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mature miRNA: TGCACTGCTTCTTCCCTGGCT
species: Centaurea solstitialis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124665234|gb|EH759976.1|EH759976 CNSL6118.b1_L18.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL6118, 

TGCACTGCTTCTTCCCTGGCT
|||||..||||.|||||  ||
ACGTGGTGAAGGAGGGAAAGA

EST sequence (targeted fragment marked with yellow):
GCCCCTCACATTCATTCGTTTACTCCTCCCTTTTTGCTAAATTGCTCCACCTGCGCACGTTAGCAGAAATCCAAAACACACAGAAGAAGAAGAAGAAGAAAGGGAGGAAGTGGTGCATCTTAAACCTACAATCAATCGTTATTCCTTTTGTATATGGTCTAAGTGTGTGTATATATATATATACGCAAGTTTTTAGGGCTTTTTCTTCAACTCTATTTCGACTCTTCAATGGCCGCTCCACCGGCAAGGGCAAGAGTAGATTACGATTATCTCATTAAGCTCCTTTTGATCGGCGATAGCGGTGTGGGGAAGAGTTGCCTTCTTTTGCGTTTTTCAGATGGTTCTTTCACTACTAGTTTTATCACTACCATCGGTATCGATTTTAAGATCAGAACCATTGAACTTGATGGAAAACGGATCAAACTCCAGATCTGGGACACAGCTGGTCAGGAACGCTTCCGGACTATTACAACTGCTTATTACAGAGGAGCCATGGGTATTTTGTTGGTGTACGATGTTACAGACGAATCATCTTTTAATAACATAAGAAACTGGATTCGCAACATCGAGCAGCATGCCTCAGACAATGTTAACAAGATCCTGGTTGGAAACAAAGCCGACATGGATGAAAGCAAAAGGGCTGTGCCTACCGCANAGGGCCAAGCACTTGCGGATGAGTATGGGATTAAATTCTTTGAAACTAGTGCAAAAACAAATCTAAATGTGGAGCAAGTTTTCTTTTNCATCGCGAAGGATATCAAGCAACGGCTTTCGGAT


gi|124680199|gb|EH774939.1|EH774939 CNSM7251.b1_F14.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSM7251, 

TGCACTGCTTCTTCCCTGGCT
||||||||||||| | |.||.
ACGTGACGAAGAAAGAATCGG

EST sequence (targeted fragment marked with yellow):
TAGGCGGGGGTGATGAACTTTTAGTCGAATAAATTCAGAAAAAGGAAATTTGTGCACTTTTCCGGCGAACAATTCCGGCATCGGTTCAGAAGAACATCCCTCTGTTGTTGTCGATAAAATTAATCTTTATTTGATGTGAAACAAATTATTCTCAGGGTCTTGTGTGTTCAATGAGGAATAACATCGAGGGGAAGAGATTTTGGTGACTAGTAGGAATCCCATATTGAACATGGTTTACCACTCCAGAATTGTCGATTTGGGAGGAGTTGCTAAAGCTTGCGGGTGTCCCTTACTTCCGATTAGAAACCAAATGAAAGGACAACCCCGTTGCTCTGATCAAGGTGAAGATATTGTGGATGAAGCAATCAAGTTCTTTAGAGCAAATGTGTTCTTCAAACACTTTGATCTGAAAAGCCCTTCTGATAGAATTCTGGTTTATCTAACATATTATATAAATTTGGCCTTGAAAAGGCTTGAAGGATGCAGAACTGTGACTGAAGGAACAAATGCCATCAAGAACCTCAATTTGGAACATGTTTATGTTCCTGGTGAATCTGGTTTTCCTTTTCACAGAATCTTTCCCCGTCCCGGGGCTAAGAAAGAAGCAGTGCAGAGGCAATTCGGGATTATTTCAAGCAGATAAGGGAGGAAACGGGTAGAAGATTGTTGAACGTTGCATATAGAGCAAACGGGACTCCGAACAAATGGTGGTTGGCATTCGCCAAACGAAAGTACATGGCGTTCTAAAATGCCTGACCGAAGACTAATCAACCATTTGGTG