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mature miRNA: TTCTTCAGGCCATCCAGGCT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73881318|gb|DT484056.1|DT484056 WS02524.B21_F05 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02524_F05 3', mRNA sequence

TTCTTCAGGCCATCCAGGCT
|||||||||||||||..||
CAGAAGTCCGGTAGGTTTGA

EST sequence (targeted fragment marked with yellow):
AAGAACAAAAGCACAGTGGAGAGTTTCTTGGAAGCTGCCGAAGCAAGAAAAAACGCAATCAATTCTGTTTTCTGGAATGACGAGATGGGACAGTGGCTCGATTACAGACTCACTAATGGCACTTTATGCAAGGAATCTGAAACATGGCAAGCTTGTAACCAAAATCAGAATGCATATGCTTCGAATTTTATTCCTTTGTGGATAGATTTGTTCCATGCAGATGCTGCTCTGGTGGAGAATGTCATGAGAAGTTTTCAAAGTTCAGGCCTGGTTCATGCTGCTGGAATTGCCACTTCTTTAATAAATTCAGGACACCAATGGGACTTTCCAAATGGCTGGGCTCCACTTCAACACATGATAGTTGAAGGTTTGCTGAGATCTGGATTGAAAGAAGCAAGGTCATTGGCAGAAGACATAGCTGTGAGGTGGATCAAAACCAACTACGTTGGATACAAGAAAACGGGTGCAATGCATGAAAAATACAATGTGCAAAAGTGTGGAGAATTTGGAGGAGGTGGATTTTACAAACCCCAGACTGGTTTTGGTTGGTCAAATGGAGTTGTATTGACATTTTTGGAGGAGTTTGGATGGCCTGAAGACCTGAGCATAGGTTGCTGATTGGTTAGGAAACTGAAGATTTGAATTTTTGATTCAACAGTTTCTTGTTGTGATCTTAGAGACTATAGGGCATTTTTTCCTCTTCAGTTCCTTGTGCTATTATTTTGATTTGATCATATGCTGATAGACCCCATAAAACACAATCAGTATTTTTTTCTTTATGTTTGTTGAAGATTGCTTCAGGACTACTACAAGTCCATAAAAGGGTTAAAAAAAAAAAAAAAAAA



gi|73891607|gb|DT494345.1|DT494345 WS01118.BR.1_C08 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS01118_C08 5', mRNA sequence

TTCTTCAGGCCATCCAGGCT
.||||||..|||.| |.|||
GAGAAGTTTGGTGGATTCGA

EST sequence (targeted fragment marked with yellow):
GCTGTTGCACAGATAAGCAAACTCAGGATTGGACAGGTCTATGCAGCATCCGTGATGTATGGATACTTCCTAAAGCGAGTTGATCAAAGGTTTCAGCTTGAGAAGACAATGAAAATCCTGCCAAATGGTGTGGATGAGACAGAGAGTGATATTCACAAAGCTGTGGGTCAGGATGTGAAACCTGGTGGTCGAGGGTTATCCTATCAAGCTCTGTCTTCCCATCCTGAAGCCATTTCTGGTGGTATCAGTCCTGGAGGATTTGGACATGGATTAAAGGCTTCACGATTGCGAAATTATGTGATGTCTTTTGATGCGGAGACACTTCAAAGGTATGCGACAATAAGATCAAAAGAGGCTGTTAGCATCATTGAGAAGCACACTGAGGCCTTGTTTGGAAGACCTGAGATTGTCATAACGCCTCAAGGGACCATTGATACTTCCAAGGATGAAGTAGTTACGATCAGCTTAGGTGGTTTGAAGAGACTTGCTTTGGAAGCTGTTACTTTTGGTTCTTTCCTCTGGGATGTAGAGAGTTATGTGGATTCGAGGTATCACTTTGTTCTCAATTAAATAATGTTCACGTGCATGCAGGCCACAAAAAAAAAAAAAAAA



gi|73876302|gb|DT479040.1|DT479040 WS02524.BR_J12 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02524_J12 5', mRNA sequence

