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mature miRNA: TTGTGGCTTTCTTGCAAGTTG
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|30969053|gb|CD225619.1|CD225619 CCC1_40_E06.g1_A007 Callus culture/cell suspension Sorghum bicolor cDNA clone CCC1_40_E06_A00

TTGTGGCTTTCTTGCAAGTTG
|||||.||||||||||||| .
AACACTGAAAGAACGTTCATT

EST sequence (targeted fragment marked with yellow):
CACCCGCTCACAGGTCACAGCCCCTTGCTCATTGGATGAATGGATGGGGGGCCGGCGGACGGAAATCGCGAGAGGATGCGAAGACATTGGCGTATTGCATATGGTGGCACCAAGCATGAGGCAGGAACTGCATTACTTGCAAGAAAGTCACAAGCTCATTTCAAGATGGCTCTCTAAACTTTGGAGAATCAATTGCAGACCATACTTGTGGCTTTCTTGCAAGTTGTGCAGTTGTTGCCTCAAGCTTGCTGCCTCCCTTTGCCAGAGCTATTAGTAAAACAGGAAGTACCGTCAGTTTATGAGCTACAAAATATAAGCATAGAAAAATAATTTAGAAATGTAAGCATAATATACTAGTACATTGCTAAAGATAAATCAATACTCAATAACTTCCACATGCTTTGGATTTAAATACAAGTACATTGCTAAAGATAAATCAATAACTCAATAACTTCCACATGCTTTGAATTTTGGGTAACATACTAGCACATTGCTAAAGAAGTAAAGATAAATCAATACTCATTCCACATGCTTTGGATTCTGGTTAACATACTAGTACATTGCTAAAAGATAAATCAATACTCAATAACTTCCACATGTCAATGATATCTTTTGCAGATTAACAAGATTCAATCAT



gi|30944063|gb|CD209699.1|CD209699 HS1_54_H09.b1_A012 Heat-shocked seedlings Sorghum bicolor cDNA clone HS1_54_H09_A012 3', mRNA

TTGTGGCTTTCTTGCAAGTTG
| ||||||||||||| ||.||
ACCACCGAAAGAACGGTCGAC

EST sequence (targeted fragment marked with yellow):
AGGTAATTTTAAACTTTCTATTAATAACTAACTAAATATGTACAAAATTGGTTAAATTAACCATGTCCATTTGTATAACTCCAACTTCAGAACTATTTTGAAATTCCTTCCAGCACAGTATTATGGTTGCTTTTGGTCCTTGTTCGCAACTCCAAAATGTTTATATCCTCTTCTTGGCAAACATGTTTTACTTCTATTTTATGATCTCAGCTGCAAAATTGCAGCCACAGCTGGCAAGAAAGCCACCACTGACACGGAGCATGTTTCAGGAGAGCCATACAAAGAGCTACTCAACTCCTTCGTCAAAGTAATCTACAACGGCCAACCAACAAAGTCAGGATCTCGTAGTCAAATCTCATAAAAATGACTTGAATGCATGTTAACAAAACCCCAGCAAAGAACAAAATAGTTAGGCCTTGCATCATGACAAAAAGTGCCAAAGAAGCTTTGCAACACAAAATGCATAGAAATTCCCTGCATCCACAATTTTTTGTCATCCCATGATATAGGTAGAATGATTTAGTCGCATCAAGCAGGTATTCCTCAACATAACCACATTTGAAAACTAAAGTGATCTATTCAACTATTACTCTCCAACCAATAGCAGACTCACCTTGTACTCTCCAAAAAATAGTGGATCATGTCAGTGTACTTGAAGAAACAACCTTTCTCTGTCAAAGTCAAACTCATGTACTATTC



gi|9851777|gb|BE596704.1|BE596704 PI1_58_F04.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

