miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: TGTGGGTTTGAGATAATTGGG
species: Brassica oleracea
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|95860039|gb|DY028850.1|DY028850 47RDOAH_UP_034_H09_17AUG2004_065 O. Alba 47RDOAH Brassica oleracea cDNA 5', mRNA sequence

TGTGGGTTTGAGATAATTGGG
||||.||||||. |||..|.|
ACACTCAAACTTCATTGGCTC

EST sequence (targeted fragment marked with yellow):
CAGCCACGTGGACTCACTACAACCACCACCACTACAAGTAGCAGCAGTTCTAGCTCTTCCGGATCTGTAACCGGTCGGATTAGACATGCTCCGGTACCGGAAGATCCGGTTCGGTTACTGGTAGTCAATCCGGTTCGAGATCGGACTCGGTTACTTCAAACTCACAACAACCACTCTCCTCCTCCTCCTCTGCTACTTCTCCATCTACGGCGGCGGCGAATGTACTTCCCACCGGAAACATTTGCCCCTCCGGTAAGATCCAAATCACCGGAATGACACAAAGCCGTTCGAGAAGCGACGTTCTCGGATCCGGCACGGGAACGTACGGTCACGGAAGCATAATGCGAGGCGGAGGAGGAAGCAGTGTCTCTCCGGCGAAACCCATCTCCACCAGACAAGCACTGAGCTCGCCGGTTACTGTCGGTGGCTCCAGCAGAAGCAGTCCGGTTGCGGTAGGTTCGGATCCAGAGGAAGTGAAGAGAGTAGGCAACGAGATGTACAAGAAAGGTCTGTTCAGTGAGGCTTTGAAGCTGTACGATAAAGCAATAGCTTTATCACCGACAAACGCGGCTT



gi|308661880|gb|DK547613.1|DK547613 DK547613 full-length kale cDNA library (capsules) Brassica oleracea var. acephala cDNA clone

TGTGGGTTTGAGATAATTGGG
||||||||||||| ||| |..
ACACCCAAACTCTTTTACCTT

EST sequence (targeted fragment marked with yellow):
GTTGAAGAAACAAGAATGACACTTGCCAGTAATATTCATAGTAAGTGCCTTATTATTATACATAAGATTGCTGAATTTTTATTAGTTTCATAATCTAACACAACACCAAAGAGAGAGAAGAAGAAAATGCAGCGGTTTAAAGAACACCAAAGAAAGGGTTGTTGATGGCGGAATTGAGCTCTGTTCCAGCTGTGTTGATAACGGCTGGATCTATCTTGTCCATTGGCAAGAACAAGAACTCTCCTCCTTCCATTTTCTCAAACCCACATAGCTCCATGAACTCTATGCCTCCTCTCAGTGAACCAACCCTCTCCTGAAACGTTTGGTTGGTGAGTCTAATTTTCCGGAATTTCTCTTCATCTGGATTCTTAGCGACATTTCCCATGTATGTCAGCAATGTCTGGAACGCTCTCTTCACCTTAGCATCTTCCTCCTTGTGGGCTTGCTTAAGCGACCTCAAGCACTCTCTCATTTTCTCAGTCTTAGTGGCGGGTCTAATAGGCAATGAACTCTTCTTCTCCTCCACAACTGGCACTGAGGGTTTTGCAGCGGCAGGATCTTCCGCAGGCAATCCTAGTTTCCGCCTTCTTTCAGCCTTGTCTTCTTCAACTTTTTGACGGATCTTTTCCCTAGCCCGTCTTTCTTCTTCTTTCTCGGCTTTACGCAAAAGGATTATACGTTTTCTTTCGTTATCTTCTTCTATCCGCTTTGCTTCTAGAAGTTCCTTGCCAATCCTAAA



gi|308694967|gb|DK550865.1|DK550865 DK550865 full-length kale cDNA library (flowers) Brassica oleracea var. acephala cDNA clone

