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mature miRNA: TGGAAACCTGACGGGCCTCCA
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|225909058|gb|GO363402.1|GO363402 GQ03315.SP6.r3_C18 GQ033 - Terminal leader (Normalized library) Picea glauca cDNA clone GQ03

TGGAAACCTGACGGGCCTCCA
|||||||||||..| |.|||
TCCTTTGGACTGTTCTGGGGT

EST sequence (targeted fragment marked with yellow):
TTCAACCTCAATCAGTATATTCTTTACTGCAATCCTTTTGTTTTACATAACAGGCAGAGATGAGTTTCTGTACACACCACTTTCGGGATTTCATGGGGTCTTGTCAGGTTTCCTTGTGGGTGTGAAACAAATTATGCCTGATCAAGAAATTACTGCATTCCATGTTTTCAAGATGCAAGCAAAGTGGCTACCTTCCCTTATGGTTTTGGTATCCATTGTTATAAGCTTTTTAACAACAGAATCAATGCTCTACCTCCCCTTTGTAATATTTGGAACTTATTGGAGCTGGCTATACCTGCGGTATTTTCAACGGAATCCAGAGACAAATTTGAAGGGTCATCGAAGTGATGATTTTGCTTTCTCTACACTTTTCCCTGGCTTTTTGAGACCAATAATAGATGCATTTGCCTCAATATGCCAAAAATTATTTTGTGGAAATGCTCAGAGTAGCAGTGACGAGCAAGACAGCTATGTGTTAAGTGGCATATCTTTGCCGGTATCTGACCCTATAGAGGCATCTAGGAGGAGACAACGAGGGGCACGTGCCTTGGAGGAAAGGTTAGGTACTACAAAGGATGAAGAAGAAGCAGTTGGTGGAGATCGATCTCATGAAGATGCATCTGAAATTGTATAATGATTTATCAAGGGAGTTTTTTTATTCACCCAATATTAACATGGTATATACAAGGACCAGATGTTGAGCCATATTAATAGAGAGAAAATTGCCTTGAAGAACCCATTTCAAGCATGTTCTGATAACAATTTTTTTAGAGCGTACTTGGATGTTTGTTTCAGTTCTTTGGATGCTTTTTACCATATTGAAACTAATCAGGATAGAACTATTGACCATTCAAAAAA