TTGTGGCTTTCTTGCAAGTTG
|||||||||||||||||||||
AACACCGAAAGAACGTTCAAC

EST sequence (targeted fragment marked with yellow):
GCACGAGGGCTATCTCAACTTCGTCTTGCTTCTTCCTGTGCTCCTTTTTGTTCCTGGACGGCTAATAACTAGATCTGAGAGGCAGAAAAAAGAGGGATGGGGAGGGGGAAGATAGTGATAAGGAGGATCGACAACTCGACGAGCAGGCAGGTGACCTTCTCTAAGAGACGGAACGGGCTGCTGAAGAAGGCAAAGGAGCTCTCAATCCTCTGCGATGCGGAGGTTGGCCTCATCATCTTCTCCAGCACCGGAAGGCTCTACGAGTTCTCCAGCACCAACATGAAAGCTGTGATAGACCGATACGGAAAGGCAAAAGAGGAGCAGATTGGCACCAACAATGCAACTTCAGAACTCATGCTCTGGCAAAGGGAGGCAGCAAGCTTGAGGCAACAACTGCACAACTTGCAAGAAAGCCACAAGTATGGTCTGCAATTGATTCTGAAAAAAAAAGGATCACTCATCAATAGGAAGTTTCAATGACACATGAAAAACTTCATAGAACATAAAAAAGTGAAACGTTTCAACCAAGGATTAAGTAAATATATAGTTATTGAAGACATTAACAACATTCATTATTTCAG



gi|37709451|gb|CF760226.1|CF760226 DSAF1_57_A09.b1_A011 Drought-stressed after flowering Sorghum bicolor cDNA clone DSAF1_57_A09

TTGTGGCTTTCTTGCAAGTTG
|||||.||||||||||||| .
AACACTGAAAGAACGTTCATT

EST sequence (targeted fragment marked with yellow):
GCTCACAGGTCACAGCCCCTTGCTCATTGGATGAATGGATGGGGGGCCGGCGGACGGAAATCGCGAGAGGATGCGAAGACATTGGCGTATTGCATATGGTGGCACCAAGCATGAGGCAGGAACTGCATTACTTGCAAGAAAGTCACAAGTAAGATACCAAAGCTTGTGGTTCATTATAAGGTGTTTTAGTGCTGCCTCTTTGTTTTTTCCAACCTCTTGTTCCACAAACAGCCCTCTAGCTTGTATGTAGTCCTGTGATTCACCTTTATGGCCTTTAAGTTGACCATTATGGGCCTTCTTCCTCCTGTGTACCCTTCTGTACAGCTCAGAATTTGTAATTTTTCTGCTCATAAATCAGAGTTGTTTTATGAGGAATGAAAGCAAAAAAAAAAAAAAAAAA



gi|37753719|gb|CF769900.1|CF769900 DSBF1_4_B10.g1_A010 Drought-stressed before flowering Sorghum bicolor cDNA clone DSBF1_4_B10_

TTGTGGCTTTCTTGCAAGTTG
| ||||||||.|||||.|.|
CAAACCGAAAGGACGTTTAGC

EST sequence (targeted fragment marked with yellow):
GAAATTTTTTGGATTTTATGGTGAACTAAACAAGGCCCAAGTTTTGATAAAGTCGACGGCGGGAAACACAAAGACACGGCATATTTTGTCAACGAACGCTGTTGAAATTGAGTGACCAATAGGGGAAAAAAAAATTGTGCGGCAAGGAACTGTGTAGCGAGCACTCACCCGAAGGCTTCGTGGCCAAATATTCTGGGGAAAATGCCAGGGAAATCCTGCAAAGTTCACACAAACAGACGAAGTATACGACACTCGTGAGGCGGTGTAGGCCACGGCGGTGAGGGCCACAACAAGGTCGATGGCCGGCGATTTGCAGGAAAGCCAAACCTTCATGCGCCAGAACGTGACGTCGCTGTGGCAGAGCGACGTGCAGATGATCCTTATCCTGACCTCGTGCTCCTTGGGCGGGTCCACGACGATCTCCTCGATGGCCAGCGGCTCGCCGGCGGCCCTGCACACGGCCGCTTTGCATCGGATTGGTTTGGGGCCATGGTTCTCCATCGAGGATGCAGAAGACTCGCCGGTGGTGCCCAGTAGATGCGTTCGAAGGGCGCACGGTGTGGTTGCGAGAGACCAGAGCCAGAGCAGGCATCGA



gi|18061262|gb|BM323459.1|BM323459 PIC1_19_E07.b1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum bicolor cDNA, mRNA sequence

