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mature miRNA: TGTGTTCTCAGGTCGCCCCTG
species: Malus domestica
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|117949343|gb|EG999272.1|EG999272 050711ABEA005132CT (ABEA) Royal Gala seedling leaves infected with Venturia inaequalis Malus

TGTGTTCTCAGGTCGCCCCTG
|||| |||||||||.| ||||
ACACTAGAGTCCAGTGAGGAC

EST sequence (targeted fragment marked with yellow):
CTCGAAGAGAACAAGTGTTCCAGTATATGCCAAAGCGGTAGAGGGGTTCTGCAGGAGTGACCTGAGATCACATATGGCGTTATGTGTTGCGCTTGATGTGTTCTCAGGTCGCCCCTGCGGTACCTTCTCTACCACATGAATGCACTGCTATTGATCTGAATGGACACTCATGACGGACCTGAGGTGCTACATCGAAAGGTGAGAAAGTTCTATCATGTTTCGGTGTATTGAAAAATGTAAGAAACATTACAAACTTTACTCTCGACTACATGCATCGATTGTTCTACCACTACTGTAACAGAAATTTGAATCTCATACTGTAAGTACGTGTTGGTGCTCACAAAAGAAAGTCAAATCTATTCCTACCTGGATAAATCAGTGACTAAATCAATATGTCCTTACAGCAGACTTGATGGCTAGTAGCTCTCCTTCTCCAACTTTCTGAGGATAAAACATAAGTTATCTTGGTAGACTGATGATGTTGCTTATCCAGATTGAATATTCATACTCTGAGCATTATTTGACTGTCATTTGATCTTTATACTGCCATGTTACTATTATAGTTCATGCCAGAGAATACAGTATGAATTGACCAGCAGTCTGTCGTAATCCAGTCAAGCATATTGTTCAACTATACAATTGAGAAATTTGAGAGTGAAACTACAGTAATAACTCTATTACTCTAATTATTGCACACAGTTAGAGTTTGCTTATTTATAATTACATCTTAATACGTTCTTACTCTTCAGGGGTGTTATTACTGTAGAGGGTCACCTGAGTTATACAAATGCATAGAGTCACCCTATGATATGAGTTTAATTGGATATGTTATTACTATTGCAGCAGTTTGCTATTATCCCCTATGTAATCAATAATCAAGTATACAGTCTACAATTTATCAACAGTATAACTCATCATCTAGCTCTTTTCCACTCTCCCATGTAAATGGGTCTCGAGTTTGTTTATTGTAATTCATTGATTGAAATGTTGATAGTTATGTCTGAAAATTTGAGAAGAAATTCTCTCTGAGTCTCTTCTAAAAAAAATTAGCTCAAAAAAAAAAAA



gi|91037732|gb|EB148150.1|EB148150 011022ABEA003570HT (ABEA) Royal Gala seedling leaves infected with Venturia inaequalis Malus

TGTGTTCTCAGGTCGCCCCTG
|||| |||||||||.| ||||
ACACTAGAGTCCAGTGAGGAC

EST sequence (targeted fragment marked with yellow):
TTCCACAGGAGTGACCTGAGATCACATGTAGCATTTATGTATTGCACTTGATGTGTTCTCAGGTCGCCCCTGCGGTACCTTCTCTACCATATCATTGCACTGCTGTAGATTTGAATGGACATTGACAACGGACCCGAAGTGTTACATAGAAAGGTCTCATGGCACTACATTGTTTCAGTTCCCACTTCTCTTCTCTTCCTAGTAATCCAGGAACCTTTTCTGCGACATTATGGACATCCAGCCAGAAAACCCAACAGCTCGAGGCAACATTCCAATCAGACGCCGGTTCCATGATTTGGCGAGAACCCATTTGTTCGTATTTTCGTCATCAGCATCTGTCTTCTTCAATTCCTTGCTCTTCATTTTGCGACATTAATCAGTTGTAGCTTTTTTGCCGCTTGACTTATGATTCATCTTGCTTTATATATAGATTATTTGTATTTTGAAGAAAAA



gi|226787194|gb|GO542052.1|GO542052 Mdfrjg3507F09.g1 Apple_EST_Mdfrjg Malus x domestica cDNA 5' similar to gb|AAD01605.1| copper

TGTGTTCTCAGGTCGCCCCTG
|||| ||||||| |.|||||
ACACTAGAGTCCTGTGGGGAA

EST sequence (targeted fragment marked with yellow):
CGAATTCCGTTGCTGTCGCCGTTGCTGTCGAATCTATCAAGCGCCGTTGTATTCTCCGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCTCTCAAAGGGGTGTCCTGAGATCACATCAGTTCTCAGTGGTTGCTCAATTTTGATAAAAATAATTTGATCACGTTGAAATCATGGTGAAGGGTGTTGCTGTTCTCGGCTCCAGTGAGGGTGTTAAAGGAACCATCAACTTCGTCCAGGAGGGAGATGGTCCAACTACTGTGACTGGATGTATCTCTGGCCTCAAGCCTGGACTTCATGGTTTCCACGTCCATGCTTTCGGAGACACAACAAACGGTTGCTTGTCAACTGGGCCACACTTCAATCCTAATGGAAAAGAGCATGGTGCCCCTGAAGATGAGGATCGTCATGCTGGCGACCTTGGAAATGTCACTGTTGGGGATGATGGAACTGCAACCTTCACTCTTATTGACAAGCAGATACCTCTAACCGGACCACACTCTGTCATTGGTAGGGCAGTTGTTGTCCATGGCGACCCAGATGACCTGGGCAAGGGTGGACATGAGCTTAGCAAATCCACTGGAAATGCTGGTGGCAGGGTAGCT



