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mature miRNA: TGCCTGGCTCCCTGTATGCCA
species: Festuca pratensis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|237618618|gb|GO889024.1|GO889024 RDD_cLiFpEfI12nb_16x1_2_K16_cLibkit5_F08 RDD_cLiFpEfI12nb_plant Festuca pratensis cDNA clone

TGCCTGGCTCCCTGTATGCCA
||||||||||||||.||||||
ACGGACCGAGGGACGTACGGT

EST sequence (targeted fragment marked with yellow):
ATGCTCGAGATAGACTGGGATGAATCATCGTCGGTGCCATTAAGAAACAGACATGTTAATGCTTGGCAAGTCGAACTCGTTGGGTACCCTCCCATCCTGAAGAAGTTCAAGTACAATGGCACGACTGCTGGACTTGAATACCAGAACCTGGCAATGCTACTGGATTCACCACCTGTTCCTGCTGGCATGCAGGGAGCCAGGCACATTGGCCCCACTGATCAGTTTCCCTCTTCATCTACTACAGAGTTCACAACTCAGCTGCTGTTCCCACAGGATCGTCAGATTCCGCCGAGTTCCAGTGGTGGCTCCTCTGAGGTTGTGAATCCTGAAGAAATAGGTGGATCTCCACCCAACAATTCAGTGAACATGCCTCCTTCTGAATTTCCTGAAGAAGTGAGGAGCATACAGCTGTTTGGCACCACGATCACATCACCTGTGCAGAATGCCACCAATGGTTCCTCGGAAGAAGTGAACCAAGTTCCTGATGCTGCTGTGGCAGATAGAACTGCTAATGAAGATGCTTCTTCCACAAGTCCATTTGACCTCAATCTTGGTACAGATGATGGCCCCAGCCAGAATGATTCTAGGGAAGAAGCCTGAATTGTGGCATATATACACTGCAGTGGAAGTGAAAGTAAGTTTGGTGAGGCTGCTATTCTGCGTTACACTACACAAGGTTCTCAGACTTGAATTCGGGAGGGGATACTTGTGGATGGCTGCAAGCCAACAAATTGAAGTTTGACGCTTCATGGGCATGTTGGCTGCTGCACGACTAGGCTGCCATGTTTTTTCTTTCACTTTTTGTAATAGTTTGGCTCTCACGCATCTGAACAAGAAAACATATGCACCATGTTATATC