miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: TGTCAGAAGAGAGTGAGCAC
species: Gossypium hirsutum
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|164272834|gb|ES839900.1|ES839900 UFL_059_30 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
GGCACGAGGGAAACTTCCGTTGTAACTTCGATTCAACCGGTTTTGATATCGAAGAAAGCTTCGATAACAACAAGTTCATGTCCTCAAACACCTCAATGTCAAGTTTATGGTTGTAATAAGGATCTTAGTTCATCAAAAGATTATTACAAGAGGAACAAAGTTTGTGAGATTCATTCTAAGACAGCCAAAGTTATTGTTAATGGAAATGAAATGAGATTTTGTCAACAATGTAGCAGGTTCCATTTATTGGCTGAATTCGATGATGGTAAGCGTAGTTGTCGTAAACGACTAGTAGGACATAATGAGCGTAGAAGGAAGCTTCATTTCAGTACCCTTCCCGGAAAATCACATAAGTTGTTGCAGTCATATCGAGAGAGATATGAACATGCTGATCAGGTTAAATTCAGAGAGAAACCGGTTTATGATCCTCAGTCGGCAATATCGATCATAAATGGCAAATTGGTGTCAAAGAAACAACACATCCCCGGAAGTTTTTCGTCGGCAACTGAAGCCTTCCATGCTCCTAATGAGTTATCTGGTCTTTCTTGCTCGGATTGTGCTCTCTCTCTTCTGTCAGCTCAATCACGAGACTTATCGAGCCATGCGACCGGATTTCAATTGACCGAGCCTTTTATCAATCAACCTAGCCACCACCATGGGAATTTGTTTGGAAAATGCCAATGGTTTTTACTCATGTGGGATGAACCCCATGGGAAGTAACCCAAGCAAGATCCCTTTCGAAACCCGGGTTCTTTCAAGTCCCAAATGGAACCACTGGTGAATTTACTTCAACTAATCAACACTTCTCCAAAAAATGGAAACAACAAAGGAAGTTCAATGCACCTAAAGGCCTGAAAACAAAAACCTTTTTAATTTCCTTAAAACTTAATCCAAAAAAAAAAGGGAAAAGGTTTGTTCCGTTGTTTTTTCCAGGGTTCAGAAAATGTTAACCCGAAATCGGGTTTTCCGAAAAATTTTCTAAATATTTTGAATTTTCCTTGGAAGAATCTATATTTTTCCCAATGGTCGGGAAAATGGGTCAAAAAACGGGGGTTTGGAAACCCAAAAACTTTCTCGGGTTTTTAAAAGATCCCCTAACCACTTTTTGGGGGGCATTAAAGGGGGCAAATTTGAAAAAAAGAACAAAAATAAAAATTCCTAAAATTTAAAAAATAAAACCCCTTGTGAAAAAAAAGTTTAAAAAAAAGGGAATACTTGGAACAACCTTAAAATTGTTTTTTTTTTTTTTTTTATTATTAAAAAAAAAAAAAAAAAAAAAAAACCCAAAAAAAGAATTTTTTGAGGAGGGGGCCGCCCGGGCCCCCCCGGAAATTTTTTTCCCCCCCGGGGGGGGGGGGAGCCCAGAACAGAAGAAGGATGACCCGCCACCACTTCGCCCC



gi|164345361|gb|ES831037.1|ES831037 UFL_340_18 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| |||||
ACTGTCTTCTCTCTCTCGTA

EST sequence (targeted fragment marked with yellow):
GGCACGAGGTTGAACTAATGCTGACAGGGACATAAATGGAACTTTATGCAAAACCCAGAAATGGCAACAAGCAAAGCTGAAGGGAAAAGGAGACTGAAGGAAATGGGGGAGGAGGAAGAAGAAGAAGAAGAGGACGAGGATAATAGTACTACTGGTGACGATGACAAGAAGAAAAAAGGCAAAAGAGGGTCCAGTACGGTGGTCGGAGGCTCCTGTCTTCCAGCCTGTCAGGTCGAGAACTGCACTGCCGACATGACCGATGCCAAACGGTACCATCGGCGACATAAGGTGTGTGAGTTCCATGCCAAGGCTGCTGTGGTTCGAGTTGCTGGGATCCATCAACGCTTTTGTCAACAATGTAGCAGGTTCCATGAGTTATCAGAGTTTGATGAAACAAAAAGGAGCTGTCGACGACGTTTGGCCGGACACAACGAGCGCCGTCGGAAAAGTTCATCGGAATATCATGGAGAAGGCTCAAATTTTTAAAACTAGCTAAGAAGGCAAGCCTAAGATATATATATATATGCAAAAAAGAGTCAATTCAGTCTTGAATTTAATATGATCCTATGTATGGGAATGGACTTTCCCAGATGATCATGTAACTATATGGAATTTACTAATTAAGCATGCTCTCTCTCTTCTGTCAATCTTAATTTTTCCCCCTATAATATTGTCACCTTTTTCCTGGCTTGCTCTCCNNAAANAAAAAAAAAAAAAAAAAAAAAACAATAAATAACTCCAAAAGTATTTCTTAAGCGGCCCCGCGGCCCCTCCATTTTCCCCCCGGGGGGGGGTTACCCGGTAAAGGGGCCCCCATTTCCCCCCTTAATGGAGTCCTTAATACAATTCCCCGGGCCGCTCTTTTTTAAACCTGCCGGGACTGGGAAAAAACCCCTGGGTTTCCCCCATTTAAATTTCCTTGGCCACCCTTCCCCCCTTTCCCCCCTCTGGGGGTTAAAAACCAAAAAAGGCCCGCCCCCCGTTTCCCCCCTTCCCAACAAGTTTGGCCCACCCCCGAGATGGGGCAAAGGGGGAAATCCCCTTTTTTTTAAGGGGTGAAAAAGGGGGTTTAAAACCCCCCCTGGATTTCCTCAATTTTGTTGTGTGGATTTTTTTTAAAAATTTCCCCAATCTCCACCGGGGCTCTTTTTTTCCCGGCCCCCCCCCCCGTCCCCCCCCCCCGGGCTCTTGTTTCTTTGTGAACATATAAAAATCCCCCCCCCCAATCCCCAACACTGTTTTTGTTGTAAAGAGGTTTTTTTTTCCTTTGTTCTTTTTTTTTAAAAAAAAAAACCCCCCCCCCCCCCCCCCCCCCCCTCCCTCCCCC



gi|109862269|gb|DW491246.1|DW491246 GH_RMIRS_084_H12_F Cotton Normalized Library random primed Gossypium hirsutum cDNA, mRNA seq

TGTCAGAAGAGAGTGAGCAC
|.||||||.||| |||. ||
ATAGTCTTTTCTGACTTTTG

EST sequence (targeted fragment marked with yellow):
GAGAAAGAAGCGGCCTTGAATTTAACTGTAGATGCCTTTTGTAGATTAGTCAAGCAGCATGCTAGTGTAGCTCAATTAGTCACCCAGTATGAATGCTGATCCTTGATTCTGTTTTCTTAATATTACATAGTTTATTTTGTCTGCAATATTTTTTCTGGGGTTTTCAGTCTTTTCTGATATAAGCAAGAACTTTTTGTATCCTTTCACATTGTTAAGTATTGCTGCCATCAGTGGCTCTGTTCTGTCCAATCTCCTCCTACTTGTTTGAGTGTTTAGAAACTCTGCTTCAGTAATACTATAACTTTTTTGTCATGTTTCAAAGTAGAGTTAGTTGTATTCTGAAATATGTGTATCAAGATACTTTCTCCCAATATTTATGTTCAATGCATCTCTTGATATTTAGTCATGCTTTTTGTTTCATTCTTGTAATCTTGTTGTGACTTAATCTGGTATTAGAATGGTTTTCATCTTCCATTTTCTTCAATTTTTTAAATTTATGATGCGGTTGGCTTTATTATTAGCCAAGATCCATACCACGTAACTGAATTCTGATTGGACTACTTAAACTATGCTGACACTGGCTGTTTAGTTGATATAATTTTCTTCTTCCAAACATTAACTTCCTGTGAGGATTGTTGTAATTATAGTTAGTTTTTTGAAGTATTTGAAAATTTCATCAGCCTTTGCCTTAAATTCATAAAAGAATAGTCAATTAATCAATTTCTTCGTGCCATTTGAATTATTTATCATTTTTGTATGAATTATTTAGATCATTCCTGCCCTTGTCTCGTCCTCTGCCTG



