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mature miRNA: TGCCAAAGGAGAATTGCCCTG
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|9852079|gb|BE597006.1|BE597006 PI1_60_B12.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

TGCCAAAGGAGAATTGCCCTG
|||||||||||| |||||| .
ACGGTTTCCTCTAAACGGGCT

EST sequence (targeted fragment marked with yellow):
GCACGAGGTGGCGGCGGGTTGGAACTGCGACAGGGTCAGCTGATCTTGCCGGATAGACCACTCTATTGTGTTATCTCATTATAAGTGAAGCCCCCACTGATGAAAAGCTGTTATCTTGGTTGCTTTCTTGAGGAGTACTGGTCACCAGATGGTAGCATGTTCTCTTCTGTGTGATTGGGCTTGATTATGGACTGATGCTCCTGATCACTCTTGTCCTGTGATCATCCAGAGTATATCATCTGTCAGCTAGTCTTTCCTCTATTGGAATCACCTCCCTTTATGAAAGAATCCTTGTTATAGGGCAAATCTCCTTTGGCAAACATTGTTCAGGGTGCATCACTTTGTTGGATATCCTGAGCATATTCTCCTCGTCGATTGAACAGCAGCAAAGAGACTTCCAAATGATATCCGTTAGGAAATTCTCATTTAGTTTGATGGAAGTTTCATAAGGTTTCTTATCATCTGATTTGCTGGGCAAATCTCCTTTGGCAGTACTTATTAATAGCTCTTTTGGGCAAATCTCCTTTGGCATTAGCTGCTTCAGATATCTCTCTGGGCAAATCTCCTTTGGCATTAGTTTCTAGCTGTTTCATATTGCTCTTCGGGCAAATCTCCTTTGGCACTAAATACCATTTATATTTACTATTTTTGGTAAATCT



gi|9852079|gb|BE597006.1|BE597006 PI1_60_B12.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

TGCCAAAGGAGAATTGCCCTG
|||||||||||| |||||| .
ACGGTTTCCTCTAAACGGGTT

EST sequence (targeted fragment marked with yellow):
GCACGAGGTGGCGGCGGGTTGGAACTGCGACAGGGTCAGCTGATCTTGCCGGATAGACCACTCTATTGTGTTATCTCATTATAAGTGAAGCCCCCACTGATGAAAAGCTGTTATCTTGGTTGCTTTCTTGAGGAGTACTGGTCACCAGATGGTAGCATGTTCTCTTCTGTGTGATTGGGCTTGATTATGGACTGATGCTCCTGATCACTCTTGTCCTGTGATCATCCAGAGTATATCATCTGTCAGCTAGTCTTTCCTCTATTGGAATCACCTCCCTTTATGAAAGAATCCTTGTTATAGGGCAAATCTCCTTTGGCAAACATTGTTCAGGGTGCATCACTTTGTTGGATATCCTGAGCATATTCTCCTCGTCGATTGAACAGCAGCAAAGAGACTTCCAAATGATATCCGTTAGGAAATTCTCATTTAGTTTGATGGAAGTTTCATAAGGTTTCTTATCATCTGATTTGCTGGGCAAATCTCCTTTGGCAGTACTTATTAATAGCTCTTTTGGGCAAATCTCCTTTGGCATTAGCTGCTTCAGATATCTCTCTGGGCAAATCTCCTTTGGCATTAGTTTCTAGCTGTTTCATATTGCTCTTCGGGCAAATCTCCTTTGGCACTAAATACCATTTATATTTACTATTTTTGGTAAATCT



gi|33110997|gb|CF074338.1|CF074338 FE1_23_B01.g1_A002 Iron-deficient seedlings Sorghum bicolor cDNA clone FE1_23_B01_A002 5', mR