TGTGGGTTTGAGATAATTGGG
||||||||||||| ||| |..
ACACCCAAACTCTTTTACCTT

EST sequence (targeted fragment marked with yellow):
GCCAGTAATATTCATAGTTCATAATGTCTTATTATATATGATTATCGAAAGTTTAATAGTTTTATAATTCGACACAGTACAAAAGAGAGAGAGAAGAAGAAGACAATGCATCGATTTAAAGAACTCCAAAGAAAGGGTTGTTGATGGCGGAATTGAGCTCTGTTCCAGCTGAGTTGATAATGGCTGGATCTATCTTGTCCCTTGGTAAGAACAAGAACTCTCCTCCTTCCATTTTCTCAAACCCACATAGCTCCATGAACTCTATGCCTCCTCTCAGCGAACCAACCCTATCCTGAAATGTTTGGTTGGTGAGTCTAATTTTCCGGAATTTCTCTTCATCTGGATTCTTAGCGACATTTCCCAGGTAAGTCAGCAGAGTCTGGAACGCTCTCTTCACTTTAGCATCATCCTCCTTGTGGGCTTGCTTAAGCGACCTCAAGCATTCTCTCATTTGTTCAGTCTTAGTGGCGGGTCTAATAGGCAGTGAACTCTTCTTCTCCTCCACAACTGGCACTGAAGGTTTTGCAGCCGCAGGATCTTCAGCAGGCAGTCCTAGTTTCCGCCTTCTTTCAGCCTTGTCTTCTTCAACTTTTTGACGGATCTTTTCCCTAGCCCGTCTTTCTTCTTCTTTCTCGGCTTTACGCAAAAGGATTATACGTTTTCTTTCGTTATCTTCTTCCATCCGCTTTGCTTCTAGGAGTTCTTTGCCAATCCTAATTCGCTCCTTCTCTCTTTCACGCTCCATCCGTTTTTCTTCCTCTTCTTTCTTCTTCCGCGCACGTTCCTTTAGTTCTT



gi|122036432|gb|EH425297.1|EH425297 OL6968R Brassica oleracea var. alboglabra leaf cDNA library Brassica oleracea var. alboglabr

TGTGGGTTTGAGATAATTGGG
|||| |||||||| |||| |.
ACACACAAACTCTCTTAAACT

EST sequence (targeted fragment marked with yellow):
GTCCGACTCAAATTCTCTCAAACACACAAAAAAAATGGCCTCAACCGCTCTCTCAAGCGCCATCGTCGGAACATCCTTCCTCCGCCGTCAGACAGCTCCTATCAGCCTCCGTTCCCTCCCCTCAGCCAACACTCAATCCCTCTTCGGTCTCAAATCAGGCACCGCACGCGGCGGACGCGTCACCGCCATGGCTACATACAAGGTCAAGTTCATCACTCCAGAAGGTGAGCAGGAGGTTGAGTGCGACGACGACGTCTACGTCCTCGACGCTGCGGAAGAAGCCGGGATCGACTTGCCTTACTCTTGCCGTGCTGGTTCGTGCTCAAGCTGTGCCGGCAAAGTTGTGTCTGGATCTGTTGACCAGTCTGACCAGAGTTTCCTCGATGATGACCAGATTGCTGAAGGGTTTGTTCTCACTTGTGCGGCTTATCCTACCTCTGATGTTACCATTGAGACCCACAGAGAAGATGACATTGTTTAAAACAAAAGCTCGATAACATGCTTTTGATGGTTTCATAATCATTGTCTTTACATTTTGGGTTGAGTTTGTTGCTACTTAGTATAATCTATTGTTGTCTGTTGTAATTTTTCTTTTTTCTTGTTTTGGCCCACCTTTTGGTAAGATCAATGGATAACAGTGTAAACTATGATC



gi|308669012|gb|DK539787.1|DK539787 DK539787 full-length kale cDNA library (capsules) Brassica oleracea var. acephala cDNA clone