TTCTTCAGGCCATCCAGGCT
.|||.||||||||||||||.
GAGAGGTCCGGTAGGTCCGG

EST sequence (targeted fragment marked with yellow):
GGCGCGCTCTCCATATCTGCTGCAAAATTTTCCGTTCTTGAAAGACCTAAAACCTAAAAAATCAACCAAATCTTTATTTAACACCCACCTCCTTACAAAACCCAGATTTTCTACTTGTCTCTTAATTGCCTTACTCATGGCTGAAGTTGATCAAGGCTTTGGTGAGCATGAAATTGATCATGGCGTGGACCTTTCTGCTGATCAAGGTGTTGGTCATCCTGTAGACCATGACTTGGATAATGCCACTGATCATGGCTTAGATCATGCAGTAGATCATAGCTTGGGTCATTCTGTGGATCATGGTTTGGATCATGCCACAGATCCTGGATTGGCAGTAGAGCATGACATTGATCACCCTATAGATCATCATGTGGATCCGTCTATAGACCATGATGTGGATCACCCTGTAAACCATGACATAGACCATGCTATTGATCAAGTGCCTGAGAATTTTGAGGCTTCAAAGCAAGGACAAGATGAGGATACAGTCTCTGGAGGAGGTAGTGAGAAGAGGTGGCCTGGATGGCCTGGAGAGAGTGTGTTTCGGATGTTGGTTCCTGCACAAAAAGTTGGCAGTATCATTGGCCGCAAAGGGGAGTTCATTAAAAAGATAGTCGAAGAGACAAGAGCTCGCATCAAGATACTTGATGGTCCTCCAGGGACGACAGANAGAGCTGTAATGGTATCTGCCAAGGAGGAGCCTGATTCTTCTCTTCCTCCTGCCATGGATGGCCTACTGAGGGTTCACAAACGCATTATTGATGGTTTGGAGGGTGATTCTTCTCATGCTCCACCAAGCAGT



gi|73882824|gb|DT485562.1|DT485562 WS02528.B21_I23 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02528_I23 3', mRNA sequence

TTCTTCAGGCCATCCAGGCT
|.|||||.||||| ||||||
AGGAAGTTCGGTACGTCCGA

EST sequence (targeted fragment marked with yellow):
TTCACAGTGAGCTACTAGAATCATGTATCATCAGTCTCCATATTGAATTACAGTTCCAAAAAAGGTAGAGCTAGAGGTGGTGAGGAGAAAAGGTACTATTATAATAATCATAATCATAATACATCATCAAGAAGTAGAAATGGAGGTGGTGATGAAATGATGATGATGAGTAATAGTATGAAGCCTGCATGGCTTGAAGGATTGATGGCAGAGACATTCTTTGGAGGTTGTGGGGTCCACGAGAATCGCAGAAAGAATGAGAAGAACGTCTTTTGCTTGCTCTGTTGCCTTAGTATCTGCCCTCACTGCCTTCCTTCTCATCGCTCTCATCCCCTCCTCCAGGTAAGGAGATACGTTTACCATGATGTGGTTCGCTTGGGGGATCTTGAGAAGCTCATTGACTGTTCTTATATTCAGCCCTATACAATAAACAATGCCAAGGTGATATTTCTGAATCAGAGACCACAGTCAAGGTCTTGTAAGGGTTCAGCCAACAATTGCTTCACTTGTGACAGGATACTCCAAGACCCATTCCACTTCTGTTCTCTCTCGTGCAAGGTTAATCACCTGGTGGATCAGGGGGAGGATCTCTCTGCTATTCTCTACAGGATTGATGAATCTGATTTTGCATTTTCCCAATTTGAGGGATTGAGGATGGACAGCTCCGAGATTATCGATGATGAAGGTCAAATCACCCCGAGCTCCATTCTTGAGAACCCATTACAATACAGAGGATCATCGTGCTCCAGTGACATCATGGGCAGCTCAGGAACTGCACATGAACCCGAGGTCCTGAAAAAGAAGGAAAAAAATGGCTTCCTTCAGGGGAATTTCTTCTCCTTGAGCAGCAGGCGAAAGGGATCTCCTCACAGGGCTCCTCTCTCTTAAATTAGACAAAAAAAAAAAAAAAAAA



gi|73880916|gb|DT483654.1|DT483654 WS02523.B21_D06 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02523_D06 3', mRNA sequence

