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mature miRNA: TGTGTTCTCAGGTCACCCCT
species: Cryptomeria japonica
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|190376617|gb|BY883191.1|BY883191 BY883191 Cryptomeria japonica male strobilus Cryptomeria japonica cDNA clone CMFL015_I17 5',

TGTGTTCTCAGGTCACCCCT
..|||||.||||||| |||
GTACAAGGGTCCAGTTGGGT

EST sequence (targeted fragment marked with yellow):
GGTGTTCTGGCGAAAGAGCGTTTTAAGCATATTGAAGTTGTTTTGTGAAGTGGGTTGACCTGGGAACATGGCTTTTCTAAGCACTCGGGCTATGCTGGTTTTGGGTGTATCAATGGCGGTCGCCCTCCACTGCGCTGCTGCGGCTACTTACACCGTGGGAGGCAGCACAGGGTGGGCTGTGCCCACTGCCAATACGCTATACACCAGCTGGGCTTCCAGTAACACCTTCAAAGTTGGAGATTCACTCGTGTTCAACTTCGCTACAAATGCCCATGATGTTCGTCAAGTGACTAAGACAAATTACGATAGCTGTAATTCAACTGGTGCCTTGCAAACCTATGCAACTGGACCTGCCACTGTTAATCTCACAGCAT



gi|190369676|gb|BY894597.1|BY894597 BY894597 Cryptomeria japonica male strobilus Cryptomeria japonica cDNA clone CMFL046_N02 5',

TGTGTTCTCAGGTCACCCCT
||| ||||||||.|||| |
ACAAAAGAGTCCGGTGGTAA

EST sequence (targeted fragment marked with yellow):
GAGGGGAACGCAATACGACATCACCAAAGCATTAAATGGTGGCCTGAGAAAACAGGTGGGTTCTATCCTCGATTGATTTATTTTTTTCAATTCTATGAGAAAATTGCATTTTTTTGTATGAAATTTTATCAGGAAGAATGCCAAGCGTTCTCAATGTTGAATCTATTATTAAGCGTGGTACCAGGACTGCGCTATCATCTCTTTTATCCATCTGCGGGATTGTCTATTAGCATTTTGAAATACGATGGCTTTGGGCCAAATTGGACATCCCTTCAATGTATGGTCACATGGAACGAACTATGGTATTTTCCCTTGCAATGCACATCATTGCTTTGCAGAAACTAATTTGAATGCTAGGGGTCGCTGTTTGAGCAAAACCCATATTATGGGCATCTCTGGAAAGGGTAGAAGGACAGGATTCAATAGGACCAAATGCTCGTCTTCAGAAAGCGCTCGTGCAGAGCCTTTAGAATCCAATGGAGTAGTTGAACTGCCTCTGTT



gi|190365384|gb|BY889681.1|BY889681 BY889681 Cryptomeria japonica male strobilus Cryptomeria japonica cDNA clone CMFL033_G04 5',

TGTGTTCTCAGGTCACCCCT
|||||||.||| || ||||
ACACAAGGGTCGAGCAGGGA

EST sequence (targeted fragment marked with yellow):
GGAAAATACACAAAATATCAAATTTCTTTTGCATAACAGAACCATTATTTAGATAGAGTTTTAATTGGGTTTAGAAGGTTAGATTGGGGATTTTACATCAGTATTATGTTTGACCAACTTTCTTTAGAGTTTATTTACATTAGAGATTTAGGCATTCTTGAAGGGTTTTATTGAGAGTATCAGTTTTTCATCTTTGGAGATTTATTTACTAGTTGTTTGGGGATTTTTTGAAGGAGCTGAAACATCACTTTCGGGCACTCTTCCAACAAATATCAAATCAGTAAGAAGGGACGAGCTGGGAACACAGTAAAGATTCAAAAGCCAAAAGATCGCACTGAAAGGGCCATGGCGATGAAGATGAAGGTGGCGCAACTGCATATTGTGCTATTATTGTGTGCCTTTTTGGGAGGCAGCAGGGCGAGAAATGTGACTGAGGACGAACTACTGCAGGTAGCCTCGTCTCTCAAGATGTTTGTGGACGAGCTTCCGGACATGCCCAGGCTCCAAGGATTCGTTCCCCAGGGCCAAGATTTCAAGCCTACGGAGCTTCGCATTGGCATGTTCGAGAAAAAATGGAAATTTCACAGAGACCTTCCG



gi|29055382|gb|BP173632.1|BP173632 BP173632 Cryptomeria japonica inner bark Cryptomeria japonica cDNA clone CC0020R 3', mRNA seq