TGTGGGTTTGAGATAATTGGG
||||||||||||| ||| |..
ACACCCAAACTCTTTTACCTT

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGAAAAAACAAAAATGACACTTGCCAGTAATATTCATAATAAGTGCCTTATTATTATACATAAAATTGCTGAATTTTTATTAATTTCATAATCTAACACAACACCAAAAAAAAAAAAAAAAAAAATGCAGCGGTTTAAAAAACACCAAAAAAAGGGTTGTTGATGGGGGAATTGAGCTCTGTTCCCGCTGAATTGATAACGGCTGGATCTATCTTGTCCATTGGCAAAAACAAAAACTCTCCTCCTTCCATTTTCTCAAACCCACATAGCTCCATGAACTCTATGCCTCCTCTCAGTGAACCAACCCTCTCCTGAAACGTTTGGTTGGGGAGTCTAATTTTCCGGAATTTCTCTTCATCTGGATTCTTAACGACATTTCCCATGTATGTCAGCAATGTCTGGAACGCTCTCTTCACCTTAACATCTTCCTCCTTGTGGGCTTGCTTAAGCGACCTCAAGCACTCTCTCATTTTCTCAGTCTTAGTGGCGGGTCTAATAGGCAATGAACTCTTCTTCTCCTCCACAACTGGCACTGAGGGTTTTGCAGCGGCAGGGATCTTCCGCAGGCAATCCTAGTTTCCGCCTTCTTTCAGCCTTGTCTTCTTCAACTTTTTGACGGATCTTTTCCCTAGCCCGTCTTTCTTCTTCTTTCTCGGCTTTACGCAAAAGGATTATACGTTTTCTTTCGTTATCTCTTCTATCCGCTTTGCTTCTAGAAGTTCCTTGCCAATCCTAATCGCTCCTTCTCTCTTTCACGTTCCATCCGGATACTC



gi|308709044|gb|DK469101.1|DK469101 DK469101 full-length kale cDNA library (flowers) Brassica oleracea var. acephala cDNA clone

TGTGGGTTTGAGATAATTGGG
.|||||||||||| ||.||.
CTACCCAAACTCTAGTAGCCT

EST sequence (targeted fragment marked with yellow):
GAACAATGACAGTTCCTCCACTTCTCCTCCTCCTCACACTCCCCTCCCTCCCTTCTCTTCTCATCCACGCCGCCATCTCCGATGCTTACCCCACCATTCCCGGTACCGCTCCGATCGACGGAGGTTTCTCCGATGATCTCAAACCCATCCGCCGTGAGGTCTACGGCGAAGGCAAAATCTTCGACATCAGCCACCGCTACACGCCGGAGATGCCGGCGTGGGACTCAACGGAAGGAATCGGAAGGTTTCTATGGCTGGCCGCGAGCATGAAGAACGGGTCGCTCGCTAACAACTCCGAGATGAAGATCCCGACTCACACCGGGACCCACGTCGATTCGCCTGGACACGTGTACGATGAGTATTACGACGCTGGGTTCGATGTAGACTCGCCTGATCTCCAAGTCTTAAACGGTCCGGCGTTGTTGGTTGATGTTCCGAGGAACAAGAACATAACTGCCGAAGTGATGAAGTCTCTTAACATACCAAGAGGAGTTCGTCGTGTGCTTTTCAGAACATTGAATACTGACAGGCGTCTGATGTTCAAGAAGGAGTTTGATACAAGCTATGTCGGATTCATGAAGGATGGTGCACAATGGTTGGTAGACAATACTGACATCAAACTTGTTGGGGTTGATTATCTATCAGTAGCTGCATATGATGATCTGATTCCGCCCCACCTAGTATTCCTAAAAGGGCGAGAGACTATACTTGTGGAGGGATTGAAGCTGGATGATGTGAAAGGCAGGAGTCTACTCTGTTCATTGCTTACCTCTAAGACTTGTTGGAGCAGAACGGTCTCCAATTCGCTGCATCCTCATCAGTTGATTTCTTTCTCCAAAACTTG



gi|308611576|gb|DK539922.1|DK539922 DK539922 full-length kale cDNA library (seedlings) Brassica oleracea var. acephala cDNA clon

