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mature miRNA: TTAGATGACCATCAGCAAAC
species: Saccharum officinarum
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|35993538|gb|CA275455.1|CA275455 SCBFSD1037C06.b SD1 Saccharum hybrid cultivar (mixed) cDNA clone SCBFSD1037C06 3', mRNA seque 

TTAGATGACCATCAGCAAAC
.|||||||||||||.|  ||
GATCTACTGGTAGTTGACTG

EST sequence (targeted fragment marked with yellow):
CGGCCCCGTCATTTGCCTGTGACACGCAGACGTTGCCACTTGGAGGCCTTCACGGCGTGCTTCGTGTTATATGCCGATAAAACGTGCACCCATTCGATGCCTTGTCGTGGACTCAGACCGCCCAGTAGAAACCTCCTCATATAGATTCAGGTTTCAAATAGAGGAGACACCTGCTCTCAGGGTTGGCCCCGATGCACAGATGGGCAGCATCCCGAGACGCTTGCGCCCTCCGATTACCAGCCTGTCGGTTTGGCATGAAGAAGATTTCGACCACGCATGGCGGAAAGTACCTCGGGGTTTGGAAGTTTCGCCCGAAAAGTTCGTAAAGAGGGTCAGTTGATGGTCATCTAGTAATATATGGTAGCCCGCCGTTTGTAACCTATCCTGTAGATTTGCTGCATAAGATGGTGTGTCGACTGTATTGTATAAGAATAGCCTGAAACATAGTTGGGACTCATCCTCTTCAGCAGACCGTTCTAACACGTTTGTACTCGGTTTTTATGATGACATTGAAGGAAACCGGCGTTTTTTGCGTGGGTGACACCTAGCTTTGGATATGAGACTTGGCAAAACCATTTCAATGAACAGATGAAATTATACTTTGTAAAAAAAAAAAAAAAAAGGGCGGCCGCTGTTCTGGAGACTAGCTACTGTACGCGNTCAAAGTAGTCAT


gi|36012056|gb|CA281796.1|CA281796 SCRFSD2021G12.g SD2 Saccharum hybrid cultivar (mixed) cDNA clone SCRFSD2021G12 5', mRNA seque 

TTAGATGACCATCAGCAAAC
||||||.||||| |||| |
AATCTATTGGTATTCGTCTA

EST sequence (targeted fragment marked with yellow):
GAGAGAGAGAAGTGGGAGAGACCTGCTAGAGGCTGCCGTGTAGGGACCGGAGAGTCAGCGACTGCGAAGGAGGGAGAGGGCGATGGCCGGGATCGGGCCGATCAGGCAGGACTGGGAGCCGGTGGTGGTGCGGAAGAAGGCACCCACCGCCGCCGCCAAGAAGGATGAGAAGGCCGTCAACGCCGCGCGCCGCGCCGGCGCAGAGATCGAGACCATGAAGAAGTACAACGCTGGTACGAACAAAGCGGCGTCCAGCGGCACATCCCTCAACACCAAGCGCCTTGACGACGACACTGAAAACCTCGCCCATGAGCGAGTTCCAAGTGACTTGAAGAAAAATCTCATGCAAGCAAGGCTCGATAAGAAGATGACCCAGGCACAGCTTGCTCAGATGATCAATGAGAAGCCACAGGTGATCCAGGAGTATGAGTCGGGCAAGGCAATCCCCAACCAGCAGATCATTGGCAAGCTCGAGAGGGCCCTGAGAACGAAGCTGCGTGGCAAGAAATAGCTTTCGTACTGAGTTATGTGTCCCCGGAGCAGCATAGGGAAGACAAGGCANACTATCTGCTTATGGTTATCTAATGGTTGGTATCTCAAGTCATGCGGACTGTTTCTGTTGTACAAGAGTGAATAACTGTGTTCTCACAA