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mature miRNA: TTGAGCCGTGCCAATATCACG
species: Brassica oleracea
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|308634479|gb|DK500019.1|DK500019 DK500019 full-length kale cDNA library (seedlings) Brassica oleracea var. acephala cDNA clon

TTGAGCCGTGCCAATATCACG
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AACTCGGCGCGGTTATAGGGG

EST sequence (targeted fragment marked with yellow):
GGAGGAAGAAGAAGAAATACATAAGAGAGAAGAGGAAGAAAAGAGTTTTGAGAGATTGTGAATTTGTCGAGAAAAGTCAGCAATGCCCTTACCCTTTGAAGAGTTTCAAGGGAAGGTGATTTCTTCTTATTTCTCCTCCGACATAGAGCCCACCTCTGTTCTTAGCCCTCTCGCTACATCCTCCTCCACGGTGTCTTCCTCTCACGGCGTAAACGCTGCCGTCGGAGGCGGCGGTATCTCCGGCGGCGATGAGCAATGTGGAGCGAGTGATTGGGAGCAAGTGCTTCCTGCCTCTCCCACGCATGACCATGACAACGACCAGAGCATCCTGAGGCTCATCATGATGGGAGACTCCCAAGATCCTTCTCACGAGCTCAACAACATTCTCCAGACTTCCCAATCTCCCGCCACCTTTCACCACTCCGACTACACATCGCTCGGCAGCTTCGGAGTCGTCGACGATACGACCACCACCGGCTTCGGTGGCTTAGACCACTCGGTTCCACCTCTGTTTCAGTCACAAGTGCCACCGCAGCATCCACTCCTAATCAACCAAAGCCAGACTCATTTCACCCAGAATCCGTCCCTCTTCTACGGCCATCATCAACATCAAATCCCGGCGGCGAAGCGTCTGAACCAAGGGGGGTCGGTGGGTGAGGGGATGATAACGGAGCAGCTGTTGAAGGTGGCGGAGGTGATGGAGAGAGGTGGGGAGACAAGTGTGGCACAGGGGATATTGGCGCGGCTCAATCAACAGCTCTCATCACCGGTGGGAAAGGCATTCGAAAGGGCAGCATTCTACTACATAGAGGCTCTCCACAACAGTCTCCTCCAC