gi|164273618|gb|ES842145.1|ES842145 UFL_084_81 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
ATCAAACACCTGCGAAAGCTTCCATTTGCCAGCCAGTCCTCGGGCGTGTTGCACCTTTCTCGGAAGGCAATGGGGGCTGCCAGAGCATGTTATGTGACAGGCTAACAACTCAAGTTCGGGATTCGGATTGTGCTCTCTCTCTTCTGTCATCTCCACTGTCGCATACATCTGGGATTGGGTTGAGTAACATGGTGCAGCCTCAGTCATTCCCCTTGGTGCAATCCTTGGGTCCGAGCCTACAAGACCACATTATAGAGCCTATGGGTTCGGTCGTTGCTAATGGCTGGGAGACTACGGTCCATGGCTCAGGAATGTTTCACATGGGTTCTGGTGGATCATCTGCAAGTGAACCCCCTGAAAATCTTGGCTTTCATTGGCAGTAGGAAATTTGAAAGTCGCCGTAACAATAAATTGCCAAAGCTATAATATTGGTACATTCGAATTTAAACTCACGAGTTTCGGGGTTTTTTTCAGGTGTGTTTTAATTTGGAGATGTTAATCTATTGAATGTTACTCTTAAAAGTAGATGCGAGTATTACTTTGATTAAACTTTGGGTCCCAATAATAGACATTTAGTTGGAATGATACATGCAATCATTTTAGTAGTGGAGAGAATCATGTTTGGATTTAAAAAAAAAAAAAAAAAA



gi|109849402|gb|DW478381.1|DW478381 GH_RMIRS_013_H04_R Cotton Normalized Library random primed Gossypium hirsutum cDNA, mRNA seq

TGTCAGAAGAGAGTGAGCAC
||||.||||||||||| |
CCAGTTTTCTCTCACTCCCG

EST sequence (targeted fragment marked with yellow):
ATCAGGCAGAGGACGAGACAATCTCCACTGCAATCACCATCATCAGCTACTGTCATTTCGGAGCCCTCACTCTCTTTTGACCTGAAATCAAGAACATCAGTTTCAGAAACAGGGGCTTCAACACTATCATTAACTAAACCATCTCCACTGCAATCACCATCATCAGCTACAGCTATTTCGGAGCCCTCATTCTCTTTTGACCTAAAATCAAGGGCACTATCATTAGCCGAGCCATTACCATCAGGGCCAGGGACAGGAGAAATCTCATCACTCTGTCTAGCACTGGAATCAACAACAGCAGCTTCTGAAACAGTGTGTTTAACTATATTCTTATCAACAAATACATCTTCAGTCAATTCAGAGATTCCATTCTGATCAGCCATCCCAGCATTAGTAGCAGAAACAGGACCGCCCTCGACAAGATCCCCCGATTTTTGTTCATCAACGGGAGCCTTCACGTGGAACAGTTCCACTGATTCTTTGTCCTGATCCACAATTCCAATACAAGATTCATCCTTTTCTACCAAATCAAATTTAGATTTGACTTCTCCTTTAGTCTCCAAACTCTCAAACCCCTGATCTTCCACACCAATGCCATTGCCGTTTTTGTCAAGATTCGACTCCACTAGATCTCCGTTTTCGGCTTCGTTTCCGTTAACGTAAACGTAATCACCGTCACCATCGGTGTCTTCCCTGACAGTCTTCCCATTGGTGCCATTGGAATCTTCCTTTTCGTTCTCTTTCCCGTGATCTTCCTTCACCACAGTCTCAACCTTGGACGAAGACGATGCCGCCATCTCCTCCTCCACCGCATGCACGGCCATCACTTCATCCACAGTCATATTCGCTCCAAATTA



gi|78337780|gb|DT558054.1|DT558054 EST1068694 GH_TMO Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
TTTAGTACGGGAACCAATGCTACGGATTCATCTTATTCTGCCACTCTGAGCACATCATCTCCTTTATCTGCAAAGAGATCCAAACTGAATTGTCGAAGCACACATGTTGTGCGTTGCCAAGTCGAAGGTTGTAACCTTGACCTTTCTTCTGCTCGAGATTATCATCGGAAACACAAAGTTTGTGAAAGCCATTCAAAGAGTCCGAAGGTCATTGTAAGCGGTCTGGAACGCCGGTTCTGCCAGCAATGTAGCAGGTTCCATGCGCTGTCGGAATTTGATGTAAACAAACGAAGCTGTCGTCGGCGACTTTCCGACCACAATGCAAGACGCCGCAAACCACAAACTGAAGCTACCCACTTCAATGCTGCAAGGATTTCATCATCATATGATGGGAAGCAACATACGAGTTTTGTCTGGAATGAAGTCCCAATTCTTCATAATGTTAGACCCAAGAAGAAATTTGCGATGGAAGGCACGTTCTACTCCAAGTCCTCTCGAATGACAAGTTATATGCCTATGAAACTTGGAAACATCAATGAACAGGTGCAACTTCCAATCAATCAGCTGTCAAATCCCGTCATGATGCGATGTAATGATTTCAACAAATTCCTACCCTCTAAGGGTAAGCAAAACACGGCTGAGGTTCTCGATGGAGGTGTAGAACAATCTATGGTTGCTTCAAGCACCGGCACAGCTCAAGAACTTCACCGTGCTCTCTCTCTTCTGTCAAATGATCCCCAGGTTTCAAGTGAACCAAAACACGGTTCACTTACCTACCCCATTATGCATGTCG



gi|73861547|gb|DT464286.1|DT464286 GH_CHX15G02.f GH_CHX Gossypium hirsutum cDNA clone GH_CHX15G02 5', mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
GCGGCCGCGTCGACTGATAAACCAGTGATGGAACTGATGTTACGAGCTTACAGGTTTTTTGTCAAATTTTGCAGGTGTAGAACAATCTATGGTTGCTTCAAACACCGGCAGAGCTCAAGAACTTCAACGTGCTCTCTCTCTTCTGTCAAATGATTCCCAGATTTCAAGTGAACCGAAACACGGTTCACTTACCTACCCCATTATGCATGTCAACCTTACCAGTACATCTCAGCCAGCAATGAATGCAATCCCACAAGGTTTTCAACATGCCTTGCCAGAAAACTGGCAGATCGAACAACAAGAAGAAACAACCACCAAGTCCCGCATGCATACTAGCGACGTCGACGACCGTTTTCGGGAGTTCCTATTGCTTAAGGCTCCATATGATGTTGGCTTTTACTCATGAATCGGACAATGTCTCTTAACACCATGTATCGATCTATGGGTATATGCAAAAATTCAGTTAACTGAATTAATTTAACCAAAATAGATCAAACCCGAATCTGCAAAAATTCAGTTAACTGATTAAACTTGAAGTGAACTGAATTAATTTAACCAAAATAGATCAAACCTGAATCTGACTCGGCCTCGAACATCAATTTTACCAAAAAGTACATACCAACTATAGCCCCTATGCTGCTCAATGTTCTTTGCAAGTCATCACTAATGCT



gi|164336040|gb|ES806239.1|ES806239 UFL_639_51 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| |||||
ACTGTCTTCTCTCTCTCGTA

