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mature miRNA: TGCAGTTGTTGTCTCAAGCTT
species: Saccharum officinarum
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|268777235|gb|CN608762.1|CN608762 SUL01-033F-B02-013.b Sugarcane leaf whorl library Saccharum hybrid cultivar CoS 767 cDNA clo 

TGCAGTTGTTGTCTCAAGCTT
|||||.|||||.|||| |||.
ACGTCGACAACGGAGTCCGAG

EST sequence (targeted fragment marked with yellow):
TGCTGCTAAGAAGGATGGGTCCTGTGACTGCAACTCTAGCTTTGAGTGTAATATCTGTCTTGACCCAGCTAAAGAGCCTGTGGTTACACCATGCGGTCACCTCTTCTGTTGGCCATGCTTGTACCAGTGGCTTCATGCACATTCACCCCACTCTGAGTGCCCTGTATGCAAGGGTGAGGTGCTAGAAGTGAATGTCACTCCGATATATGGAAGAGGCGGTGAAGAAGTGGATTCCACCAACCCTGACTTGCCACCCAGGCCACGAGCAAACAGAAGGGAGAGCCTGAGGCAACAGCTGCAAATGGCAGACACAAGAGGAAATGCATTGGTGAGGCAGTTGATACAAAACCATAGTATAGTTAGAGGCCTCCCAAGCCCAACAGGGATCGAAATGAATGTGGTTCCTGCAGGCCGGCAAAGGGCTAGGGCTCGGAGGCAGCAAAGGCAGGATAATA


gi|36067385|gb|CA297918.1|CA297918 SCCCSD1C02D04.g SD1 Saccharum hybrid cultivar (mixed) cDNA clone SCCCSD1C02D04 5', mRNA seque 

TGCAGTTGTTGTCTCAAGCTT
||||||||.|||||| |||||
ACGTCAACGACAGAGCTCGAA

EST sequence (targeted fragment marked with yellow):
GCTCTGCAGTGATATATATTATTCTTACATTGTCATTTGTCAGTATTATGATGATCCTCAAGTTTTAATAAAAAATTAAAATACACTATAAGTACGTTTATACCTTGCATAAGTTGATTTTACTAACGTTCAATTATTTTAATTAGATATGCATTTGGGGGCAGCAAGCTCGAGACAGCAACTGCATAACTTGCAAGAGCTTGTAAGTGTTCTTTTGTATTATGAGAAATATCATTTATCTTTTGTTATTCCCACAGCGATTGATAGATTGATACAGATTGGATCAATGTTTTTGGTTGGCCTAACTGGTTATTATATGTACTATGATGTGATAATTATACTAGGCTTGATTTTTTCGTAGAATACTACAAAGTACAATGAATGTGGTTAAAGGGTATAGTTGCAATGCCTGCTTACTTGGGTTCCACCCTAAGCAGGTCTCGTGCTTAGAGTACAAAGCATTTTATGTAAGACATAATCTGCTGCTGATCTCTCTCTCTTTGTTNGTGCACAGCAGATTTGGCAATAGGTCATTCTCGGCTGTTCTAGTTGTGTAGCAGAAGGGCAATAGGCCACCACTGGTACATTAGTTGGTAGCTAATACGCAAGCCATGTCTTGTGAGTGGCCTGAAGTAGAAATTTGACTGTTAGACTTAAGAGGATGATGGGCGTGTACTCACCCCGCGCAATGTGGCCCTTAATGGGGGCCAGAGGGGTGTTACTCTTTTACCTTGGGAGGAGAAAGGGGTTTTGTTCCCAACAACAAAAAATATCTTTGCACCGCTGGGTTGTGGGGGGGGCGCGGGACAACCCTTTT


gi|36055778|gb|CA294080.1|CA294080 SCSGLV1009F04.g LV1 Saccharum hybrid cultivar (mixed) cDNA clone SCSGLV1009F04 5', mRNA seque 

TGCAGTTGTTGTCTCAAGCTT
 |||| ||||| |||||||||
TCGTCCACAACCGAGTTCGAA

EST sequence (targeted fragment marked with yellow):
GANACGCGTGGGATGGGTGGCCTTCTCCATGTCACCCATATTATTCATGACCGTGTCGAAGATATCTCAACGGGGCTGCAAGCAGGAGATACCCTCAAGGTTATGATACTGAGCCATGACCGTGAAAGAGGCCGACTCAGCCTTTCTACAACGAAGCTTGAGCCAACACCTGCTGACATGATCCGTAATCCCAATCTTGTCTTTGAGAAGGCTGATGAGATGGCTCAGATATTCAGGCAGCGAATAGCTCAAGCAGAGGCAATGGCTCGTGCTGACATGTAGAGATTCCAGCCCGAGAGTGGATTGACCCTCACTTCAGAGGGCATCTTACGACCATAGTCGTCGGACGTACCTCCAGAGGATTCCGAACCCACAGATGAATAGAGGCAGGTGACGAACTGCACCGGATTTGACATATGTGATGGCAGTTCATCATACTTGTTTTTCTTCGACGGAACGGCCTGACCCAAGCATGTCCTGTTCAGATCGGTCTCCCCCTTTCAGTTATTCTCGTCTTCGGGTGTTGGCCATTTGAAAAGGTACGAAAGGTCTGAAGAACGCGAAAAGAACTGTATTCAGCGTACCTTGTGGAAAGCTCTTCGCATCTTGGCGGGACTCCCCAATTAAAAACAAAACTCCAGGGGCGCCCGCCC


gi|35996246|gb|CA276383.1|CA276383 SCEPSD2005F03.g SD2 Saccharum hybrid cultivar (mixed) cDNA clone SCEPSD2005F03 5', mRNA seque 

TGCAGTTGTTGTCTCAAGCTT
.||||| ||||||||  ||||
GCGTCACCAACAGAGAGCGAA

EST sequence (targeted fragment marked with yellow):
CCGACCATCGCCGAGGAACCAAAGCGAGAGACAACCACTGCGTGAACGAATCCATCGCCATGGCGTCTCGTCTCGTTGCGGCGGCGGCGGCGGCCTCCTCCTCCTCCTCCTCCGTGTCCCCCCTCGCCCGCCTAATCTCTCGCCGCGGCCTTGCAGGTGCCGCAGACGGCCATGGACCTGCGAAGGTGCCCTTGTGGAAAGATCCGTTGAGCCCGAGCAAGTGGAAGGAAGAGCACTTTGTTCTAGCAAGCCTCTCTATGTGGGGGGCTCTTATTTACGGTGGTTTCAAGCTTTTTGGTGGAAAGAAGGAAGACAAGACTGAGGTGGCACCAGCAAAGGCATAGTTGCTTGAGATGGAGCTCTACCTATTTCTGTGGCCTGGCAACAATGTTCTGTTAAATAAAAAGAAGTGTTCTATGTCGTGCTTTCAAGTTGATATAAATTGTGGCAAAGAGAGATTTTGATTTATGTAGGCAAGATTTTGAGAAGGTACACTTGCTCTCATCCACCATGAATAATCTTGCTGCANGGGAACTTCATGTTTGCGAACACTGCAAATGTTGTACTTCTGTCATGT