TTGTGGCTTTCTTGCAAGTTG
||||||||.||||||||||||
AACACCGAGAGAACGTTCAAC

EST sequence (targeted fragment marked with yellow):
GCACGAGGGCCTCTAGCGCTAGGCTACCTAGCTTCCCGTCTCTTCCCCATCAGATCAATCGTCCTGGCCGGCTATCTCAACTTCGTCTTGCTTCTTCCTGTGCTCCTTTTTGTTCCTGGACGGCTAATAACTAGATCTGAGAGGCAGAAAAAAGAGGGATGGGGAGGGGGAAGATAGTGATAAGGAGGATCGACAACTCGACGAGCAGGCAGGTGACCTTCTCTAAGAGACGGAACGGGCTGCTGAAGAAGGCAAAGGAGCTCTCAATCCTCTGCGATGCGGAGGTTGGCCTCATCATCTTCTCCAGCACCGGAAGGCTCTACGAGTTCTCCAGCACCAACATGAAAGCTGTGATAGACCGATACGGAAAGGCAAAAGAGGAGCAGATTGGCACCAACAATGCAACTTCAGAACTCATGCTCTGGCAAAGGGAGGCAGCAAGCTTGAGGCAACAACTGCACAACTTGCAAGAGAGCCACAAGCAACTAATGGGAGAGGA



gi|33110737|gb|CF074078.1|CF074078 FE1_21_B10.b1_A002 Iron-deficient seedlings Sorghum bicolor cDNA clone FE1_21_B10_A002 3', mR

TTGTGGCTTTCTTGCAAGTTG
|..|||||||||.||| |||
AGTACCGAAAGAGCGTGAAAC

EST sequence (targeted fragment marked with yellow):
TTGGAAGCCTAATCTGTTTTGGTATTTTATTCTTTGGGATGCATTACATATTGCAATACCTAAAGCTTTATTTGGTATGAAGTACTATTACATGATTTCACTATGGACAGTAAGGAAGAAAGCTGAAGTCATCCTGTATGAATATAAGATTTACACCAAACCTAAACCACCACCTACCAGACACATACTGACCATAGTCCAGCAACATGGTAAGTGGTAACCAGCTGCTGCATCAAAGTGACCACAGCAGTAAAAAGAGGAGGCACATCTGGGGAGCTTGTTGAATGCCGGTGAAGTATATGATGACCCTGATAGCCTTCAAGGCATCAACGCCGTGAATGGATAAATACTGGTTTACAAGAAGAGTTCATATCTCCGAATCCAATGAAGTTGGTTTTTCTACCACATCTGCGAATCTTATTTGTCGTGGCCTGGGAGGTTTATGATCATTCTCCTTTGGTTGCATTGTCACCCAGGGAGCTTGTTTCTTTGATTGTACTTGAATTCCAAGAAAAGATGGCAGAGGTGGCTTATTCGTCACAGATGGCAAAGTGCGAGAAAGCCATGACTC



gi|30965472|gb|CD222038.1|CD222038 CCC1_1_C06.g1_A007 Callus culture/cell suspension Sorghum bicolor cDNA clone CCC1_1_C06_A007

TTGTGGCTTTCTTGCAAGTTG
|||||.||||||||||||| .
AACACTGAAAGAACGTTCATT

EST sequence (targeted fragment marked with yellow):
CCCGCTCACAGGTCACAGCCCCTTGCTCATTGGATGAATGGATGGGGGGCCGGCGGACGGAAATCGCGAGAGGATGCGAAGACATTGGCGTATTGCATATGGTGGCACCAAGCATGAGGCAGGAACTGCATTACTTGCAAGAAAGTCACAAAATCAATTGCAGACCATACTTGTGGCTTTCTTGCAAGTTGTGCAGTTGTTGCCTCAAGCTTGCTGCCTCCCTTTGCCAGAGCTATTAGTAAAACAGGAAGTACCGTCAGTTTATGAGCTACAAAATATAAGCATAGAAAAATAATTTAGAAATGTAAGCATAATATACTAGTACATTGCTAAAGATAAATCAATACTCAATAACTTCCACATGCTTTGGATTTAAATACAAGTACATTGCTAAAGATAAATCAATAACTCAATAACTTCCACATGCTTTGAATTTTGGGTAACATACTAGCACATTGCTAAAGAAGTAAAGATAAATCAATACTCATACCACATGCTTTGGATTCTGGTTAACATACTAGTACATTGCTAAAAGATAAATCAATACTCAATAACTTCCACATGTCAATGATATCTTTTGCAGATTAACAAGATTCAATCATTTGCTGGGGTAGTCTACAGCAAATGCATTGGTTCTCTT