EST sequence (targeted fragment marked with yellow):
GGCACGAGCTCCTCTTTTCTTTTGTGATTTTTTAAGCAAAACCCAGAAATGGCAACAAGCAAAGCTGAAGGGAAAAGGAGACTGAAGGAAATGGGGGAGGAGGAAGAAGAAGAAGAAGAGGACGAGGATAATAGTACTACTGGTGACGATGACAAGAAGAAAAAAGGCAAAAGAGGGTCCAGTACGGTGGTCGGAGGCTCCTGTCTTCCAGCCTGTCAGGTCGAGAACTGCACTGCCGACATGACCGATGCCAAACGGTACCATCGGCGACATAAGGTGTGTGAGTTCCATGCCAAGGCTGCTGTGGTTCGAGTTGCTGGGATCCATCAACGCTTTTGTCAACAATGTAGCAGGTTCCATGAGTTATCAGAGTTTGATGAAACAAAAAGGAGCTGTCGACGACGTTTGGCCGGACACAACGAGCGCCGTCGGAAAAGTTCATCGGAATATCATGGAGAAGGCTCAAATTTTTAAAACTAGCTAAGAAGGCAAGCCTAAGATATATATATATATGCAAAAAAGAGTCAATTCAGTCTTGAATTTAATATGATCCTATGTATGGGAATGGACTTTCCCAGATGATCATGTAACTATATGGAATTTACTAATTAAGCATGCTCTCTCTCTTCTGTCAAAAAAAAAAAAAAAAAA



gi|164251838|gb|ES847167.1|ES847167 UFL_141_21 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
ATCAAACACCTGCGAAAGCTTCCATTTGCCAGCCAGTCCTCGGGCGTGTTGCACCTTTCTCGGAAGGCAATGGGGGCTGCCAGAGCATGTTATGTGACAGGCTAACAACTCAAGTTCGGGATTCGGATTGTGCTCTCTCTCTTCTGTCATCTCCACTGTCGCATACATCTGGGATGGGGGTTGAGTAACATGGTGCAGCCTCAGTCATTCCCCTTGGTGCAATCCTTGGGTCCGAGCCTACAAGACCACATTATAGAGCCTATGGGTTCGGTCGTTGCTAATGGCTGGGAGACTACGGTCCATGGCTCAGGAATGTTTCACATGGGTTCTGGTGGATCATCTGCAAGTGAACCCCCTGAAAATCTTGGCTTTCATTGGCAGTAGGAAATTTGAAAGTCGCCGTAACAATAAATTGCCAAAGCTATAATATTGGTACATTCGAATTTAAACTCACGAGTTTCGGGGTTTTTTTCAGGTGTGTTTTAATTTGGAGATGTTAATCTATTGAATGTTACTCTTAAAAGTAGATGCGAGTATTACTTTGATTAAACTTTGGGTCCCAATAATAGACATTTAGTTGGAATGATACATGGCAATCATTTTAGTAGTGGAGAGAATCATGTTTGGGATTTAAAAAAAAAAAAAAAAAAACTCGAAAGTACTTTCTAAAAGCGGCCCGGCGGCCCCCATCGAATTTTTCCCCCCCGGGTGGGGGGTACCAAGGAAAAGGTGTAACCCAATTTCGCCCCTAATAGGGGAAGCCCGTAATTAAAATTTTCCCTGGGCCCGCTCCTTTTTTTAACAACCGCCCGGGAACTGGGGAAAAA



gi|109875775|gb|DW504749.1|DW504749 GH_TMIRS_087_C02 Cotton Normalized Library dT primed Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
ACCACAACTTCTCAAGGAGAATGCTTCACGGGAGTTAGTGACTCGAGCTGTGCTCTCTCTCTTCTGTCAAATCAACCATGGGGCTCCAAAAACAGAGCTTCGGCTCTTGGTGTTAACGACATGATGAACACCGAACCAATGCCCCATGGCACATTCGTAAACCCGTTTTTGCAAACCTCGGAGCCTGCTGATGCTCAACATTTCGGCAGGCTTGAGTTGCCTCAGCAAAACAGCGTGCAACACATGGAACTTGAGCAGCACTCCAGATCATTTGACACATCGGCACAGTATGTTCACTGGTCACTCTAAGTTCGTCCCATTTCTCTTTCCCATCTTGTTGTCCTGTTTTCGTGCCAGTTCTTATGTTGTATTCGTACTTTTCCGAGCATCAGATTTTATGTTTTAACGGAATAAACCCGAACTATCCCCTGTATTTGCCATTGTTGTTGCCTGAATCATTAAGTCAGGCATTACTAGATGTTTTGGTATTTATCTATGATCAATGGCGGG



gi|109872376|gb|DW501351.1|DW501351 GH_TMIRS_055_G11_F Cotton Normalized Library dT primed Gossypium hirsutum cDNA, mRNA sequenc

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
GGGCAAACGGATAAAAGTTGAAGATGGGACGGATTTTAGCCCTCTTTCTGCCATACCTGTCAAGAACGGGGGGCATTTTGAGAAACATTTTCCCTATTTCCATAATGGGCCTAGTGCAGTCACTGGAGGCATTTTAGGTGAGAACAATGCACGATATCCTCATGGTTTTGATGGGACGTATTCTGGATCACGGCCTTTGTTCCAAGGCACCTCCTCGTCAGCAAGTGAAGACATCAATGTTTTCGATGCAGCATTGAGCATTCAGGGATTATCTGGGATTTCAGATTCTGTTTGTGCTCTCTCTCTTCTGTCATCTCAATCACACAGCTCTTCAAGCCGCTCATCAGGAATTCCCATGGCCCGTTCCCTGGTTATTCCGGGCTACCATAACCATCACATGACTCAAGTCTCGGAAAAGCTCGACAGAGTATCGAATACGATCTCTTCATCAGGGATCAATTCAACCGAAGGCGGTAACATGGGTGCTATACTGATATCTGATAGTAGTGAAGCTGCCAACTTCAATATCACTGATGGACTATCATGCAAAGATGGACCGACGATCAACTTGCTTCAGCTTTCATCGCAGCTTCAGCGAGTGGAACATCAAAGACAATCTATGCAAGAAGACAATGAAAATGAGACCTACCTCCGAATCGTTTAAGTACAGATTAAGACAAGTTCAATGTTGCTGAAAGCTGCACAGTTCTTGAGATGTCCGAATAGTCGGCGTAACATAGATGGAAGCATGATGAGAAAGTTCAGCAGCTGCTAACTGGATTCCCTACCCCACCCCTCCACTCGGTTAAGTTTCACCTAGACTTTTACAAGTTATTTTGTAATCGAAAAAAAATTGTTTGATTATGTTTCTCGAATTAAAAATGTTTTGAT



gi|109852935|gb|DW481914.1|DW481914 GH_RMIRS_033_E06_040_F Cotton Normalized Library random primed Gossypium hirsutum cDNA, mRNA

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| |||||
ACTGTCTTCTCTCTCTCGTA