TTCTTCAGGCCATCCAGGCT
..||||||.|||| ||..||
GGGAAGTCTGGTAAGTTTGA

EST sequence (targeted fragment marked with yellow):
CATGATACTTGAAATGGATCTATGCCCGGATGATTCAGATTCATTTATTAATCATGAGGTCTCAAGGTATCAGAATGAAGATGCTACGAGGACAATTATAAGGTTGGAACAGTGTGCTCGATCTTCTATGCAAAGAGCCCTTGCATCTCAAGGAGCACTTGCTATCTTGTATGGGCGCCATTTGAAGCATTATATAAAGGACACCAAGGTTATGCTCGGCAGAGCAACAGATGATATGGATGTCGATATTGACTTGAAAAGAGAAGGGCCTGCCAACAAAATATCAAGACGGCAGGCACTCATAACGATGGAAGGAGATGGTTCTTTCTTTTTAAAAAATCTTGGTAGGATTCCGATATTTTTGAACGGCAAAGAACTCATGACTGGACAGAGTATGGGTCTGAGGTCAAGTAGTTTGATTGAGATAAGGGAAATGGCATTTGTGTTTGAAGTGAATAACAAATCTGTGAGGCAGCATCTCGCGAATGTCACCAAAAACCACAAAGAAAACAATTTCAAATTTGAATGGTCAGAACAAGGGAATAACCACCGAGAAACTGATATCAAGTTTGAATGGTCTGAAGGGGTTCCGTGACTAGAGAAATGTCATCAGCAGGTTCGTTTCTACTTTTTAAGCTTCCTAGGGCAGGGAAAAAAGTTTCATAGTATTGTACCCAAATTTCTTGCTAGCATTTGTATTGTCCATGTATAGACAATGTTATGCGGTACAAATAATTTATAGATAATCCATGTTATGTATATGAATAATAGTGGAACCCAAAGGTGAGAAATTAGAATAGCTGAATAGAAGTCATAGCTGGCAAGAACTGTTATGGTAAAAAAAAAAAAAAAAAA



gi|73889240|gb|DT491978.1|DT491978 WS02549.C21_F09 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02549_F09 3', mRNA sequence

TTCTTCAGGCCATCCAGGCT
.||||||..|||.| |.|||
GAGAAGTTTGGTGGATTCGA

EST sequence (targeted fragment marked with yellow):
GCAGTCCTGGAGGATTTGGACATGGATTAAAGGCTTCACGATTGCGAAATTATGTGATGTCTTTTGATGGGGAGACACTTCAAAGGTATGCGACAATAAGATCAAAAGAGGCTGTTAGCATCATTGAGAAGCACACTGAGGCCTTGTTTGGAAGACCTGAGATTGTCATAACGCCTCAAGGGACCATTGATACTTCCAAGGATGAACTAGTTACGATCAGCTTAGGTGGTTTGAAGAGACTTGCTTTGGAAGCTGTTACTTTTGGTTCTTTCCTCTGGGATGTAGAGAGTTATGTGGATTCGAGGTATCACTTTGTTCTCAATTAAATAATGTTCACGTGCATGCAGGCCACAAAAAAAAAAAATGTAGTAGACATACAACTGACCAAAGTTAAATGCTATCTATGTATGAGTGTAAAGCTGGTGGTAACTATAAATAGATTTGGGCTGATTTTTATGTAAAAAAAAAAAAAAAAAA