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mature miRNA: TGCCTGGCTCCCTGTATGCCA
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|211863721|gb|CO484471.2|CO484471 GQ0206.B3_K22 GQ020: Clean ROOTS systems -Diurnal cycle Picea glauca cDNA clone GQ0206_K22 5

TGCCTGGCTCCCTGTATGCCA
||| ||||||| |||| ||||
ACGAACCGAGGAACATTCGGT

EST sequence (targeted fragment marked with yellow):
CTTTACTCCGCGCTCTTCCTTTCTCACCGCGCTCTTCGTATATATATTCTCACATTCCCCCATCCATCTCCATTCCTCAGACGAGGCCTCTGTGGCTTCTTATCTTCTGTTCATCATCAATTTATAAGCTGTGGGCTTCTTCAGATCTTCAAGCTTTCTCTGTCTATCCCTAATGGGTTATATTTCATGATGTTGCAGAGGGTGGGATGAGGAGGAGGAGGAGGATCAGGAGGGTTAAGGCTGGGCATTATATGCAAAGGTTGATGTGTGCCTGGCTCCCTGTATGCCACATACAAGGCCCAGTCATATGTTGATTGGCTTCATAGGTGGCTTACAAGGAGCCAAGCATACGTTTTCTTACCCTCTAAGCTTGATGTTTTTTACTTTTCTTTTCCATGCTTCTGTCATTTATTTTACTTCCTTTCATTCATATCCAGAGGTTTCAAGTTCCACACTCCTTTCTGGTTGCTATGTTGGAACAAGTTTTGTAAATATATGATTCAACTAGGATAGTGGATACTTTGCTTAAAACTAGAGTTAGGGTTAGGTTTTTTTTTAAATGAAGGATCCATATTCATATGTTTTCAGTTGTAAAGAAGATAAACCTTCTGGACATGATAAATATTAGTATATTACACTTCAGATGCACAATATAATTTACTTAAACATTAATCTTGGTATATGTTATTCTGTATTATGAATGCAATGCAGGCATTTCTTTGGTGTCATGATAAAGTCAATTTGGAGAAGTTCAAACATCTCCTACCTTATAAGAGAAAAGGAATTGTGAAAGCTAGTTTTAGAAGGTATGGATTCAGGTCTTCACAATG