EST sequence (targeted fragment marked with yellow):
CCTCTTCTTTTTCTCCCTTTTTTTAACCTTATTATCTCTCAAAGAGACTATTTTTTTTATTCTTCTTCTTCATAGAAAACCTCTTCATCTCATTCATCTCCTCTTTTCTTTTGTGATTTTTTAAGCAAAACCCAGAAATGGCAACAAGCAAAGCTGAAGGGAAAAGGAGACCGAAGGAAATGGGGGAGGAGGAAGAAGAAGAAGAAGAGGACGAGGATAATAGTACTACTGGTGACGATGACAAGAAGAAAAAAGGCAAAAGAGGGTCCAGTACGGTGGTCGGAGGCTCCTGTCTTCCAGCCTGTCAGGTCGAGAACTGCACTGCCGACATGACCGATGCCAAACGGTACCATCGGCGACATAAGGTGTGTGAGTTCCATGCCAAGGCTGCTGTGGTTCGAGTTGCTGGGATCCATCAACGCTTTTGTCAACAATGTAGCAGGTTCCATGAGTTATCAGAGTTTGATGAAACAAAAAGGAGCTGTCGACGACGTTTGGCCGGACACAACGAGCGCCGTCGGAAAAGTTCATCGGAATATCATGGAGAAGGCTCAAATTTTTAAAACTAGCTAAGAAGGCAAGCCTAAGATATATATATATATATGCAAAAAAGAGTCAATTCAGTCTTGAATTTAATATGATCCTATGTATGGGAACGGACTTTCCCAGATGATCATGTAACTATATGGAATTTACTAATTAAGCATGCTCTCTCTCTTCTGTCAATCTTAATTTTACCCCTATTTGTCTCGTCCTCTGCCTGATG



gi|24977058|gb|AJ513575.1|AJ513575 AJ513575 Gossypium hirsutum ovule first day the flower opens Gossypium hirsutum cDNA clone su

TGTCAGAAGAGAGTGAGCAC
||| ||||||||||||. |
ACAATCTTCTCTCACTTCTA

EST sequence (targeted fragment marked with yellow):
GAGTTAGAGCCGACTGGTACGAGCGGCGACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTCGAAAAGATTATAAGAATTATTTTAGAATATACAAAAATTCTCAGAAACAACAAATAAATATGTCTTCACATGGACGACAAAATCTTAAACTAGTTAAGTAATTAGTAGAAATGAAAAAGAATTTTTGTGTTCATACCAACAGCTCTCTTGTGAGGTATGGAACAACTCCTGACAGTATCTGAAGAGCACAATGACAAATGCCATAGATAACTTCATGATAGTCAATTTCACTTTTTATTAATTGATTATCTCCAGACATCACTGAAATAAGAATATGCTTTATGCCAACTTCAAGTTTTTCTGAACATTGCTCTATGACATTCATAGCAAGTCTCCTGGCAGCTTGAGTAACACCACTTTTATTACGACCTAATGCAGACAATATAACAAGTAAAAGATCATCTCGATCATCTTCACTCTCTTCTAACACCAAAATCATGATTGTTTGCATAGCTGATAAAACACTTTCTGGATTATCATCCCCAATGCCGGGGTAAGATCGCTTTCACTCACCCCGGACATAATGGACGAATTCCCGGGTACAACACAATGGCGCGCTACCGAGAAACAAAACAAGCCGCAGATTCGGACATGGGTACCAAGGACAACGACCCGAGGATAGGAGCGGCCAATGCACACATTCAAATAGGCGCGAAACAAAACGTTAAACTTGGAAACGCACCCACAGGAACGAAACACGATACGATGACCAGACAATTAGAAATAAAACACATCTGAGGCACAATCTTTTGTTCTGTGAGTTCATCCGCGTCGCCTTAGTGCACATGTCACACCCGAGACGACATCAGA



gi|164253448|gb|EX171997.1|EX171997 D04_F-25_031 Cotton 1-14 day post anthesis Lambda Zap Express Library Gossypium hirsutum cDN

TGTCAGAAGAGAGTGAGCAC
||.|||||||||| |||| |
ACGGTCTTCTCTCTCTCGGG

EST sequence (targeted fragment marked with yellow):
CGCGCGCCTGCAGGTCGATTCNNCTGGATCCAAAGAATTCGGCACGANTTTTGCAAAACCAAAAAGCAGCAGAAACTCCAGCATAGAGGCATTTGCAGTTTGTGAGAATTATTCACCGCCTGGAGGATTCAATCCAAAAGACTTGCATCGTCTCCTGGAAATGGTTGGCAGTCCCTCGGGTGGAGATGATCTAGATTGCAGTAGTGGATGGTTAGAAGGACCAAATAAGGTTTATATTCCATTTTTAGCTTGCGGGGACCTAAGCGGGTACGATTCTGACCGTTCATATCCACTGCCGAAAGTTGCTGATGGAACTTACCAGAGTTTGGATCCTGTCCAGCCTCCCATTGCCCCCCCTTATAAGAGAGCTCTTGAGATGAAGAAAGCTTCCAGTCATGGTATCCGAGACCTTGAAAAGCTTTCTTTGGATGCTTGAATTAAAAAAAGAAGGGTAAACCCATTGTGAGATGGGAGTTTTTAATGTAGAAACAATGATTACTGTTATTGTTTTATATACAATTTACATTCTTTCTATGAAAAGAAAGTGGAGTGCTCTTCTTTCATTAAAAAAAAAAAAAAAAAACTCGAGCAACTCCAGCAAACCTCCTCATGACCATTTCTTGCAGGGTCNCCATGGGGGGACTAGTTTCTCCAGCACTGGAATTTCTCCAGGAAAAGCTTCNCANGGGCTGGGGACTCAAGCTGGGCTCTCTCTCTTCTGGCAAANCAACCGGGGGCTCCNGNACCAGGGTTTNANTTTCTGGAANGGGGGGAAAAAACTGAAGGGCCTTGGGGGAAACCAAAACCTTTNNGGGNAGGGGGANCCTTTTCNAAGGCCCTTGGATTCAAGGGNGGAACCTGTCCAGTTTNCCAAAGGGGCCCCCTAATTNGGCAATCCCAACCTGTAAT



gi|109853117|gb|DW482096.1|DW482096 GH_RMIRS_034_E07_055_F Cotton Normalized Library random primed Gossypium hirsutum cDNA, mRNA

TGTCAGAAGAGAGTGAGCAC
||||| ||||||. ||||||
ACAGTATTCTCTTTCTCGTG

EST sequence (targeted fragment marked with yellow):
TGTCCTTTTTTTTTCAATCTCTATCATCAACTGGCCCCAAAAGATACTTTCTTGTACTTCATTGTATTCAAGAATTAGAACAAAGAGATGAAATGAACTTGTAGAAGGCAAATTTTATATATATATACTTTAATTTACTTAGTTTTTTTTTCTAACTATTTAAAATGCAGAGATTGGTTATCCATCATAGACCCCCAGTGATTTCAAGAAGGAAACTTCCTTTATTGGTTGTTGTGGATTCATTTCCATCTTTTGACAGTGTTTCAAGACTACCCATTTCTCCTTCATTACAATGTAGAAAAATATCAAGGTTGAGTTCAACGTGCAGCACGTTTAGAACACGAGCATCCAATGTAGGTATTGGATCCGGGGGCTATGAAGATAAGGATGAAAGAGAAGACCAAAGTATTGTGAAGGGCCCGCGCAGTGACAGTTCATCAGAAGCTTTGAAGCCTCCGAGTCAAATTCCTTACCCTCTTTCCGTTGCTGGTGTGCTATTGGGATGTACTTTAGTATTGTCACTAATTGCCTTTGCAAAAGGTGGACCTGCATCGCTCTTAGCAGCAATAGCAAAATCAGGCTTCACGGCTGCTTTCACATTGATCTTTGTTTCTGAAATCGGAGACAAGACGTTTTTCATCGCTGCACTCTTGGCCATGCAATACGAGAAAGGATTGGTTCTGTTGGGCTCGATGGGTGCTCTTTCTCTTATGACAATCTTGTCAGTAATAATCGGACGGATCCTTCAGTCAGTCCCTGCTNCATTCAAACCCCAGTTGTCTCGTCCTCTGCCTG



gi|5045402|gb|AI726550.1|AI726550 BNLGHi6118 Six-day Cotton fiber Gossypium hirsutum cDNA 5' similar to (AC004411) putative squa