TGTGGGTTTGAGATAATTGGG
|||||||||||||||||||||
ACACCCAAACTCTATTAACCC

EST sequence (targeted fragment marked with yellow):
GTCTTAAGCATTGCTGCTACCTATTTGATAATGTTTTGAAATATTACATTTTCTCAGGTCATTTAGTTAATTATTGAATAATGGCAACGAGCTCTGGTACAACGAAAACGAAACCAGACAACCCAAACCTAAACAGATCAAGATCTCTTGGCAGAAAGCCAAAGCCTGCACCATCATCAGAAGCCGATGGATCTGACAAGAAGACAGCTGAGAAGCCTGTGCCCAATTATCTCAAACCCACAATCAGCTCCAGACCAGACCCGGTCAAGTTTTTGAAGAAGAACAGCGCTGCCGAAGACAAGCTTCTTCGTAGACGTTCCTTTGATCGTCCTCCTTCGTCTTTGACTTCATCAACTCCGTCATCCCATAATAAGTCTCTCAACACCTCTCCTGCACGCTCACGTGACAGACCCGTGGTTCCGAGAGAGAAGCCTGCCACTGGTCTGCGCTCTTCATCTTTCCATGGAAGCAGCAGCAGAGGCGGTAGAGGAGGCAGCACGGTGAAGTCGGCTCCTGTGGCTTCAAGAGGATCTCTAGGGGTGAAGAAGATCGGTCTGAGCGGTGGTAGTTCTTCCAAGAGCAAAAAGGAAGGTTCTGAGAATGTTCCAAAGAAACCTTTGGGTAAAAAAGAGATTACCCCGGAATCTTCTTCTCCTCCTGCGCCTGAACATAAGGATGAGGAGGAGATTGTCAAGGTCGAGACCGATGTACAAGTTTCTGAGCCGAAAGAAGAAGATAAAGATGAGGTTGTACAGCCTGAGGAACCCG



gi|150131131|gb|EE532103.1|EE532103 46RDOAG_UP_027_H08_30DEC2004_050 O. Alba 46RDOAG Brassica oleracea cDNA 5', mRNA sequence

TGTGGGTTTGAGATAATTGGG
| |||||||||.|.||||. |
AAACCCAAACTTTGTTAATGC

EST sequence (targeted fragment marked with yellow):
CCAGCTCTGAACGATCCAAGGATCGATAAGCTTCCTTATTCCATTAGGATACTTCTCGAATCAGCGATACGTAACTGTGATGAGTTTCAAGTCAAGAGCAAAGATGTTGAGAAGATTCTTGATTGGGAGAATACTTCTCCCAAACAAGTTGAGATTCCGTTTAAACCTGCTCGGGTGCTTCTTCAGGTATACACAGGCTAAGATAAGGCACATGATTGAGAATTGTTTGGAGGCCAAACATAATCAACTTGACTTCGGGATATCAATTGCTTGTTGAACCCTGTGAATTGCGGAAAGAGTGCTTATACACGTTCTTTATGAGATAATAAGTTTGTTGAAACCTGTGATTTGCTTGTATTAAGAAAAACTATATATTGATCATATAATAACATTTAGTTCGTAATTGTTTCAAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATGTC



gi|308656451|gb|DK475259.1|DK475259 DK475259 full-length kale cDNA library (leaves) Brassica oleracea var. acephala cDNA clone K

TGTGGGTTTGAGATAATTGGG
||||.||||||. |||..|.|
ACACTCAAACTTCATTGGCTC

EST sequence (targeted fragment marked with yellow):
GATCTTCACTCCTCTTCTCCTTCTGTGAACCTTCTCTCTTTAGCAACAACAAGCACTTTCTCTCTCTAGACACTTACATCCATTCTTTTGCTTCACCCTTCTAAAAATCACGAGATCCCCATCTCTTTCACTTTCCCTTCCCATGCCTTTGTTCAGAGTATCCAATCCTCCTTTCATCTCCACTCTTCGCCGTCTCCATTGACGACGCTAATACCAAGAAACCTCTATTAGAGTTCCTCTTCGATCATTCACTTGGTTCGTTTCTTCAGCGGTTTAAAAAAAATGTCACATTCAGATAAACCGGTTCCTGAATCTAACAACCGTCTCCGTGGTTCATTAACCTCCGACATCAATAAACCGGACTTTCGTGAGCTGGATCTCGGTTCGCCGGTTTCTCCGCTTCGTTCTCAGCCACGTGGACTCACTACAACCACCACCACTACAAGTAGCAGCAGTTCTAGCTCTTCCGGATCTGTAACCGGTCGGATTAGACATGCTCCGGTTACCGGAAGATCCGGTTCGGTTACTGGTAGTCAATCCGGTTCGAGATCGGACTCGGTTACTTCAAACTCACAACAACCACTCATCTCCTCCTCCTCCTCCTCTGCTACTTCTCCATCTACGGCGGCGGCGAATGTACTTCCCACCGGAAACATTTGCCCCTCCGGTAAGATCCAAATCACCGGAATGACACAAAGCCGTTCGAGAAGCGACGTTCTCGGATCCGGCACGGGAACGTACGGTCACGGAAGCATAATGCGAGGCGGAGGAGGAAGCAGTGTCTCTCCGGCGAAACCCATCTCCACCAGACAAGCACTGAGCTCGCCGGTTACTGTCGGTGGCTCCAGCAGAAGCAGTC



gi|308663870|gb|DK548479.1|DK548479 DK548479 full-length kale cDNA library (capsules) Brassica oleracea var. acephala cDNA clone

