCAGCAGCTTATGCTGCTACAACGTACACTGTTGGTGACTCGTCCGGCTGGACCACGTTAGGAGATTACACCACTTGGGTTAGTGGTAAAACATTCACTGTTGGTGACTCTCTTCTGTTCAAATATAGCTCCACACACAGTGTGGCTGAAGTATCGAAAGACGACTACGATAGTTGCAGCACAAGTAATCTCGGGAAAACCTATACTGATGGAAGCTCAACAGTACTCTTGTCCACGGCTGGTCCAATGTACTTCATTTGTTCCACACCTGGTCACTGTTCCGGCGGCATGAAGCTAGCAATCACCGTTGCCGCAGCCAGTGGCACCCCAAGTACCCCCACCACACCTGCCCCTCCAGTCGACGATGGTTCAACCACTCCATCAACTACATCTGGCTCGTCACCTACAGCCCCCACCGGCACACCACCACCACCGTCGAAGAGTGATAATGGAGCCACCAGCATTTTATATAACATGATGCTTGGTGTCTTTCTTGTATTCGGAACAACTGTTGCATTGATGGGCCAGTGAAGAGGCAGTGCCATATTATCGTGTTGTTAATTAATGTCCTTGTTATCATTGGGTTGAAGCTGGTTTTTTTCAATTTTTATTGGATGAGGGATGTGAAATTTATGTTTATATATATTATGTTATTGAATAATTAATTTTTATTTATAAGCTCCAAAAAAAAAAAAAAAAAA



gi|24059100|gb|BU868453.1|BU868453 M116A08 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

TGCACTGCCTCTTCCCTGGCT
||||||||||| |||||||
TCGTGACGGAGATGGGACCGT

EST sequence (targeted fragment marked with yellow):
GGAACCCAAATACCTTCTTCTCTTCTCTTCTTTTCTCCCGGAAAATGGCCGGTTTAATTTCAAGATCAGTTCCTTGTGCAATCCTAGTAGTCTTGTGCACGTGGTGCCCATTTTGGCTAAAGATCACACTGTAGGAGATAGTTCAGGCTGGGCAATTGGTATGGATTATAGCACCTGGACTAGTGGCAAGACCTTTTCAGTTGGCGACAGCCTTGTGTTTAACTACGGAGGAGGCCACACGGTAGATGAAGTGAGAGCCAGTGACTACAGCACATGCACTACAGGCAATGCAATCACTTCAGATAGCAGTGGTGCTACCACAATAGCCCTCAAGACTGCCGGAACTCATTATTTCATTTGTGGTGTTCCTGGCCACTGTGGGAGTGGCATGAAGGTTGCAGTCACTGTTGCAGCAGCAGGATCGAGCACAAGTCCCTCCTCCGGAACTCCATCTTCTGATGGCACTACCACTTCTCCGGCCGGTAGTAACGTCACCAATTACAAGCCTTCATCCAACAACGTACCCGATTCATCCTTAGGGATCAATATTTCCCCATTTGTGGCTTTAGCCGGTACTTGTGTCGCCGTTTTTGTGATGGTTTTCTCATGAATTGCCAGGGTAGAGGCAGTGCTCTGCTAGATCCCTCTTTTTTTTTTTTTTTGGTTCTTGCTTTTCCTGAAGCTCTGCTTGTATTCAGGATTATTA