TGCCAAAGGAGAATTGCCCTG
|||||||||||| |||||| |
ACGGTTTCCTCTAAACGGGTC

EST sequence (targeted fragment marked with yellow):
CTTTTTTTTTTTTTGTTTTTAGATGGAATAGCAATTGAATATTAGGATTTTCATAAAAGCGCAAGTGTACGAACCCAAACAGGCACACATGCATTGGACAAAGCACACATAAGCAATACATTCTACCAACAATGATAACCGTGTTACTAAAATATTACAAATGTGGATTGCTGGGCAAATCTCCTTTGGCAGAGCAAACCAGATCCAGCAAAGTAAAATTCAATTACTCTGCCCAAGGAAAAAGGGCCCGGTAATCCTCGACTCACATATGCTTGCACACGGGAGACGGCAGTGCACTACGGCACTCTGCTTATTTGGCTTCCCTGATGACTGCCTTGTTGCATCCCAAGTTAGATCAATGCAAAATTTATATGCATAGAACTGTTGCTAGATTTGACAGCATGGCATGGAAGCTGGTATCAACATATCCGCGTGATCTTCTCTT



gi|9852079|gb|BE597006.1|BE597006 PI1_60_B12.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

TGCCAAAGGAGAATTGCCCTG
|||||||||||| |||||| |
ACGGTTTCCTCTAAACGGGTC

EST sequence (targeted fragment marked with yellow):
GCACGAGGTGGCGGCGGGTTGGAACTGCGACAGGGTCAGCTGATCTTGCCGGATAGACCACTCTATTGTGTTATCTCATTATAAGTGAAGCCCCCACTGATGAAAAGCTGTTATCTTGGTTGCTTTCTTGAGGAGTACTGGTCACCAGATGGTAGCATGTTCTCTTCTGTGTGATTGGGCTTGATTATGGACTGATGCTCCTGATCACTCTTGTCCTGTGATCATCCAGAGTATATCATCTGTCAGCTAGTCTTTCCTCTATTGGAATCACCTCCCTTTATGAAAGAATCCTTGTTATAGGGCAAATCTCCTTTGGCAAACATTGTTCAGGGTGCATCACTTTGTTGGATATCCTGAGCATATTCTCCTCGTCGATTGAACAGCAGCAAAGAGACTTCCAAATGATATCCGTTAGGAAATTCTCATTTAGTTTGATGGAAGTTTCATAAGGTTTCTTATCATCTGATTTGCTGGGCAAATCTCCTTTGGCAGTACTTATTAATAGCTCTTTTGGGCAAATCTCCTTTGGCATTAGCTGCTTCAGATATCTCTCTGGGCAAATCTCCTTTGGCATTAGTTTCTAGCTGTTTCATATTGCTCTTCGGGCAAATCTCCTTTGGCACTAAATACCATTTATATTTACTATTTTTGGTAAATCT



gi|9852079|gb|BE597006.1|BE597006 PI1_60_B12.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

TGCCAAAGGAGAATTGCCCTG
|||||||||||| |||||||.
ACGGTTTCCTCTAAACGGGAT

EST sequence (targeted fragment marked with yellow):
GCACGAGGTGGCGGCGGGTTGGAACTGCGACAGGGTCAGCTGATCTTGCCGGATAGACCACTCTATTGTGTTATCTCATTATAAGTGAAGCCCCCACTGATGAAAAGCTGTTATCTTGGTTGCTTTCTTGAGGAGTACTGGTCACCAGATGGTAGCATGTTCTCTTCTGTGTGATTGGGCTTGATTATGGACTGATGCTCCTGATCACTCTTGTCCTGTGATCATCCAGAGTATATCATCTGTCAGCTAGTCTTTCCTCTATTGGAATCACCTCCCTTTATGAAAGAATCCTTGTTATAGGGCAAATCTCCTTTGGCAAACATTGTTCAGGGTGCATCACTTTGTTGGATATCCTGAGCATATTCTCCTCGTCGATTGAACAGCAGCAAAGAGACTTCCAAATGATATCCGTTAGGAAATTCTCATTTAGTTTGATGGAAGTTTCATAAGGTTTCTTATCATCTGATTTGCTGGGCAAATCTCCTTTGGCAGTACTTATTAATAGCTCTTTTGGGCAAATCTCCTTTGGCATTAGCTGCTTCAGATATCTCTCTGGGCAAATCTCCTTTGGCATTAGTTTCTAGCTGTTTCATATTGCTCTTCGGGCAAATCTCCTTTGGCACTAAATACCATTTATATTTACTATTTTTGGTAAATCT