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mature miRNA: TTATTAAACCCGGACCGGTC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73887099|gb|DT489837.1|DT489837 WS02543.B21_F04 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02543_F04 3', mRNA sequence 

TTATTAAACCCGGACCGGTC
|||||||| |||| ||.|||
AATAATTTAGGCCAGGTCAG

EST sequence (targeted fragment marked with yellow):
CAGAACCTGATTACACAAGTCAGAGTCGACGACCGGCTGCATATCAGCGGAGGATCAAGGAAAATATAGGTAGAGCTGATGGATGAGGTCCAACCCTTCTGTTGCCTGAATTGTACCTTTCAGATTTGGACATCCGAGATTTAATTGCTGGCATGTTTGAGTATTCTGTGGAAAGCGGATATGGAGGTTGAGGTTTATTTCAGTGTCAGAGAGTTCTGCTGAATATATTAATGTATACGTAGCAATACCTAATGCATTCTTCATTCGATTCTGATCAAAGCAAATATTTACATGGGTGTGTGGAATAGAGAGCAATATTGAGTCTGTTGTCTATTAGTGAATCCAAAGGTTTTAGACATTACTATAGCAACTGTGTAGCTGGAATCCTTTGAAGATCTGGTTTTGGTTAAATCCCTGATCGGATTATCCGTATCTCGAAGCATGGCCCGGTATTTGATTAATTGGATCAACTAGCTTGATGCTGCTTGTTTGTGAATTGTGACGGATTGCTGCTTTGTTGAGGTGCCGTTAAGCTGATTGGTGACAGAAGTACTCCACCACATCATAGCGCGAGATGGCATTATATTGTTTTCATATTCTACACTGGAAGTCTTGAGAATTGTATTATAATTGTTTTTCCTTGACCTGATTGCTGTCAACAAATTATTGATTTGCGAGTTTATTAATATTAGCTTTGGTGAATTCTGAGTTGAATGTTTTTTATACCTGGTCCGGTTACATGCTAGGTTGGTTAACCTGCCGTGTTGGACTGGACCGGATTTAATAATAATTGCCGAAGACTAGCTATATTATTTTCTTTGGCAAAAGAATCATTCCAAAAAAAAAAAAAAAAAA


gi|60712148|gb|DN501958.1|DN501958 V040C02.5pR Populus male catkins cDNA library Populus trichocarpa cDNA clone V040C02 5', mRNA 

TTATTAAACCCGGACCGGTC
||||.||||||.| ||...|
AATAGTTTGGGTCAGGTTGG

EST sequence (targeted fragment marked with yellow):
AATCTATCCTAATGTTTTTTTTTTAAAAAAAAACTGAAGGCTATGGTGGTTGGACTGGGTTTGATAAATCCCAAAAGGTAATCAATGCTGGGCATCGTGCATACGAGGATGGAACAAGCTGGATCAAGAGGGCCAATGAGCATTCTGACATGGGGAGATCTAACTTGTTGATTATACAATGGGGATGGAATCTATATTCTTCTTGATGGGCACTACATCATCATTCAAAAAAGGAAGCGCTTAGTTTTCTCTCTAAGG


gi|24069387|gb|BU877863.1|BU877863 V040C02 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence 

TTATTAAACCCGGACCGGTC
||||.||||||.| ||...|
AATAGTTTGGGTCAGGTTGG

EST sequence (targeted fragment marked with yellow):
TAAGAAAAAACTGAAGGTATGGTGGTTGGACTGGGTTTGATAAATCCGAAAAGGTAATCAATGCTGGGATCGTCATAGAGGATGGAACAAGCTGGATCGGTGAAGAAATGGTACTGTAGTTTTCAAGAGGGCCAATGAGCATTCTGACATGGTGAGATCTAACTTGTTGATTATACAATGGGGATGGAATCTATATTCTTCTTGATGGGCACTAGATCATCATTCAAGAAAGGAAGCGCTTAGTTTTCTCTCTAAGGGCAGCGACAAGCTTCGACGAGATCGCTTTTTTTTTTTTTTTCTACAGGTCATGCGCTTCCATTTTCAGAATTCCAAAGTGCAGGAAATAGGTCAGAGGATATTGGATGCCGCTACAGATATTACTTATATATTCACAGGGCGTAGTTCCGGTAACATTGAAGCAGTAAGCCGAGCTTCAAATCCTGAAGATGAAAAAACTGTTTGGAGAGTTGATGATATAATTAATCTTAAATTCTTGTTGTAGATGAATCAGATACAATGATTGGA