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mature miRNA: TGTTTGAGCTTAGAGTGGTA
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|24064729|gb|BU873205.1|BU873205 Q052D10 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence 

TGTTTGAGCTTAGAGTGGTA
|||| |||||| .|| ||||
ACAACCTCGAACTTCTCCAT

EST sequence (targeted fragment marked with yellow):
GGATTACCAAGCGCAAAATTGAACTCGCTTAAAAATGGCCTAAAATACAGTTAATTTCAGGATTAACCCTGCCATTGCCACACCCTCCTTCTACCTCTTCAAGCTCCAACAACAACAGCAACAATAATACTATTTTGAGTCTGGTGAAGAGGAGGAATCTCAAGACATCGTTTCTCAATGGTGGAGTCGAAGCTCTAAAAATTACTAGGATTAGGACCAGTCTATCCACTCGGTCCCATTGTTATGGACAAGGTGGAGGTGCTCTTGGAACTCGTATGAACCTTTTTGATCGGCTCGCTAGAGCTGTGAAGTCATATGCAAATGCAATCCTAAGTTCCTTTGAAGATCCTGAGAA


gi|52498888|gb|CV245913.1|CV245913 WS0259.B21_L17 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS0259_L17 3', mRNA sequence 

TGTTTGAGCTTAGAGTGGTA
|.|||||||||| ||||||
ATAAACTCGAAT-TCACCAA

EST sequence (targeted fragment marked with yellow):
TTACATTAAAACAGACGACTGATATAAACATCAAAGATCCAAACTTTAAACCTAAAACAAGCACAAAAACCACTTAAGCTCAAATATCAAATTTCTCACCAAGATTCAAACTTCAACCACAAAACAAGCACAAAACACTTCAGCTCAAAAGGAATCCATAAAATAGACAAATTTATCCATTCGTTACCTAAATTCTCCAGCTCATACTTGGCTTTGCTCACTGGCTTTATTTTCTTTTGTTTCCCAGCTGAGAAATCAATCACCGGTGGAAGCGGCGCCGGAGCCGCCGACGAAATCGGCTTCGGAGACAAGGTCACGGTGACACTCCCGTCTGGACCATACTTTCTAGGCCTCCCTCTCTTCTTCTTCACTGGCATAGACCCCGCCGCGGGCGGCGGGTGGGGCGGTGGAGCTTGCGGCGGAGCTGGTGGCGCGGATCCAGGTGTTGAATTTGGGTTGTTGTCGGACCTGGGAGCGATTTGGTAATCTGAGGGAGCACCGGATCCCACCACTGTAACCCCACCACCACCACTGGTACTATTTACAGTTGTTTCCATTCTTAAACCCTTCAAAAGATTTTTTTTTAATTATTTTTTTGGACAAGTTTTCCTAAAAACCCATTTTGATTTTGTGCTCAAATCATCTCCAAATATTAAAAAGTCTCGATTTTTCTTAAAAAAAAAAAAAAAAAA


gi|73872916|gb|DT475654.1|DT475654 WS0127.BR_M18 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS0127_M18 5', mRNA sequence 

TGTTTGAGCTTAGAGTGGTA
|.||||||||||||| .||
ATAAACTCGAATCTCTTCAA

EST sequence (targeted fragment marked with yellow):
GATCTGCCTTTACTTTTCTTATAAGTCAACTATTCCAAGTTTCCAACCAATCAACCTGACGGACTCCACTTCTCCTCCTTCCCCAGTCGGGGATGACGTCGACGCCCAGCCTCCACTACCTTCTCCACCACCACATCACCAATCACAATCAGAATCAACTTCTCTAAGCTCAAATACTATTATGATCTCTGGTAGAGATCCCGATGCCCTTTTCAGCGGCGGTGGCATCAGCTTTCTTAATGGAAGCGGTGGTGCTCAATTTAGTTATGGATATTCGAGTTTTAAGGGTAAAAGAGCTTCCATGGAGGATTTCTATGAGACAAGTATTTCTGAAGTTGATGGTCAAATGGTTGCCTTCTTCGGTGTTTTTGATGGCCATGGAGGTGCTAGAACTGCAGAATATCTGAAAAATAACCTTTTCAAGAATTTAAGCAGCCACCCTGATTTTATCAGAGACACAAAGACAGCAATTGTTGAAGCATTTAGGCAGACTGATGCAGAATACCTCCATGAAGAAAAGGCCCATC


gi|38585976|gb|CK101651.1|CK101651 F106P45.5pR Populus flower cDNA library Populus trichocarpa cDNA clone F106P45 5', mRNA seque 

TGTTTGAGCTTAGAGTGGTA
. ||||||||||||.||| |
GGAAACTCGAATCTTACCTT

EST sequence (targeted fragment marked with yellow):
GCAGCTTTTAGCCTGGCAACCGAGGAACAAATAAACTAAAATCCTCCATACATGGTCTAGTAATAAACTGCACATTGTCCATAAAATGTTTCTACTACGATGTTACCAGATTTAAAACTCTTGCCTTGTACAGTTCTTTCCATTCTAAGCTCAAAGGGTGGCTAGTCTCCTCAATGTCTCTGGGGGCATAGGCAATGACAGACATCAGCACTTAAAGTTTTACTATTCACCTTCTTCTGGAAGCTTGCTGTTTGTTCCTGGCTTGATATCCCTTCAGAGACCATCCCGCAATCCTGCCATTAACCCTCAGTGGTCGGTGTTAATCCCAGAACCTGACTTTTTTCGGGAACAGAAGTAGCTGCATTAGGTGAAAAATCATCTATTCTGCAACTAACAATGTGCCATCTGAGTATCTGTTAAGCCAGAAAATTTGCTGCACTTCCCCACTTCAAAGGTACATCTATTAATTGTGTGAAGAAGGAAAGTTATTAAGCACCACTAAATTCTCCA


gi|52402203|gb|CV239486.1|CV239486 WS0232.B21_J07 PT-MB-A-13 Populus trichocarpa cDNA clone WS0232_J07 3', mRNA sequence 

TGTTTGAGCTTAGAGTGGTA
 ||||||||||||||||| |
TCAAACTCGAATCTCACCCT

EST sequence (targeted fragment marked with yellow):
GGCTCCTCCTTCAAATTACTTGCCCTAAAAAAAAAAAAAAAATGAAGTGCAGTTGCAGCAATTTTAAAAGCGTTTCATCATCTTCATCTTCTACTTCTTCTCTGCTCCTTCCACCTTCACCCTCCTCTTCTGGTCTTCCTCACTCGCTCTTTTCCCACTCTAAGCTCAAACTTCCGAAATTAAAGGCAAATCATCGTTTGGTGATCAAGAATGCTTGTGGGTTCGATAATAATGACGGGCTTCCCGTTTCTCCTAAAAAGCTCTTCATGCAAGAGGCAATTGGAGCAGAATGGTTTGTGTGATAATGGAAATACAGAATTGAATTGTAAGGAAAAAGAGTAGGGCTTCAGCCATTAAAGATCAACACTGCTGAAGAAGTTCGGTCTCTTGAGACAGAATTATCACACTTCTATTAGTTCAATGACAAAAAAAAAAAAAAAAAA