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mature miRNA: TGGCTGAAACTTTGGGCTCA
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73880282|gb|DT483020.1|DT483020 WS02521.B21_G22 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02521_G22 3', mRNA sequence 

TGGCTGAAACTTTGGGCTCA
 ||||||| ||||||||||
TCCGACTTAGAAACCCGAG

EST sequence (targeted fragment marked with yellow):
GAGCCCAAAGATTCAGCCTGATACACTGAGAAATTTGATGGACATGAAGGCAAACCTAGGGTCATTTGCAGCTGCCCTGAAGAGCAAAGACGTATGGGTTATGAATGTTGTGCCTGAAGATGGACCCAACACTCTCAAGATAATATATGATAGAGGCCTGATGGGCTCTGTGCACAGCTGGTGTGAATCCTACTCGATATACCCACGAACTTATGATTTACTGCATGCCTGGACTGTCTTCTCCGACATCGCTAAGAAAGATTGCAGTGCTGTGGATCTGTTAATTGAGATGGACCGCATCTTGAGACCCACAGGCTTCATTATTATCCGTGACAGTCCATCAGTAGTGGAATTCGTAAAGAAACATATGTCAGCACTGCATTGGGAGGCAGTGGCTACAGGTGATGCTGAGGAAAACGAGCAAGGTGAGGATGAGGTGGTGTTCATCGTCCAAAAGAAGATGTGGCTAACAAGCAAAAGTTTCAGTGTCACTGAATAAAACAGCCAACCCGGATTTCCATACTCTCGCCCTTTTACCTGAACATTGCAGGGCTTAAGGTAGCCGTGGTTTATATGATTTTGTAGCATTTGATGTTGAGTTTATTTCTTCTTGAGCAATCTTATATGAAAGCAGAAGTTTTATCCAGGTTATTTCTTTTGTGTTATTTCATTATAAATCCAAATTGTTAGTTTGATTAGCTGACAATTGATTAAATTAAATCAAATCAAGGCTGCTATAGGAATTATGGAAACTGATCCTCTCATTATTTTATAGGAGTCTCCCGAGAGATGATTGTATTTTGCATTAGTTTGAAATGTCATTTTGAGTGTTGATATTTTCTAAAAAAAAAAAAAAAAAA


gi|73881344|gb|DT484082.1|DT484082 WS02524.B21_G07 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02524_G07 3', mRNA sequence 

TGGCTGAAACTTTGGG-CTCA
||||||||||| |.|| ||||
ACCGACTTTGACATCCTGAGT

EST sequence (targeted fragment marked with yellow):
CATCCAGGAGCCACAAGCAACAACATTTTGGGTTTGATAATATGGATACTCCAACACCGAGATCCTCAAAATCAACTGCTTTTGTGCCAACGAGACAAGCGCGAACTCCTCTACATAGAACACCACAAGCCAACAGCCCTAGCTTGTCAAAATATTCAATGGCAAGAGCCAGTGCAGCAAATTCTCCTTTTAACTTGCCATTGAAGGATGACGATAGCCTCATGAGCTGCCCACCATTTTCAGTGCCAAACTACATGAGTCCTACAGTTTCAGCCAAAGCCAAAGAAAGAGCTAATAGCAATCCCAAGGAGAGGTTTCCAGGGACCCCAACCAGTGAAAAGAGGAGACTGTCATTCCCTCTTACACAGGGCATTGGGTCTTTCAAGTGGAACAAAGGCTCCTTCACAAGCAAGGATTCAAGTTCTCAAAGGGGTTTAGATAGGCATCAGTCACTTCAATCCATAGGAAACTTGAGTGTGGATTCAACCGTCTCGATGCCTGCCACGCGAAAGCCATTTAACCGATTCGTGTGATCTTTTTTCCTATTTTCTTAACATCTTTTTTCTTCATATTTTTCTTGTTATTTGATTGTGTGTATGGTGATATTGAGTTACAAAATAGTGGTGTAAAAATAATGAAGAGACAAATAGGAAATGATGGTGTGAATGTAACAGGATTTGCTTAAAGAACAACATGTGACAGGTTTTGGATTGCTTTGAAACATTTTATCTTCTCTCTATGAGAAGTGTGATGTTCCACAAAAAAAAAAAAAAAAAA