TGTGGGTTTGAGATAATTGGG
||| |||||||||.|||| ..
ACAACCAAACTCTGTTAAATT

EST sequence (targeted fragment marked with yellow):
TGCAAATTTGTCGTTCATTAAGCATAAAGAGCACATCTTAAATTGTCTCAAACCAACACAAGGTTCCCATACGGACCAAACATATCAAAAGGAGAGCAGCACTTTTAAGAACATAGTCGACTCTTTGAGTTTTTATGGGCCAAAACAGAGACACATCTTCTTTCTTGAGCTATCCAACAAATACATTGACGAACAGACCATCCAGCGTAACTTTAACTTCGAACTTGACAAGAGAGCCGAAAGCAGAAACCTTCCACTTCTTTCACCTTTGGAGTAGAATTTTAGTGAAACCCTCCAGCTCTGGGGCGATCGTATGGAGCACGACTGTGCCTCTCTCCTCCAGGACCTCCACGGCTGCCACCATCCCTGCTACGACCTCCAGAGCGATCGCCATTGCGATCAGGTCCATAGCGATCCCTACCACCATCCCTACCATACCTATCTTCCTTTGCACCATACCTGTCATCCTTTGCACTGTACCTATCGTCCTTTGAGCTGTACCTATCGTCCTTCGCACCGTACCTATCATCCTTAGCACTGTACCTATCATCCCTCGAGCTGTACCTTCCCCCACCACCTCTACCGGATTCATCAGGACACTCCCTTGCAAAATGTCCAGGCTTGCCACATTTAAAGCAATCTCCACCACCTGAACCTCGCCCACCAGAGGGACGGCTACGGTCACGGTCATACCCACGGTCACGGCTGCGGTCACCCTCATGATCCCTGCCTGACCCACCCTGAAGCGGCTGAGCTTTATCAACAGTTATAGTCCGCCCATCCAAATCCATCCCATTCATCGCTGC



gi|308692135|gb|DK546181.1|DK546181 DK546181 full-length kale cDNA library (flowers) Brassica oleracea var. acephala cDNA clone

TGTGGGTTTGAGATAATTGGG
||||||||||||| ||| |..
ACACCCAAACTCTTTTACCTT

EST sequence (targeted fragment marked with yellow):
CATAGTAAGTGCCTTATTATTATATATAAGATTGCTGAATTTTGATTAGTTTCATAATCTAACACAACTCCAAAGAGAGAGAAAAAAAAAATGCAGCGGTTTAAAGAACACCAAAGAAAGGGTTGTTGATGGCGGAATTGAGCTCTGTTCCAGCTGAGTTGATAACGGCTGGATCTATCTTGTCCCTTGGCAAGAACAAGAACTCTCCTCCTTCCATTTTCTCAAACCCACATAGCTCCATGAACTCTATGCCTCCTCTCAGTGAACCAACCCTCTCCTGAAACGTTTGGTTGGTGAGTCTAATTTTCCGGAATTTCTCTTCATCTGGATTCTTAGCGACATTTCCCATGTATGTCAGCAATGTCTGGAACGCTCTCTTCACCTTAGCATCTTCCTCCTTGTGGGCTTGCTTAAGCGACCTCAAGCACTCTCTCATTTTCTCAGTCTTAGTGGCGGGTCTAATAGGCAATGAACTCTTCTTCTCCTCCACAACTGGCACTGAGGGTTTTGCAGCGGCAGGATCTTCCGCAGGCAATCCTAGTTTCCGCCTTCTTTCAGCCTTGTCTTCTTCAACTTTTTGACGGATCTTTTCCCTAGCCCGTCTTTCTTCTTCTTTCTCGGCTTTACGCAAAAGGATTATACGTTTTCTTTCGTTATCTTCTTCTATCCGCTTTGCTTCTAGAAGTTCCTTGCCAATCCTAATTCGCTCCTTCTCTCTTTCACGTTCCATCCGTTTTTCTTCCTCTTCTTTCTTCTTCCGTGCACGTTCCCTTAGTTCTTGTGCTTTTATCTTC



gi|308677149|gb|DK492923.1|DK492923 DK492923 full-length kale cDNA library (capsules) Brassica oleracea var. acephala cDNA clone

