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mature miRNA: TTCATATTCGGTCAACGTTCG
species: Populus x canadensis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|56830193|gb|CX182769.1|CX182769 G09_45-45_13.ab1 leaf inoculated with Marssonia pathogen of Populus euramericana Populus x ca

TTCATATTCGGTCAACGTTCG
|.||||||||||||||.|||.
AGGTATAAGCCAGTTGTAAGT

EST sequence (targeted fragment marked with yellow):
GGCACGAGGGTTACATTTCATAAGGTATTTTTTGAGTTTATCACTTGAATGTTGACCGAATATGGATGAAAAGTTGTTTTCCGTTTCCTTTCCTCCACATTCGGTCAATGTTCCAGTGATGAGCATAAATGGTTGCAATTTTGCCAGTTTGAGCTGAAATGGCTGATCTCTAAGCTTGTGGCCCAGAAATCCTAGGTTTGCCACTCAGGAGTATCGGCTGCAGAGCCCTTTCATAACACTGACTGCCGTCTTCACAGAGAATTGAAAACTGAGGGGATAAGGAATTGAAGAAAAATGGCAGACCGAAATTGCCACCAGCACGCTGCCACTTCTTGCTCAAGCATCGCTACAGGTATGCAGTACACAGGTAGGGCAATAGTTAAAAATGCATCTAGAGCTAAACAGTAGGGGTGTGCAGCGGGCCTGAATAATTTGATGGCACTAAGGGTTTTGGAACATCAAATTCTTTGGGCCAAGGCTTTTGGAAAATAACATGGAAAAATGGCAAGTGATTCAG