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mature miRNA: TTCAATGGCTCGGTCAGGTTAC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52496066|gb|CV243091.1|CV243091 WS02516.B21.1_N24 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02516_N24 3', mRNA sequence

TTCAATGGCTCGGTCAGGTTAC
||||||||||| .||||.|||
TAGTTACCGAGCAGGTCCGATG

EST sequence (targeted fragment marked with yellow):
CAGTTTAGCTGATAGATTTTAATTGGGACTGTTTCTTTCCTTCCTTCTTTATCTAGGGTTTATGTGATTGCAAATTAGGATTTCCGAGAAAGAGCAGGCAAACAATTACAAATGGCTCTTCTTGTCTCTGTACCAATTTCCTCTTCATCTTCTTTATATGGCGTTTCATCAACCAGGGTCACTCTTGGTCTCCCATCTTGCAGGTTACCCATTGGTGTACTCTCAAAGTCTTTTGATGGAATTTGCTTAAGCACCCCCGTGGGAAAGGGTTTAATGACAGTTAAGGCGTTGTCTGACAGTAAGGGGACAAGTCCAGATAGTGGGCAACCTGTGTCTGAAAGTGATGATGAAGAGGAGGATGTGGTTCTTGATAAAATTCCTTTGGATTCAAAGCTGCAGCTGAAGCTTGAGCACAAGATGAAGATGAAGTTGGGGAAGAAGATAAGGCTTAGGAGTAAGAAGCTTGATCGGAAGCGAGGGATGAGGAAGAGGGGACAGTGGCCACCATCAAAAGTAAACAAGTTGAAGAATGTCTAAACTGCCTGTTGTTTTGCAGACTGAGCAGATGCAGATTCCCTACAACAGAAATTCAGCCGTGCTTGTGATGTTATTCAGTCAAAAACATCCCTTCTCTTTCTGTTCAGTTTCAACGTCCATGAAGTTGGAGAATGAAGATTATAGCCGTATAAATAATACTTGAAGGAGCCTATCAAACATAAACTTTTGGCCTCATCAGGCTCAGTTCCAACCCTTTTTTGTAGCCTGGACGAGCCATTGATTCCATGCTAGGCATTCTTGCCTGTTCTGTATATGTAAACTTTCCTTTAAGCACTTGTTAAAAGATTGTATATTCAGTACACCAAAAAAAAAAAAAAAAAA



gi|73887959|gb|DT490697.1|DT490697 WS02545.B21_L03 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02545_L03 3', mRNA sequence

TTCAATGGCTCGGTCAGGTTAC
|||||||||||| || |.|||
AAGTTACCGAGCGAGACTAATC

EST sequence (targeted fragment marked with yellow):
TCCTCGCCGATTTATTGGATCCGACATGCCGGCGCCGCCGTTTTAGTCCTCCAGATTCTCTCGTAGATCTATCGCGTCGCTATCAGGGCTGGTGTCTTTGTTAGTAGCAAGGGGGCGTTGTGGGGAGCGACAAAGAAAATATATATTGATGCCCAGATGATCAGAACTAATGTTATTGGCAGTGGAAGGAGGAGGGTTCTTCTCTTCTTCGGCTTCTGGGTATAGCAAGGGCTTGACCCTTCTTCTCTTGGGTCAGAAGCACGAAGACAAACCCATGAGAGTTACGCCGTGGAATCAGTACCAGTTGGTGGACCAAGAACCTGACTTTGACCTCCAGCTGGCTTCCTTGAAGAACCGGCTTTCCCGCGGCTGCGCTTCTTTTGTCTGCTTTGGTCGCGCTTCCGCAGGACTTGAATCCCCATCACCTCTCAAAGTAGGCCCTGCCCAACAGAAGGATGTCTTGCCAGATCCTCTTGTTGCTGACAAGGGAAAAGATCTTACAACTGAACTCGAAGGTGATAATAATGCAATAAAAGTTACTCTTAGGAGTAGTTTGAAGAAGACATCAAAAAGTATTCCAGTCCCTGTTGAGGATGCTAATCAGAGCGAGCCATTGAATGACAAAGGTAGTGATATCCCTGGTCATACAGAAAGGAGAAAAGTGCAGTGGACCGATGTTTGTGGGAGTGAGCTTGCTGAAATTAGGGAATTTGAACCAAGGTATGGGGGTACCTTGTCTTGATCTAAACTTTGATGTTATGAGATCTGCATTGAGTTTAATTCATGTTTCAAGCATATCAAGACAAAAAAAAAAAAAAAAAA



gi|73887706|gb|DT490444.1|DT490444 WS02544.B21_P19 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02544_P19 3', mRNA sequence

TTCAATGGCTCGGTCAGGTTAC
|||||||||||||| ||||||
AAGTTACCGAGCCATACCAATG

EST sequence (targeted fragment marked with yellow):
ACAAACTCTGCAAGTAGTTGCAGCATTTATTTTCATCCTGATCACTCTTTTAGTTAAACTTTGTACATGCATTACTTGTTTATGTGGTCTAAAGCAGTTTTCGTTTTTAATTGATAGGGGAATGGCAAGACATGGTTGGTTTCTCCCTGCTACTGGAGTACTGGCCACCATTATCCAATTGAAACCCGAAACTCTCACTCCATCTATTATAAACGATGCTATCATAATCATGGGTGTTGTTGCTGTGCTCATCCATGTTGTATGCAGTGCATTTGAAGATATACTTGAAGCTGGCCACTTCAAATCAATTGCTGCTGGCACCGGTCGCCTGGCCAGAGCGCTTGCCATTACTCTACTACTCATAATCTGCGTCCCAAAAATTTGGTGGTTTCTTCTTTCCGCATGGCTGTTGTTCTTTATATGGGTAACATATACTTTACGTGAGGAATTGTCAAAATTGTGTGAATCGCTATACAATGCAGTTCGCCTTCGCCACGGCCAAGAGCCAAATGAGTTGCCAGTGTAACCATACCGAGCCATTGAAGAGAATTACGGTTGATGAAAGACTGTAGTCTATATATATATATATATAATTGCGTGGTGTGATTGAAATTACAAGTGCTATTTTTACTAGTATGGCCGCAACTTGGTAACGTTATCTGTAATATATTGTTTGAATACTTGCTTTAAGAAAAAGAAAATAATGCAAAAAAAAAAAAAAAAAA