TGTGGGTTTGAGATAATTGGG
..|||||||||||.||.||.
GTACCCAAACTCTGTTGACTA

EST sequence (targeted fragment marked with yellow):
AACAAGCGCTAGATAACCAAATCAGAGAAACGGAGCAAAGATTAAGAGATCTGAGCGAAAATGAAAAGAATCAGAAATGGCTTTTTGTAACTGAAGAGGACATCAAGAGTTTGCCCGGTTTCCAGAACCAGACACTGATAGCCGTCAAAGCTCCTCATGGCACAACTTTGGAAGTCCCTGATCCAGATGAGGCGGTTGGCCACCCTCAAAGGAGATACAGGATCATCCTCAGAAGTACAATGGGTCCTATTGACGTATACCTTGTTAGTGAATTTGAGGAGAAGTTTGAAGACACAAATGGGCCTGCTGCACCACCACCACCATGCTTGCCTATTGCTTCTTGCTCGGGATCTACAGAAAACCGTAACATAGAAGCCTTAACTGTTGATAACACAGGAACTGCCGTTGAGGATCAGTTGTCTCAAACCCATGCAAATGCTCAACCCGGCGAGACCTCTGATCTTAACTATTTGGAGGAGCAGGTAGGTGGAATGCTTAAGATTACTCCCTCTGAAGTCGAAAATGATGATACGGATTACTGGCTTCTCTCCAATGCGGACATTAGCATGACAGATATTTGGAAAACAGACTGTGGTATCGATTGAGACTATGGAATAGCTGATGTGAGTACTCCACCACCGATAATCGGTGACATATCCCCAACCGCTATTGACTCAATCCCGAGATGATGCAAGAAGGTCAAACATGCATCTCATCCCAAATGCTTTTCTTTCTCAACAACTCTTGGCGCCCCAATCAAATCTAAAGAGGGACCAAATACAGAAACGTGTATGGACCAATGGCAGTGCGTACCATAAATTATACGATTAAAATTATGATCTCATTTATCGCTGGAGTGGTGTAACGAGTGACAATGTAGA



gi|308663808|gb|DK544634.1|DK544634 DK544634 full-length kale cDNA library (capsules) Brassica oleracea var. acephala cDNA clone

TGTGGGTTTGAGATAATTGGG
|||||.||||| ||||||||
ACACCTAAACTGAATTAACCC

EST sequence (targeted fragment marked with yellow):
AAAAATCCTTACAAACATCCCAATTAAGTCAAATCCACAAAATATCCAATCATTCAGTTGTAAATAAATAATAAGTTAGAAACTTAACAGCTACACAAGTTGAAGGAAGAACCTGAAACAACATGAGCCTAACTGATTACATAATACAGCATAGTACAAACAAATCCCATTTTGTAAATCACCCCTAAGATTAAACACATGAAGATTAAGTTAAACCTGTCACAGTTTTTTCTCTTAATTTTCATATGTGATATGCAGAAATGAGAAGAAAGACAACTAATCCTTTCACTCTTCCTCTTCCTTCTTTTTCTCAACCTTCATCCTCTGTGTTTTCCTCTCTTTCATCATCAACACTCATTTCGTCAATGGCATCAGTGATGATATCCTTCACCTCTGTTTTTCTATGCATAAGATCAACTCCAAAATGGCTCCCAAGCTTCCGCAGAATATCAGATAAAGTTGCCGTATTGAAATCCACTTCCTTCAGAATTTTGGCAACCACTACGTGCATTTCTTTTCTGGTGGGTTCTCTTTTATTACCTTTCTTACTGGCTTTTCCTTGATCTTTGGATCCCTTTGCCTGTGGTTTACTTGTCTGTTTCTTGCCTTTCTCTTTAGAGGAATCATCTTTATCAACCTTTTGCTTCTTGCTTGAAGATGAAGTTGATTTAACAACTTTTAGAGAGGACTTTCGACCAGATCTTGCAGACCCTTTAAGAGATTTCTCCTCTTCAGCTGCTGGTTTCTCCTTCTTAGTTCTTTTTGACGAGTTTTTCTTACTGGTTGACTTTTTCTCCTCAG



gi|119426192|gb|DY014309.1|DY014309 48RDOAM_UP_009_F07_14AUG2004_053 O. Alba 48RDOAM Brassica oleracea cDNA 5', mRNA sequence