gi|226773201|gb|GO529431.1|GO529431 Mdltx7525B23.g1 Apple_EST_Mdltx Malus x domestica cDNA 5', mRNA sequence

TGTGTTCTCAGGTCGCCCCTG
||||||||||||| |||||||
ACACAAGAGTCCAACGGGGAC

EST sequence (targeted fragment marked with yellow):
CGAATTCCGTTGCTGTCGATTTGGATACTGCAGAAGATGTTCTTCTAGTGTTCTTATAAATACAAATTATCTACATTTAAAGCAAGATGAATCATCAGTCAAGCGGCAAAGAAGCTAGAACTGATTAATGTCGCAAAATGAAGAGCAAAGAATTGAAGAAGACAGATGCTGATGTCGGAAATACAAACAAATGGGTTCTGCCCAAATCAGGGAACTCGCGTCCGATTGGAATGTTGCCTCGAGCTGTTGGGTTTTCTGGCTGCCTTCCTGTTCTGGATGTCAATAATGTCCCAGAAAAGGTTCCTGGATTACTAGGAAGAAAAGAGAAGTGGAAACTGAAACAATGTAGTGCCATGAGACCTATAAAACTCAATTTTGGTCAAAATAATGTCCAAGCAGTCGAGAGTGAGTAAGTAATGTTTCTTACATTACATTTCAATGCACCGAAACATGTTAGAACTTTCTCACCTTTCGATGTAGCACTTCGTTCCGTTATCAATGTCCATTCAAATCTACAGCAGTGCAATGATATGATAAAGAAGATACCGCAGGGGCAACCTGAGAACACATGAAGCGCAATGCACAAGCGTTACATGTGATCTCAGGTTACTCATGGAACCTCTCTACCGCTTTTGCTTATCTCTGTA



gi|91037712|gb|EB148130.1|EB148130 011022ABEA003549HT (ABEA) Royal Gala seedling leaves infected with Venturia inaequalis Malus

TGTGTTCTCAGGTCGCCCCTG
|||| |||||||||.| ||||
ACACTAGAGTCCAGTGAGGAC

EST sequence (targeted fragment marked with yellow):
TTCCACAGGAGTGACCTGAGATCACATGTAGCATTTATGTATTGCACTTGATGTGTTCTCAGGTCGCCCCTGCGGTACCTTCTCTACCATATCATTGCACTGCTGTAGATTTGAATGGACATTGACAACGGACCCGAAGTGTTACATAGAAAGGTCTCATGGCACTACATTGTTTCAGTTCCCACTTCTCTTCTCTTCCTAGTAATCCAGGAACCTTTTCTGCGACATTATGGACATCCAGCCAGAAAACCCAACAGCTCGAGGCAACATTCCAATCAGACGCCGGTTCCATGATTTGGCGAGAACCCATTTGTTCGTATTTTCGTCATCAGCATCTGTCTTCTTCAATTCCTTGCTCTTCATTTTGCGACATTAATCAGTTGTAGCTTTTTTGCCGCTTGACTTATGATTCATCTTGCTTTATATATAGATTATTTGTATTTTGAAGAAAAAAAAAAAA



gi|117949345|gb|EG999274.1|EG999274 050712ABEA003549CT (ABEA) Royal Gala seedling leaves infected with Venturia inaequalis Malus

TGTGTTCTCAGGTCGCCCCTG
|||| |||||||||.| ||||
ACACTAGAGTCCAGTGAGGAC

EST sequence (targeted fragment marked with yellow):
GTTCCACAGGAGTGACCTGAGATCACATGTAGCATTTATGTATTGCACTTGATGTGTTCTCAGGTCGCCCCTGCGGTACCTTCTCTACCATATCATTGCACTGCTGTAGATTTGAATGGACATTGACAACGGACCCGAAGTGTTACATAGAAAGGTCTCATGGCACTACATTGTTTCAGTTCCCACTTCTCTTCTCTTCCTAGTAATCCAGGAACCTTTTCTGCGACATTATGGACATCCAGCCAGAAAACCCAACAGCTCGAGGCAACATTCCAATCAGACGCCGGTTCCATGATTTGGCGAGAACCCATTTGTTCGTATTTTCGTCATCAGCATCTGTCTTCTTCAATTCCTTGCTCTTCATTTTGCGACATTAATCAGTTGTAGCTTTTTTGCCGCTTGACTTATGATTCATCTTGCTTTATATATAGATTATTTGTATTTTGAAGAAAAAAAAAAAA