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mature miRNA: TTGGCATTCTGTCCACCTCC
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|7218465|gb|AW564587.1|AW564587 LG1_296_G04.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA sequence

TTGGCATTCTGTCCACCTCC
||||||||||||| | .||
TACCGTAAGACAGGAGAGGG

EST sequence (targeted fragment marked with yellow):
GCACGAGGTGGAGGACCTGCTCCGCGCCTCCGCCGAGGTCCTTGGCAAGGGCGCCTTCGGGACGGCGTACCGGGCCGTGCTGGAGGACGCCACCACCGTCGTCGTCAAGAGGCTCAAGGAGGTGAACGCCGGCCGCCGCGACTTTGAGCAGCAGATGGAGCTGGTTGGCCGGATTCGACATGACAACGTCGTCGAGCTCCGCGCCTACTACTACTCCAAGGACGAGAAGCTGCTCGTGTACGATTACTACAGCAGGGGAAGTGTATCCAACATGCTTCATGGGAAGAGGGGAGAGGACAGAATGCCATTGGACTGGGAAACCAGATTAAAGATTGCCGTGGGAGCAGCAAGAGGAGTTGCACACATTCACACCGAGAACAACGGGAGATTTGTTCATGGCAACATCAAG



gi|45965252|gb|CN134042.1|CN134042 OX1_19_G12.g1_A002 Oxidatively-stressed leaves and roots Sorghum bicolor cDNA clone OX1_19_G1

TTGGCATTCTGTCCACCTCC
||.|||||||| || || ||
AATCGTAAGACTGGCGGCGG

EST sequence (targeted fragment marked with yellow):
CCTACTGGAAAATCCCGAGCGAATCCCGTGCCCCGCGCGGGACCGGCGTGGGCCTATACGCCTCATCTCCCTCCCTCCCTCCGCCGGGTTCCCCGACCTTTGTAAACCCTAATCAAGTTCGCACCAAATCGGTCGCACCTCAGCTCAAAATCCGAAACGATTCGAACAATCAGAACGAAGAACGATTCAGCTTCAGCTCCTGAATCCAACAGCAATGGATCGCTCCCGCTCCAAGCGCGGCTACCACTACGACCAGGACTCCCCGCCTCCCCGCTCCAAGCAGCGCTTCGACCGCCGCACAGCGGCGGCGGCGGCGGTCAGAATGCTAACTCGAGCTACCACCGCCGCGGCCCCCCCAGCGGGCGGCGGTGGCTCCGACCGCCGCGCGTTCCTGCCTCCCGACGCCGCGCCGCCCCCGCCCCCGCCGCCTCCTCCCTCGACGGCTGCGGCGGGCGGCGGCGCTGGTCCCACCACCACCACCTCCTTCCGCATCCTGTGCCCCGAGTGCAAGGCTTACGGTTTCCCACCTGGCTTCGTTGCCAAGGTCCGGGACGAGAGCGGCGCGTTCGTCACTGTGCACCCGCCCTTCGCGGGCGACTACGTCAGGATCATCGAGACGGTCG



gi|9308388|gb|BE366831.1|BE366831 PI1_41_C10.g1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

TTGGCATTCTGTCCACCTCC
||||||||||||||||||||
AACCGTAAGACAGGTGGAGG

EST sequence (targeted fragment marked with yellow):
CGGTATACTTCATGTGCGCAGAACCAGATGGTGTTTTGTCATATGATGTAAGCACCGGGATTTGGAGGCAATTTGCCATCCCACTGCCACTGCATCTGACTGACCACACACTTGCCGAGTTCCAGGGAAGGGTTATGTTAGTGGGTCTGCTGTGCAAAAATGCAGCGACCTGCGTATGCATATGGGAGTTGCAGAAGATGACTCTCCTCTGTAAGGAGGTGGACAGAATGCCAAATATCTGGTGCTTAGAGTTCTACGGTAAGCACATGAAGATGACATGCGTGGGCAACAGTGGTTTGCTCATGCTCTCCTTGAAGGCGAAGCGTATGAACCGCCTTGTTACTTACAACCTTTTCAAAAGGGAGTGGCAGAAGGTTCCTGATTGCATGCTACCATGCAGCCGCAAAAAGCAATGGATAGCATGTGGA



gi|9921327|gb|BE638221.1|BE638221 PI1_41_C10.g2_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

TTGGCATTCTGTCCACCTCC
||||||||||||||||||||
AACCGTAAGACAGGTGGAGG

EST sequence (targeted fragment marked with yellow):
TCCGGGCATCAAACTTCCACTTGCTCTAAATTTCAGGTCACAGCCTGTGGCGGTTGGCAGCACGGTATACTTCATGTGCGCAGAACCAGATGGTGTTTTGTCATATGATGTAAGCACCGGGATTTGGAGGCAATTTGCCATCCCACTGCCACTGCATCTGACTGACCACACACTTGCCGAGTTCCAGGGAAGGGTTATGCTAGTGGGTCTGCTGTGCAAAAATGCAGCGACCTGCGTCTGCATATGGGAGTTGCAGAAGATGACTCTCCTCTGGAAGGAGGTGGACAGAATGCCAAATATCTGGTGCTTAGAGTTCTACGGTAAGCACATGAAGATGACATGCCTGGGCAACAGTGGTTTGCTCATGCTCTCCTTGAAGGCGAAGCGTATGAACCGCCTTGTTACTTACAACCTTTTCAAAAGGGAGTGGCAGAAGGTTCCTGATTGCATGATACCATGCAGCCGCAAAAAGCAATGGATAGCATGTGGCACAGCGTTTGATCCGGTC



gi|7218477|gb|AW564599.1|AW564599 LG1_296_E04.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA sequence