TGTGGGTTTGAGATAATTGGG
||||.||||||. |||..|.|
ACACTCAAACTTCATTGGCTC

EST sequence (targeted fragment marked with yellow):
GTGAACCTTCTCTCTTTAGCAACAACAAGCACTTTCTCTCTCTAGACACTTACATCCATTCTTTTGCTTCACCCTTCTAAAAATCACGAGATCCCCATCTCTTTCACTTTCCCTTCCCATGCCTTTGTTCAGAGTATCCAATCCTCCTTTCATCTCCACTCTTCGCCGTCTCCATTGACGACGCTAATACCAAGAAACCTCTATTAGAGTTCCTCTTCGATCATTCACTTGGTTCGTTTCTTCAGCGGTTTAAAAAAAATGTCACATTCAGATAAACCGGTTCCTGAATCTAACAACCGTCTCCGTGGTTCATTAACCTCCGACATCAATAAACCGGACTTTCGTGAGCTGGATCTCGGTTCGCCGGTTTCTCCGCTTCGTTCTCAGCCACGTGGACTCACTACAACCACCACCACTACAAGTAGCAGCAGTTCTAGCTCTTCCGGATCTGTAACCGGTCGGACTAGACATGCTCCGGTACCGGAAGATCCGGTTCGGTTACTGGTAGTCAATCCGGTTCCGAGATCGGACTCGGTTACTTCAAACTCACAACAACCACTCTCCTCCTCCTCCTCTGCTACTTCTCCATCTACGGCGGCGGGG



gi|308620528|gb|DK526763.1|DK526763 DK526763 full-length kale cDNA library (seedlings) Brassica oleracea var. acephala cDNA clon

TGTGGGTTTGAGATAATTGGG
|||||.||||| ||||||||
ACACCTAAACTGAATTAACCC

EST sequence (targeted fragment marked with yellow):
GATAGAGAGAGATTTTAAAAATCCTTACAAACATCCCAATTAAGTCAAATCCACAAAATATCCAATCATTCAGTTGTAAATAAATAATAAGTTAGAAACTTAACAGCTACACAAGTTGAAGGAAGAACCTGAAACAACATGAGCCTAACTGATTACATAATACAGCATAGTACAAACAAATCCCATTTTGTAAATCACCCCTAAGATTAAACACATGAAGATTAAGTTAAACCTGTCACAGTTTTTTCTCTTAATTTTCATATGTGATATGCAGAAATGAGAAGAAAGACAACTAATCCTTTCACTCTTCCTCTTCCTTCTTTTTCTCAACCTTCATCCTCTGTGTTTTCCTCTCTTTCATCATCAACACTCATTTCGTCAATGGCATCAGTGATGATATCCTTCACCTCTGTTTTTCTATGCATAAGATCAACTCCAAAATGGCTCCCAAGCTTCCGCAGAATATCAGATAAAGTTGCCGTATTGAAATCCACTTCCTTCAGAATTTTGGCAACCACTACGTGCATTTCTTTTCTGGTGGGTTCTCTTTTATTACCTTTCTTACTGGCTTTTCCTTGATCTTTGGATCCCTTTGCCTGTGGTTTACTTGTCTGTTTCTTGCCTTTCTCTTTAGAGGAATCATCTTTATCAACCTTTTGCTTCTTGCTTGAAGATGAAGTTGATTTAACAACTTTTAGAGAGGACTTTCGACCAGATCTTGCAGACCCTTTAAGAGATTTCTCCTCTTCAGCTGCTGGTTTCTCCTTCTTAGTCTTTTTGACGAGTTTTTCTTACTGGTGACTTTTCTCCTCAGCCTTATCTTTCTCATCTTCGGTTCAGTATCTTCCGCTTTGGATGGCTGGTCTT