TGTGTTCTCAGGTCACCCCT
||||||||||| ||||||||
ACACAAGAGTC-AGTGGGGA

EST sequence (targeted fragment marked with yellow):
CNCANGTGNNNTACCATTCTCAACTCTTTAGGGAGAAAGGTAACTTCAGCCAGCTTGCAAAGGGTACACCCATAAGTACACCCGTAAAAGGGGTGACTGAGAACACAGCGGAGGAGTAGCCAGCAGAGCCTTTCTTTTGGCTGTATTTGTGTAATTTTTTCATTATTTTTTAATTTTGTAAGGAAGTGTGTACAATAGCCTGTGACTATTAAGTGTATTTTTTTTCTCACAGGTGTGGTGTGGGTGTTTAGTGAGTATTTGCAACTTGTTTTCTTTCTAGGGAATATGTTTGCTGGGAAAAGTTGAGAGCATAGGAATGTTTAATCAAGATGGTTTGTGTATGATTCATGAGTTAGTTTTTGAGGCTTGTAATTGTTTGTTATATTACTATATCCCTCTGAGACCTCGAGGCAGAGGATCTCTCGCTATAAAATAATGTNTAGCANCAC



gi|190385257|gb|BY895983.1|BY895983 BY895983 Cryptomeria japonica male strobilus Cryptomeria japonica cDNA clone CMFL050_L04 5',

TGTGTTCTCAGGTCACCCCT
|||||||.||| || ||| |
ACACAAGGGTCAAGGGGGTA

EST sequence (targeted fragment marked with yellow):
GCGGACCCGATTGCAGCGCTCCATGTGAATGGGGGAACTGGGAACACAATAAAAATCGCAACACCTTCTGTCGTATTTTTTTCGATTGATGTTTGGCTGTGGTTTTAGCTGCAGCAATGGGTTCTAAGGCCATGGAGAATGAGTTAGTGGAACTGTTCGAAAAGGCCAAGAAGGCCGCAGACAAGGCGGCCATAGATGAAGGCCCTGTGGAAGCCGAAGAGCAAAGATGTTTAGATGCCCTCACACTCCTAGCTTCTGTTCCTGTTACTATGGCTGTTCTCATCTCTTCTCAGGTTGGTAAGCGACTTCGAAATCTCACAAAGCATCCTCGGGAGAATATTCGTTCTGTTGCACAAGATATACTTGAAGATTGGAAGAAGGTTGTTACTTCAGAAACTATGGCAAACAATGGAAACAAAGCTTCAAAGTCTGAGAGGTCAGAGTCTATCAAGGTTGAGAAGAAACCAAAGATAGAATCAGAGTCTATCAAGGTTGAGAAAAAAGTAAAGTTAGAATCGGAGTCTATCAAGGTTGAGAAAAAAGTAAAGGTAGAATTGGAGTCTATCAAGGTTGAGAAGAAAGCAAAGGTAGAACCAGAGTTTGTCAAGGTTGAGAAAAAACTAAAGGTAGAGTCAGAATCTACTAGCATTAAGAAAGCATCTT



gi|11370712|gb|AU066363.1|AU066363 AU066363 Cryptomeria japonica inner bark Cryptomeria japonica cDNA clone CC0020, mRNA sequenc

TGTGTTCTCAGGTCACCCCT
|||||| |||| ||||||||
ACACAANAGTC-AGTGGGGA

EST sequence (targeted fragment marked with yellow):
GTAAACACCACCATTCTCAACTCTTTAGGGANAAAGGTAACTTCAGCCAGCTTGCAAAGGGTACACCCATAAGTACACCCGTAAAAGGGGTGACTGANAACACAGCGGAGGAGTAGCCAGCANANCCTTTCTTTTGGCTGTATTTGTGTAATTTTTTCATTATTTTTTAATTTTGTAAGGAAGTGTGTACAATAGCCTGTGACTATTAAGTGTATTTTTTTTCTCACAGGTGTGGTGTGGGTGTTTANTGAGTATTTGCAACTTGTTTTCTTTCTAGGGAATATGTTTGCTGGGAAAAGTTGAAANCATAGGAATGTTTAATCAANATGGTTTGTGTATGATTCATGAATTANTTTTTGAAGCTTGTTAATTGTTTGTTATATTACTATATCCCNCTGAAACCTCNANGCAAAAGGATCTTC