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mature miRNA: TTGGGTCTGGCATTGGCCAG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73891436|gb|DT494174.1|DT494174 WS01117.BR_K06 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS01117_K06 5', mRNA sequence 

TTGGGTCTGGCATTGGCCAG
||.||.|||||||||.  ||
AATCCGGACCGTAACTCTTC

EST sequence (targeted fragment marked with yellow):
ACTGCAGGATACTACAACCTGAAAGACATAGATGGGTACCGACCAGCAGCAAGGATTCTATCAAGGCATCATGCTATTATGAATTTCACATGTCTTGAGATGAGGGACTCAGAACAATCAGCAGAAGCTAAAAGTGGACCTCAGGAACTCGTTCAGCAGGTCCTCAGCGGAGCATGGAGAGAGAAGATTGAAGTTGCAGGTGAGAACGCACTTTCAAGATACGATGCTGAAGCTTACAATCAGATCCTTCTCAATGCCAGGCCTAATGGTGTCAACAAGTGGGGTCCTCCTAAACTGAGGATGTTTGGGGTGACATACCTTCGGTTATATGATGAACTATTTGAAGAAAAGAATTTCAACCTGTTCAAGACATTCGTCAGGAAAATGCATGCTGATCAAGATTACTGCCCAGACCCCAGCAAATATGGCCATGAAATAGGCCCGCTGGAACGATCAAACCCACCGATTCCGGTTGACGATATCATAGATGCAACGACGCCGATGAAGCCTTTCCCATGGAATAAACAAACAGATATGCCGGTTGATGGAGCTGGACAATTTGGTCTGCTTGGTGGTCTGATAAACGGGATCAAATCCATTTTCTTCAAGTGAAAACATGGAAAAATGCAGAGATATAATGAGAGTGAAGGAATCAGATTAACATTCAATGCTAATTAGGATTCCTTCTAGGGCTATGAGTGACCTTTCAATGTATCTTCTGTTTGAATAAATAATACACATTATCTAAG


gi|24065144|gb|BU873620.1|BU873620 Q057E09 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence 

TTGGGTCTGGCATTGGCCAG
|.||||||.||||||||  |
AGCCCAGATCGTAACCGTGC

EST sequence (targeted fragment marked with yellow):
GGAATGTTGACCAGTAGACAAATTTGGAACGGCTGCGTCTTCTCATCAAACCTTTGATTAATCGATCAATCAATCGCCGTAGGGAGTCCAGCCATGGCGCTATTCTCTTCAACTACAGCACCACCGTACTCTCCTCCTCTTCTCCGATTACCATATTACACTAATAAAAGAACTCTCTCTTTCTCTTCTCCTCCAAACTCTCTCTCTTTCCGATTCTCCACATCGAATCTCAGCCGTCGAAACCCTTCTCTTTCTCTTCGATCAACTTCATCCCTAGCTTCGCCGCCGTTGGAGGGGGTGGCGCCGGCTATATCGGTGACGGAGAATGCGTTGAAGCATTTGAATAGGATGAAAAATGATAGAAATGAAGATCTGTGCTTAGGAATTGGGGTTAAACAAGGTGGATGCTCTGGTATGTCTTACACTATGGATTTTGAGAATCGTGCCAATGCTAGACCCGATGATTCCATCATTGAATGTAACGGATTTGTAATAGTTTGTGATCCGAAGAGCCTCCTCTTCATATTTGGGATGCAATTGGATTACAGTGATGCTCTTATCGGTGGAGGTTTCTCTTTCAAGAACCCAAATGCTACAAAGACATGTGGCCTGTGGTAAATCCTTTGCAGCATAGAGGTGAAGTGATGTGTAGTAATATTCAACACAGTACATATACGA


gi|73875683|gb|DT478421.1|DT478421 WS02522.BR_L19 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02522_L19 5', mRNA sequence 

TTGGGTCTGGCATTGGCCAG
||||| ||||||||||  |.
AACCCCGACCGTAACCTCTT

EST sequence (targeted fragment marked with yellow):
GTTTTTTTTTTTTTTTGTCTTCCATCAAACCATCAATTTCCCTTACCATTGCATTCTCCTTCTCCAATGCCAGCCCCAAACTCTTCTCCAATTCCATAGCTTTTCCGCTCAAAACTCCCTTCTCCTTCTGCAAACTTGATACAATCTCATCAAGTTCCCCTGCTTTTCTCTCCAACTCACCAATCTTCACCTTCTGCTCACTTTTTTCACCAGCAATCTCGTTCCTTTCCTTCTCAATCCGTCCAATCTCTCTCTTCAAATCCTCAATTTCATTCACTTTCTCCACCAAATTATTTTCAGCCAAACCTCTCAATCTTTGAAGCTCCTCAATTTCTTTCTCTCCGTCCTTCATTTCCTTATCCAACAAAGCATATTGTTTATAAAGTTTCTCAATCTCTTCCTCACCCTCCCTCTCCATTTTCTCCAATTCAACCACACTATCTTTCAAACCATCAGCCCCCTTCATCCAATTATCAACATCACTCTTCAACAAATCCCTCTCGCGATAAACCCGACTCAATCTATCTCTCTCAGTCTCAACATTCATCACTAACCCCTTAACCTCACTCTCCAAGGCCCCAATTTCATTGTCCTTCCCTCTCTTCTCTCTCACCAGACCATCAACAAAACTACCCATTTCAACCATCCGGGTCTCGATAAAAACACAAAAAAA


gi|73887919|gb|DT490657.1|DT490657 WS02545.B21_J06 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02545_J06 3', mRNA sequence 