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
GGATTGCAAGAGAAATGGAAATCAATTTCATAAAGTGCTCTTATCCTGCCTGCCCCTTGTGTTCAATTCTTTTTTTGTTGCATTTCATAATGATAAGTGATTAGTTTATGGTGAATGAAGGTTTCATTGTTTGGCGGAGTTTGATGAAGGAAAACGAAGTTGCAGGAAGCGTCTCGATGGACATAACCGAAGACGGAGAAAACCTCAACCTCAACCTCAACCTCATTCTCTATCTGTAAATCCAGGAAGATGTCTTTCCAATCTCCAAGGTTCTAGACATTTACCGTCCAGTAGTCCCCAAATATTTGCCACTACTGCCATAACCTCTTCTTGGTTGGGAACTGCCAAAATGGAACCTGATATAGGCTCGGAATCCANCCGAAATGGTTTCTTCCGTGGATCCTTTCCACACAGCTACAAAAGGGAAGAACAGTTGTCATTATTACAAACCAATCATTCATCACTCCATGGAGTTTCTGTTTATCAGTTACCTTTATTAAGCAATGGTGTAGGCAGCAGCCAAAAAATGTTCTCAAATGCGATCGACTCCGATCGTGCTCTCTCTCTTCTGTCATCACAGCCGGCTGAGAACTCGGAGATTGGTTTGAAAACCATGGTGCTATCTGGTCCAACTTCATCACTGATCCCAAACATGCAATATAATGGTGTANCAATGGAAGGTGAGCATGTTTTTGGCACTNNGACGCATGATAACACAACATCTATGGACAAAAAATGTTTGGATCTGCAAATGTC



gi|6462076|gb|AW187640.1|AW187640 BNLGHi8651 Six-day Cotton fiber Gossypium hirsutum cDNA 5', mRNA sequence

TGTCAGAAGAGAGTGAGCAC
.||||||||||| .||| ||
GCAGTCTTCTCTGGCTCTTG

EST sequence (targeted fragment marked with yellow):
CGATCGGATCTGTCAAGAACGAGCTGACGACTACAGGGGAAGCGGCTGACTGCAGTCCCTGAGCCCAGCTTGTAGCAAGCCAAAAGTTTGACTTGAACCTACTTTGCTAAGAGAAGAGAGAGAAGGGAAAGACAGACGGCAGAAAGCAATCCCTTCCAACCAGCCAACCCGCGGAGTTGAATTGAACACAACACTGACTTCGGCCAGGTTCTTCTATCAATCTCCTGGCCCTTGCTTAACTCCTTGTTCCGTAAACCGGTCGCTGGTTGATCTGATCGGACCCCGGACACGCGAGAGCCCAGCCCGTGAGGAATGCGTGGTTCCCTGGCGCTTCATGGGACGGGCGCTCACAGTCCCCCTCCCTCTAATCTAACCCTACCTTGCTCGCCCAGGCCTGCCGGCACAATAAGAGAATGAGGGAGCTAATCTGCTTGCTTGTGGCGGCGATGACCACTCGGCTAGAGCTCGCCTGTAGAGCTTCGACTGACTTGTTGTGGCTGTCGATCAGCGATGAATCGGTTCGCTCCATGCTCTTTGATAGACATCTTTCTGTTCTCGGTCTCTTCTGACGATTATCTTTGGGTCCTTATTCTGTGT



gi|78336422|gb|DT556696.1|DT556696 EST1067336 GH_TMO Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
TGAAGGGGCACTAGGCAAAACTCATGGAAATGGGTTCGGGCTCTTTGACTGAGTCGGGCGGTTCTCCCACCAACTCTTCCGCCGAGTCACTCAACGGCTTGAAGTTCGGTAAAAAGATCTACTTTGAGGATACAGCCGCTGTCGCCGCCGCCGCTGGTGGTAAAAGTGTTGGTGGTGGTGCAAACATAGGGACTCCATCCAAGTCCGGTCCAGCATCTTCAAGCTTAGCCGGGTCGTGTAGGAAAGCCAGGGTTGATGGAATGGCGCAAGGGGTTCTGCCTTCTAGGTGTCAAGTAGAAGGGTGTAAAGTGGATCTGAGTGATGCTAAGGCTTACTATTCAAGGCATAAGGTTTGTTGTATGCACTCTAAGTCATCTAAAGTCATTGTTGCTGGTCTCGAGCAAAGATTTTGTCAGCAGTGTAGCAGATTTCATCAGCTTTCTGAATTTGACAAAGGGAAACGGAGTTGTCGTAGACGGCTTGCAGGTCACAATGAGCGACGCAGGAAACCACCACCTGGATCATTATTTTCCTCTCCTTATGGCCGGCTTTCTTCCTCTATTATTGAAAATGGCAGTAGAGGTGGAAGCTTTATAGTGGATTTCTCAGCATACCCAAGGCTTTCAGGAAGGGATGCATGGCCAGCAGCTCGATCGTTAGAATGCATAACTGGAAATCGAAGCACAGCCACTGGAAGCTCATTTTCACATCCACGGCAAAACAACTCCAGCAAACCTCCTCATGACCATTTCTTGCAAGGTTCACCATGTGGGACTAGTTTCTCCAGCACTGGAATTTCTCCAGGAGAATGCTTCACAGGGTCTGGTGACTCAAGCTGTGCTCTCTCTCTTCTGTCAAATCAACCGTGGGGCTCCAGGAAC



gi|164250241|gb|EX168706.1|EX168706 F03_R-21_028 Cotton 1-14 day post anthesis Lambda Zap Express Library Gossypium hirsutum cDN

TGTCAGAAGAGAGTGAGCAC
||.|||||.||| |||.||
ACGGTCTTTTCTGACTTGTA

EST sequence (targeted fragment marked with yellow):
CTTTTGGTGGGNGCGNCTTTCNNATGCGGTCGNCACTAGTGGNTCCAANNATTCGGCACGAGTGNAAAATTGATCTGTCTTTTTGGTACAACCTAGCATCTTTATGTTGTAATAAGGCTTCTGTCCTTATGGTATCTACAAATTGGTTCTTTGAAACTGCACAAGATCATCCATGACGTGAAACAAGCACCACAAGCTTGGCATCTATAGCTAAGTAGTTTCCTTCTGCAATATGATTAAGAACTCAGTTTCATATGTTTGTTTATTCATCTATGTTAAGGATTCCATCCAAATTTTATTTCTTGGTTTATGTGAATTATCTTGTAGTCCTAGGAAGTAATAATAATAATATGATGTTCAGTCTTTTCTGGCAAAAAAAAAAAAAAAAAAAAACCNGGNNNNTNNTTNNCCNNGGNCCNANTTNGGCCCNAAAAATNNTTNANTTTTTTTTTAAAAANNTTTTTNCNTNGNANGNGGNCTTNNTNCCTTGNAAANCNTTNNTNGGGGTTNNTNCGGAAANCTGNCNNNGATNGGGGGNCANAAATTTTGGTTTTNNGTTTNTGCAGGACTTTTTGGTTNTCCCTNTCTTANCCCANAAANGATTGGGCCGGNTTTCCCTCCCCNCCCNCTNGGNGNTTTTGGGGGGGAGNCCACCAGTTTNCCGGCCTCCTGTCCCNGGGGGCCCCCCCAATNTTTCCCCCCTGTATTTAAANCCCNNTGTTCTTAATGTTNCNCCCCCAGAAAACCCCAAACACCCGTTTNTTTTGGGCAAGATANCCCCCACNGGGGGAAACGNNTTNAGGCTTTTTTTCCTTNNNTTTTGGGGNCCCGNAAANATTGGAANCCCCCCTTTATNGGNNNCCAAAACTTTGGGCTTNGGATTTNGANCTGGGGAGGGGTNTTCNGCCNGGCG



gi|78336557|gb|DT556831.1|DT556831 EST1067471 GH_TMO Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
||||| ||||||. ||||||
ACAGTATTCTCTTTCTCGTG

