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mature miRNA: TTATGCTTGAGTCATGTTGAT
species: Prunus persica
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|59930956|gb|AJ872925.1|AJ872925 AJ872925 Prunus persica fruit mesocarp plus epidermis 30 days after bloom Prunus persica cDNA

TTATGCTTGAGTCATGTTGAT
||||||||||| ||| ||| |
AATACGAACTCCGTAAAACGA

EST sequence (targeted fragment marked with yellow):
TTTTAATAAAAATATTCTTACTGAATTTGCCAGCATTATATTAAGTGCTGATTAACACCTGGTGGTTTTATCAGGGGAAAAAGAAAATTATCAGAACCTACAACAAATAAGGAAAAAGAAGAAAATGTGAGAATAACAAGTAGAGATCTTATTAAATCTACTGTTACATTACAGGCTACAGCCATGCACTCAAACAAGCACAGCAAAATGCCTCAAGCATAAAGAAGAATTCCCATTAAATCAGATTTAAATACAATGAAAAGGTTTCGATTAACATAACGCGACAAAAAGGCCTGCTATCCCTAATTCTATAAAGAAACCAGAGCAGCCATGTTACTTAATCAGAACCAACCACCAAAATGAGTTTGGAAGGTCCCTCGACACAATAACATTCATGAAAAAAGAGCAGAGTCAGTCCTCTCTCCCTCTTTCACCCAATCACGAGAACATGACACCGATCTTCTTAGCAAAGTCATCCCCATCTTCATCTTCCATGTATTCCTCTTCACCATCTGCATTTTCACCTTCTTCATAGTTATAGTCACCATCTTCCCCTTGTTCATAATCATAGTCTACTTCCCTCTGATCTTCAGCTTCAGGTTCCTGGTCCTCCCAATGGTCATCATCA