TTGGCATTCTGTCCACCTCC
||||||||||||| | .||
TACCGTAAGACAGGAGAGGG

EST sequence (targeted fragment marked with yellow):
GCACGAGGTGGAGGACCTGCTCCGCGCCTCCGCCGAGGTCCTTGGCAAGGGCGCCTTCGGGACGGCGTACCGGGCCGTGCTGGAGGACGCCACCACCGTCGTCGTCAAGAGGCTCAAGGAGGTGAACGCCGGCCGCCGCGACTTTGAGCAGCAGATGGAGCTGGTTGGCCGGATTCGACATGACAACGTCGTCGAGCTCCGCGCCTACTACTACTCCAAGGACGAGAAGCTGCTCGTGTACGATTACTACAGCAGGGGAAGTGTATCCAACATGCTTCATGGGAAGAGGGGAGAGGACAGAATGCCATTGGACTGGGAAACCA



gi|30963473|gb|CD220039.1|CD220039 CCC1_60_G09.g1_A007 Callus culture/cell suspension Sorghum bicolor cDNA clone CCC1_60_G09_A00

TTGGCATTCTGTCCACCTCC
||||||||||||||||||||
AACCGTAAGACAGGTGGAGG

EST sequence (targeted fragment marked with yellow):
TACTGGAAGATAGTTATTCCAGCGGCATCCGTGGGTGGTCTTGTATGTTTATTGGATCTGAGCCACAGGAATTTCTACATTTGCAACCCTCTTATGCAATCACTCAAGGAGATCCCACCAAGATCAGTTCAGGGATGGTCAAGAGTCGCTGTAGGGATGGTGTTGAACGGAAGAAGTTCTAGTGATGGCTACAAAGTAATGTGGTTAGGAAATGATGGGACTTATGAAGTCTATGACTCTACAAAGAACATGTGGTCTTGCCCTGGATCTTTTCCTCCGGGCATCAAACTTCCACTTGCTCTAAATTTCAGGTCACAGCCTGTGGCGGTTGGCAGCACGGTATACTTCATGTGCGCAGAACCAGATGGTGTTTTGTCATATGATGTAAGCACCGGGATTTGGAGGCAATTTGCCATCCCACTGCCACTGCATCTGACTGACCACACACTTGCCGAGTTCCAGGGAAGGGTTATGCTAGTGGGTCTGCTGTGCAAAAATGCAGCGACCTGCGTCTGCATATGGGAGTTGCAGAAGATGACTCTCCTCTGGAAGGAGGTGGACAGAATGCCAAATATCTGGTGCTTAGAGT



gi|7535289|gb|AW671403.1|AW671403 LG1_342_F05.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA sequence

TTGGCATTCTGTCCACCTCC
|||||||.||| || ||||
AACCGTAGGACTCGTTGAGG

EST sequence (targeted fragment marked with yellow):
GCACGAGGATTTGGTGCTATGGCAAGCCTTTTTGCACCAAAGCTCCCAAGTGGCTTTGCTGCAAACCTATCTGGTGAAAAATCACCTGCTACAGATAAAATAAAGCCTATGAAAAGGGAAAGACAAGCAGTTAGACCACCTGTAGAGCATAAGTGGTCCCTTCCTGGAGTTGCTCAGGATGCCAAACCCCCAGCAATTTTTCAGCACGAGTTGATTCAGAGTTTCTCTATCAACATGTTCTGTAAGATACCTGTTAGCAAAGATCAGACCTACAAGGACCTGCGTAGTGTACTCATGAAAAGGATATTTTTGTCTGCCCTACATTTTCGAATCAATACAAGATACAAGAGGCTAAACCCTCCTTTTACGTCAGTTGAGCTAGGACCACAGTGACTCTGGAAGGGAAGGTTGCACGGACACTACTCTTACAGTAACTGCTGAACCCCAAAATTGGAGAAGTGCAATCAAAGTTGCTGTTCACGAGGTGCGAAGACTGAAAGAATTTGG



gi|9851266|gb|BE596193.1|BE596193 PI1_50_G03.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

TTGGCATTCTGTCCACCTCC
||.|||||||| || || ||
AATCGTAAGACTGGCGGCGG

EST sequence (targeted fragment marked with yellow):
GCACGAGGGCCTCATCTCCCTCCCTCCCTCCGCCGGGTTCCCCGACCTTTGTAAACCCTAATCAAGTTCGCACCAAATCGGTCGCACCTCAGCTCAAAATCCGAAACGATTCGAACAATCAGAACGAAGAACGATTCAGCTTCAGCTCCTGAATCCAACAGCAATGGATCGCTCCCGCTCCAAGCGCGGCTACCACTACGACCAGGACTCCCCGCCTCCCCGCTCCAAGCAGCGCTTCGACCGCCGCAGCGGCGGCGGCGGCGGCGGTCAGAATGCTAACTCGAGCTACCACCGCCGCGGCCCCCCCAGCGGCGGCGGTGGCTCCGACCGCCGCGCGTTCCTGCCTCCCGACGCCGCGCCGCCCCCGCCCCCGCCG