EST sequence (targeted fragment marked with yellow):
AAAAAAAAAAAAAGGCCCCAAAAGATACTTTCTTGTACTTCATTGTATTCAAGAATTAGAACAAAGAGATGAAATGAACTTGTAGAAGGCAAATTTTATATATATATACTTTAATTTACTTAGTTTTTTTTTCTAACTATTTAAAATGCAGAGATTGGTTATCCATCATAGACCCCCAGTGATTTCAAGAAGGAAACTTCCTTTATTGGTTGTTGTGGATTCATTTCCATCTTTTGACAGTGTTTCAAGACTACCCATTTCTCCTTCATTACAATGTAGAAAAATATCAAGGTTGAGTTCAACGTGCAGCACGTTTAGAACACGAGCATCCAATGTAGGTATTGGATCCGGGGGCTATGAAGATAAGGATGAAAGAGAAGACCAAAGTATTGTGAAGGGCCCGCGCAGTGACAGTTCATCAGAAGCTTTGAAGCCTCCGAGTCAAATTCCTTACCCTCTTTCCGTTGCTGGTGTGCTATTGGGATGTACTTTAGTATTGTCACTAATTGCCTTTGCAAAAGGTGGACCTGCATCACTCTTAGCAGCAATAGCAAAATCAGGCTTCACGGCTGCTTTCACATTGATCTTTGTTTCTGAAATCGGAGACAAGACGTTTTTCATCGCTGCACTCTTGGCCATGCAATACGAGAAAGGATTGGTTCTGTTGGGCTCGATGGGTGCTCTTTCTCTTATGACAATCTTGTCAGTAATAATCGGACGGATCTTTCAGTCAGTCCCTGCTCAATTCCAAACCACCTTGCCGCTCGGAGAATATGCAGCAATAGCTCTTCTCGTGTTCTTTGGCTTAAAATCTATAAAAGATGCATGGGACATTCCACCGACGGCAGCAAAGAAA



gi|109870990|gb|DW499966.1|DW499966 GH_TMIRS_041_G02_F Cotton Normalized Library dT primed Gossypium hirsutum cDNA, mRNA sequenc

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| |||||
ACTGTCTTCTCTCTCTCGTA

EST sequence (targeted fragment marked with yellow):
AAAACATCAGGCTATGTAAATTGTAGTGTTCATTTGTTTTGCAGGTTTCACTCACTGGAGGAGTTTGATGAAAGAAAAAGAAGTTGCAGAAAACGTCTCGAGGGACACAACCGCAGGCGAAGGAAGCCTCAGTCAATGTATCCCCTCTCTCTCATGCGTCCTGGAACCTACTTCTCTGATTACCAAGGTACTGAGTCGTTTCCTTTCGCTAGTTTGAATATACCTACTTGGCCTGGAGTTAACACGCAGGAACCCATAGTCAAGAAAGACACTCTTCAGAATCGAACCCCACCCACCTGTCAGTCGTTCAGGACCGCACCGGAAGGAAATGGGGGCAGCAGCAGTCACAATGTGTCGTGTGATAGGTTAGTATCTTGGTTTCGAGATGCAGATTATGCTCTCTCTCTTCTGTCATCTGTGCGGACACAACCTTCGTCTGGGATCGGTTCGAGTAACCTCGTACAGTCTCACTCGTTCGCCTTGGTGCATCCCTCGAGACTGAGCGTCGCGAATCCCGTTGTCGAACCGATGGGATCACTTGCTGTTGCCAATGACAGGGATGATAGGGTCCATTGTCCACAAATGGTTCACATGGATTGTGGTGAATGCTCTAAAACCCAAGACCCTCACACTCTTCCCTTTCATAGGAAATATTGAATCGAATTTAGCTTT



gi|164287562|gb|ES798382.1|ES798382 UFL_441_91 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|.||||||||||| .|.|||
ATAGTCTTCTCTC-TTTGTG

EST sequence (targeted fragment marked with yellow):
GGCACGAGGAAAGATGAATACAACAGATCACAGCTTGCAGCAGCTTCTGTTTGTTTACCAAATTCAAAAGGGTCAACAGAGGAGAGTCGCATCTCTGTGATTCCATTGCTATGTGCCATCTTTCTTGCAAACCTTGGGAGAAGAGGATGCAGCTGGCGAAGTTCTGTCTTGGAATAATGAAGCGGCATTGCTACAAGAAGAGAAAGAAGCTGAGAAGATGATTTTAGAAGCCTACGCAGCTTTACTACTTGCATTTCTTTCTACCGAAAGTAAGCGCACACGAAATGCTATTGCTGATTGTCTCCCAAATCGCAGTCTATCAATTTTGGTTCCAGTGTTGGAGAGATTTGTGGCGTTTCATTTTACATTAAACATGATTTCTCCGGAGACTCATAAAGCTGTGAACGAAGTGATCAAGTCGTGTAGGATACCATGAGAGATCTAATGAAAACTTCTGTACAGCCAACGTTCTCCGTTTTAGATTAGTGGCACCACCTGCAGATGAGGACGGGACTTGCCGGAAAACTGTATCGAGCTAAGCTCTCTGTTATAGTGCATTGACCCTTTTGTTTTTGCTTTTACCCCACCCACCATTCTTCTTTTTTCTGTGTTTCTCTCTTCTGATAATATTATTATTATTCTTTCTTGTTACCCTATNTCATCTTCTTCAAGCTGGCCTATACACACGTATATGAAAGTGAAAGCATCTTCAATTAGTTTTTCTTTGCCTTGTGTATGTAACGTATATATATAGTCCATATATATATATAGTGCCTAACTGTGTGCTTCTATATT



gi|164285224|gb|ES850935.1|ES850935 UFL_660_43 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
GGCACGAGGAAGGAGGTGGACCTTCTTCGTCGACGGGACCGAGGTGTCAAGTTGAAGGGTGTAATGTTACACTAGTCAACGCTAAAGACTATCACCGGAGACATAAAGTTTGTGAAACACACTCTAAAGCACCCAAGGTGGTGGTGTTAGGGTTAGAGCAACGCTTTTGTCAACAGTGTAGCAGGTTTCATTTAGTGTCAGAATTTGACGACTCTAAGAGGAGTTGCAGAAGGAGATTAGCAGGTCATAATGAGCGTAGAAGAAAAACCTCTCAAGATTCTGCTACCAGGAACTTTGCTCATGATAATAAACCGATGACTGGGAGTTTTCCGTATGTATCGTCGCCAACAGGACGTGCTCTCTCTCTTCTGTCATCCAAGGTTGATTCTTGGATCTCCCCGTCTAATCTCTCCACAAGATCTAGTGCCGCCTTGCGTGAGCTGATTGTGGAAAACCGTGCTGCTGTCCTCGCACGGCAGCTCGTACTCGAGCGTGACTGGCACTATATAGGTGAGCCCCAGCCTGCAGGGTCCAATCCTATATTAGTTCAGCATCACTCAATGCTTGACCCTCATCGTTGGGACTTTCCGTTCCCGGAAATCGGTGGTCAAGTCACATTAGATCTCATGCAAGCATCGAACACGGCGTTTGGAATGTTGTCGGTGAGGGGAAAAACTAAGGAAGAGGAAGAAGAGTGTTCGGAGTTGTGGCATTCCCCTGCAAGAACACATGGGATAGTTCCCCACTATTTATTCCTCATTTTCATTATTGAATTTGTTGGGCACCTGGTATAATTTAATCCCTTTCCATAATTTTGGAATTTGGAAAA