TTGGGTCTGGCATTGGCCAG
||.||.|||||||||.  ||
AATCCGGACCGTAACTTTTC

EST sequence (targeted fragment marked with yellow):
GAGAGAGAAGATTGAAGTTGCAGGTGAGAACGCACTTTCAAGATACGATGCTGAAGGTTACAATCAGATCCTTTTCAATGCCAGGCCTAATGGTGTCAACAAGTGGGGTCCTCCTAAACTGAGGATGTTTGGGGTGACATACCTTCGGTTATATGATGAACTATATGAAGAAAATAATTTCAACCTGTTCAAGACATTCGTCAGGAAAATGCATGCTGATCAAGATTTCTGCCCAGACCCCAGCAAATATGGCCATGAAATAGACCCGCTGGAACGATCAAACCCACCGATTCCGGTTGACGATATCATAGATGCAACGACGCCGAAGAAGCCTTTCCCATGGAATAAACAAACAGATATGCCTGTTGATGGAGCTGGACAATTTGGTTTGCTTGGTGGTGTGATAAACGGGATCAAATCCATTTTCTTCAAGTGAAAACATGGAAAAATGCAGAGATATAATGAGAGTGAAGGAATCAGATTAGCATTCAATGCTAATTAGGATTCCTTCTAGGGATATGAGTGAGCTTTCAATGTATCTTTTGTTTGAATAAATAATTCACATAAGGTAGAAAAAAAAAAAAAAAAAAACT


gi|24074308|gb|BU882784.1|BU882784 UM82TB11 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence 

TTGGGTCTGGCATTGGCCAG
|.|||||||||||||||  |
AGCCCAGACCGTAACCGAGC

EST sequence (targeted fragment marked with yellow):
GTCGACCACTACACAAATCCGGAACGGCTGCGTCTTCTCATCAAACCTTCGATCAATCGATCAATCAATCGCCGTAGTGAGTCCAGCCATGGCGTTATTCTCTTCAACAACAGCACCACCGTACTCTCCTCCTCTTCTCCGCATCACCAGATCACCCAATAAAAAAACTCTCTCTTTCTCTGCTGCTCCAAATTCTCTCTCTTTCCGATCCTCCACATCGAATCTCAGCCGTCGAAACCCTTCTCTTTCTCTTCGATCAACCTCATCCCTAGCTTCGCCGCCGTCGGAGGGGGTGGCGCCGGCTATATCCGTGACGGAGAATGCGTCGAAGCATCCGAATACGATACAAAATGACACAAATCAACATCTCGTGCCCACCAATCGGGGTTAAACTAGGTGGATGCTCTGGTATGTCTTACACTATGGATCCCGAGAATCGAGCCAATGCCAGACCCGATGATCCCATAATGGAATGTAACGGA


gi|73884236|gb|DT486974.1|DT486974 WS02532.B21_H02 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02532_H02 3', mRNA sequence 

TTGGGTCTGGCATTGGCCAG
|.||||||.||||||||  |
AGCCCAGATCGTAACCGAGC

EST sequence (targeted fragment marked with yellow):
GCCAGCCATGGCGTTATTCTCTTCAACAACAGCACCACCGTACTCTCCTCCTCTTCTCCGATTACCAGATTACACTAATAAAAAAACTCTCTCTTTCTCTTCTCCTCCAAATTCTCTCTCTTTCCGATTCTCCACATCGAATCTCAGCCGTCGAAACCCTTCTCTTTCTCTTCGATCAACTTCATCCCTAGCTTCGCCGCCGTTGGAGGGGGTGGCGCCGGCTATATCGGTGACGGAGAATGCGTTGAAGCATTTGAATAGGATAAAAAATGAGAGAAATGAAGATCTGTGCTTAAGAATTGGGGTTAAACAAGGTGGATGCTCTGGTATGTCTTACACTATGGATTTTGAGAATCGAGCCAATGCTAGACCCGATGATTCCATCATTGAATGTAACGGATTTGTAATTGTTTGTGATCCGAAGAGCCTCCTCTTCATATTTGGGATGCAATTGGATTACAGTGATGCTCTTATCGGTGGAGGTTTCTCTTTCAAGAACCCAAATGCTACGAAGACATGTGGCTGTGGTAAATCCTTTGCAGCATAGAGGTGAAGTGATGTGTAGTAATATTTAACACAGTACATACACGACTTTGAAATTGTGTATCTGTTGAAGATATGTCAGTTATAAAGGTTTGTGTAAACTACAAAAATTATCCCCACTTACTCTGTTGTTCATGTGGCAATCGATTTTGCCCTTAACTATATATTTCATATTTTCAATGTCACCCTTCCAACAGTTTCTGGTAAAAAAAAAAAAAAAAAA