gi|78343421|gb|DT563695.1|DT563695 EST1074335 GH_TMO Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
CTTTGAAGATGTTTGCACAGGAAGCAATTCAAAGACTTCATCTTATTCAGCCACTCCTGGACCATCCCCTTCCCCGGCAAAGAGATCCAAACCCAATTGTCAGACAAAACATATTCTGCGTTGCCAAGTCGAAGGCTGTAACCTTGACCTTTCTTCGGCTAAGGATTACCATCGGAAACACAGAGTTTGTGAAAGTCACTCAAAGAGCCCTAAGGTTATTGTAGGTGGTCTGGAGCTCCGGTTCTGCCAGCAGTGTAGCAGGTTCCATGCTCTGTCAGAGTTTGATGAAAAGAAGCGAAGCTGCCGCCGGCGTCTTTCTGACCACAATGCAAGGCGCCGCAAACCACAGACAGATGCAATCCACTTCAGTGCTGCAAGGCCTTCACCAGCAGCATATGATGGGAAACAACTGATGAGTTTTGTCTGGAATAAAGTGCCATTTCTTCATAATGCTAGGCCCAATGAAAATTTTACATGGGAAGGCACATTTGTCTCCAAGTCCTCTGAAACGAAAGGTTATGCACCTACAAAAGCGGGAAATGCAAATGGACAGCTGCAACTGCCAGGCAACCAGCTGTTGAATTCCATGACATCTCGGTGTTACGATTCCAACAGATTCCTGCCTGTAAAGGGCAAGCAAAACACAGTTGAGGTTCTCAACCAAGGTGTAGAAGAATCCACAGTTGCTTCAAATATGGGTACGACGCAAGATCTTCATCGTGCTCTCTCTCTTCTGTCAAATGAGTCTTCGGTTTCTTGTGAGCCAAAACAACATGGTTCACTTGCGTACCCTGTGCATGTTAGTGGTAGCAGCATGTCTCAGCCCACGATGAATGTGATTTCGCAGAGTTTCCCCATTCCATATGCCTTATCTGAAAACTGGCAGATGGAACAAGAGCAGACTACTCAATCCC



gi|164260549|gb|ES835075.1|ES835075 UFL_005_16 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| |||||
ACTGTCTTCTCTCTCTCGTA

EST sequence (targeted fragment marked with yellow):
AAAAGGCAAAAGAGGGTCCAGTACGGTGGTCGGAGGCTCCTGTCTTCCAGCCTGTCAGGTCGAGAACTGCACTGCCGACATGACCGATGCCAAACGGTACCATCGGCGACATAAGGTGTGTGAGTTCCATGCCAAGGCTGCTGTGGTTCGAGTTGCTGGGATCCATCAACGCTTTTGTCAACAATGTAGCAGGTTCCATGAGTTATCAGAGTTTGATGAAACAAAAAGGAGCTGTCGACGACGTTTGGCCGGACACAACGAGCGCCGTCGGAAAAGTTCATCGGAATATCATGGAGAAGGCTCAAATTTTTAAAACTAGCTAAGAAGGCAAGCCTAAGATATATATATATATGCAAAAAAGAGTCAATTCAGTCTTGAATTTAATATGATCCTATGTATGGGAATGGACTTTCCCAGATGATCATGTAACTATATGGAATTTACTAATTAAGCATGCTCTCTCTCTTCTGTCAATCTTAATTTTACCCCTATAATAATGTCACCTTTTTCATGCTTGCTCTAAAAAAAAAAAAATAAAAAAAAAAAAACAAAACAACCCAAAAAAAAACTACCGAAGAA



gi|84173586|gb|DR462234.1|DR462234 CM105G10 Cotton Lambda Zap Express Library Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
CCTTTCTTCTGCTCGAGATTACCATCGGAAACACAAAGTTTGTGAAAATCATTCAAAGAGTCCGAAGGTCATTGTAAGCGGTCTGGAACGCCGGTTCTGCCAGCAATGTAGCAGGTTCCATGCGCTGTCGGAATTTGATGAAAACAAACGAAGTTGTCGTCGGCGACTTTCCGACCACAATGCGAGACGCCGCAAACCGCAAACTGAAGCTACCCACTTCAATGCTGCAAGGATTCCATCGTTGTCATCCTCGTCATATGATGGGAAGCAACGTACGAGTTTTGTCTGGAATGAAGTCCCAATTCTTCATAATGCTAGACCCAACAAGAAATTTGCATTGGAAGGCACGTTCTACTCCAAGTCCTCTCAAATGACAAGTTATACGCCTACGAAACTTGGAAACATCAATGAACAGGTGCAACTTCCAATCAATCAGCTGTCAAATCCCATCACGATGCGATGTCATGATTTCAACAAATTCCTACCCTCTAAGGGTAAGCAAAACACGGCTGAGGTTCTTTGATGGAGGTGTTGAACAATCTATGGTTGCTTCAAACACCGGCAGAGCTCAAGAACTTCCACGTGCTCTCTCTCTTCTGTCAAATGATTCCCACATTTCCAGGGAACCCAACCCCGTTCCC



gi|78351702|gb|DT571976.1|DT571976 EST1082616 GH_TMO Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
TCTGAAGCTATCCCAGATGATATAAGCAATAAGAAAGAAGTTTCTGGGATTGAGACAACCAGTACTTCCTTGACACTCGTGGCTTCTGTTGGCTCTGGTGAGCCATTACTCAGTTTAAAGCTTGGCAAACAAACATACTTTGAAGATGTTAGTACGGGAACCAATGCTACGGATTCATCTTATTCTGCCACTCTGGGAACATCATCTCCTTTATCTGCAAAGAGATCCAAACTGAATTGTCGAAGCACACATGCTGTACGTTGCCAAGTCGAAGGTTGTAACCTTGACCTTTCTTCTGCTCGAGATTACCATCGGAAACACAAAGTTTGTGAAAATCATTCAAAGAGTCCGAAGGTCATTGTAAGCGGTCTGGAACGCCGGTTCTGCCAGCAATGTAGCAGGTTCCATGCGCTGTCGGAATTTGATGAAAACAAACGAAGTTGTCGTCGGCGACTTTCCGACCACAATGCGAGACGCCGCAAACCGCAAACTGAAGCTACCCACTTCAATGCTGCAAGGATTCCATCGTTGTCATCCTCGTCATATGATGGGAAGCAACGTACGAGTTTTGTCTGGAATGAAGTCCCAATTCTTCATAATGCTAGACCCAACAAGAAATTTGCATTGGAAGGCACGTTCTACTCCAAGTCCTCTCAAATGACAAGTTATACGCCTACGAAACTTGGAAACATCAATGAACAGGTGCAACTTCCAATCAATCAGCTGTCAAATCCCATCACGATGCGATGTCATGATTTCAACAAATTCCTACCCTCTAAGGGTAAGCAAAACACGGCTGAGGTTCTTGATGGAGGTGTAGAACAATCTATGGTTGCTTCAAACACCGGCAGAGCTCAAGAACTTCAACGTGCTCTCTCTCTTCTGTCAAATGATTCC



gi|109879497|gb|DW508467.1|DW508467 GH_TMIRS_124_G06_R Cotton Normalized Library dT primed Gossypium hirsutum cDNA, mRNA sequenc

TGTCAGAAGAGAGTGAGCAC
|| |||||||||||||||||
ACTGTCTTCTCTCACTCGTG

EST sequence (targeted fragment marked with yellow):
AATGGAAAACAATATATAATTGACAAAATCATAACCAAAAATAACAATAAACATGATCACACACTTACTGTGCTTTTTATCTCTTCGTCAATAAAGAGAAAAGAACAAGAAGTCTACATATAAAATCCCATATGGGATTGTTTTCAACTCAATGATCTGTAGGAGAAGAAGGTGTAAAAAGTTTCCAGCAAATGTAGAAAGGAGAGGAAAAACAAGTGAAAAGAGTGAATTGTTAAGCTGACAGAAGAGAAGTGAGCACACGGCGGGCAAAGGTATGGCCTTTCATACAATCTGCCTGCGTGTGCTCACTCTCTTCTGTCAAACCTCTCCTTCCTCTCTAAATTACTCACTTTTTTTCCCCGATCTTTACCATTATTAGCTTTAAAACAAACTGCCTGCTATACTGCTGCTGCTTTAAATGAGATGATAGAGATCACACCTTCCTTTGAGAGACTCAACAAACAGATCTGAGAGATCATGCTTCAAATACGAAAGTCTGAATGAGAGAGAGTAGGTCAAAAGCTTTAGGGTTTTCTCTGCAATTATTCACCCAAACCCAAAGACCCCAAAAAGCATTCGCATTGTATTTCCTTCGATAACCATCCCT



gi|164260183|gb|ES799263.1|ES799263 UFL_560_83 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
CTTACTATTCAAGGCATAAGGTTTGTTGTATGCACTCTAAGTCATCTAAAGTCATTGTTGCTGGTCTCGAGCAAAGATTTTGTCAGCAGTGTAGCAGATTTCATCAGCTTTCTGAATTTGACAAAGGGAAACGGAGTTGTCGTAGACGCTTGCAGGTCACAATGAGCGGCGCAGGAAACCACCACCTGGATCATTATTTTCCTCTCCTTATGAAAATGGCAGTAGAGGTGGAAGCTTTATAGTGGATTTCTCAGCATACCCAAGGCTTCCTGGAAGGGATGCATGGCCAGCAGCTCGATCGTTAGAATGCATAACTGGAAATCGAAACACAGCTACTGGAAGCTCATTTTCACATCCATGGCAAAACAACTCCAACAAACCTCCTCATGACCATTTCTTGCAAGGTTCACCATGTGGGACTAGTTTCTCCAGCACTGGAATTTCTCCAGGAGAATGCTTCACAGGATCTGGTGACTCAAGCTGTGCTCTCTCTCTTCTGTCAAATCAACCGTGGGGCTCCAGGAACCAGGCTTTGAATTTTTCTGTAAATGGCGTGATAAGTACTGAAGGGTCCTCTGCGGCACAACCGACAACGCCTCATGGTGCAGTTGTGAACCCTTATTCAAATGCCTCTTTGGATTTCAATGGCAGTGACACTGTTCGCAGTTCTCACAAGATGCTGCCACACCTAGATTTGGGTCATATCCCAGACCCTGTTAACCGTCAATTCTCTAGCGACCTTGAGTTGTCTCAACAAAGCTGGAGGTCATATATGGAACTTGAGCAGTCCAGGGCAGCCTATGACGACTCCATGCAGCATATCCACTGGACGCTCTAAGTTTGTCAGTCCTGGAGGCGGTTTTCATGCCCGGGTTTTTTGGGCATGTATGGCAAATGGGGTTTTCTGGGCATCTGGTTTTATGGGTTTTAAAGTCGGAGTTGGATAAACCCCAAACTATCCCTCTTATCTGCTTGGTTAAGGTTTTAACTGGTGGAAACGAAGGGGTTCCCTGGGCCTAACAATTAATTTCGAACCATCCTTGGCTTAAATTATTTTAACAATTAATTGGGGGTTTTGGCCCTGGCCCCAAAAGAAAGGGGTTCGAAAAAGTAAGGAATCCCAGGCCCTTCTTGGGACAAAATTATTTTTTGCCCCACATTTTTTGTTAAAAAAAAAAAATACGCCGATGGGGGTGGGTGTGGCCCCCTCCCCAAAACTATCCTTCCTCTTTTGTAAAAAAAAAAAAAAAAAAAA



gi|109857658|gb|DW486637.1|DW486637 GH_RMIRS_059_E06_R Cotton Normalized Library random primed Gossypium hirsutum cDNA, mRNA seq

TGTCAGAAGAGAGTGAGCAC
||| ||||||||||||. |
ACAATCTTCTCTCACTTCTA

EST sequence (targeted fragment marked with yellow):
ATCAGGCAGAGGACGAGACAATGCACCACTTTTATTACGACCTAATGCAGACAATATAACAAGTAAAAGATCATCTCTAACATCTTCACTCTCTTCTAACACCACAATCATGATTGTTTGCATAGATAATAAAACACTTTCTGGATGATCATCCCTTACAACAGAAAAAAATGTACTGAACATTTCATTAACCAAATCATCACATTCAAGATCCAACATCACAACACATGATCTATATTTCGCAAGAGTCTCCAAAATTACAACTATCCTTCTAAATGATGGACCATCGGTATCGCTCAAACCATTGAAAGTGCCAACAATCAAGTTAAAAATATCCTTTAGAACATCATCACTATAAGGCGCTTCAGGCGCAGTTATCCGAGTTATCTCACAAATACAGGTTGCAACTAGAAGTTTCACATCCTTATCTTGATGCTTCAGCAGTTCAGGCTTGACAATTGCATTAAGCAGAGGCTGCATTGATTCCATTATTGATGATGTTGGAGACTGATCAAGCTCAGAAAGACATGTAGCAGCTTGCTCTAAGAGCTTAAGAAGAGCGTCCTTGGTAGTTGGAGGAGAGTCCAGCGTGGATCCCAGCTCCTTGAGCTGTTCTTCCAGCTTATCCGCCATTATTATCCGTAATTTCTACTCACAGGGCAAAGCAGCAGCTTCGTCTCCTTCTCTATAGCTCCCAGTCTCAGCTCAATGAGTCGCACAGTGGATACAAAGATAGATGAGAATATGAACAAAAGAACGAANAGGGGTTAGGGTTTTTCAGGCTATGGCTCGCGCTCGCTCGCTACGCCTCCGAGTTTCGGGATTCGAGGCTTTTTTCGAATCTAACACACAGACCT



gi|164336398|gb|ES799886.1|ES799886 UFL_479_26 Cotton fiber 0-10 day post anthesis Gossypium hirsutum cDNA, mRNA sequence

TGTCAGAAGAGAGTGAGCAC
|| |||||||||| ||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
TGGAGTTACGCGCGCCTGCACGTCGACACTTATGTGGATCCANAAGAATTNCGGCACGAAGCGAACCACAAGCTCACATTCTCAAATTCCTTGCTGTCAAGTTTATGGTTGCAACAAGGACCTTAGCTCTTCAAAAGATTACCACAAGAGGCACAAAGTTTGTGAGGCTCACTCTAAGACTGCCAAAGTAATTGTCAATGGAATTGAACAGCGGTTTTGTCAGCAGTGCAGCAGGTTTCATTTACTAGCGGAATTTGATGAGGGTAAGCGCAGTTGTCGTAAACGCCTATTAGGGCATAATGAGCGCAGAAGGAAGATTCATTTCAATACCTTTTCCGGAAAATCACATAAGTTGCTTCATTCATATCAAGGTACCAAGTTTCTGGGAACCTCCATACCGAAACAAACGCCTTTTGTTGTCCCTAACATATTTCAAGTTGACTTTCTTCATCCAGAAAGACATGCATACAGTAATCAATATCAGCAGGTTAAATCTGAAGAGAAACCAATGTATAGTTCTCAATCAGCAACGCCAATCACAAACGGGCAGTCGTCTTTCTTTCATATGCATGGCAGTGGGAAGCACATCTTTCTGGAACATTATCATCAGCAACTGAAGCCTTCAATGCTTCTAGTGCAGCACCAACAGTTAAAGAGTTATCTGGGGTTTCACGCTCAGACTGTGCTCTCTCTCTTCTGTCAGTTCAATCCTTGGATTTGTCAAGCCATGTGACAGGTATTCANATGACTAGGCCCTTGATCAATCAGCTGGCCATGCCATCGTAGCATGAGACATCTGCACGATGATTTATCGGAAAAAGCAGCATTTCTATTCAT