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mature miRNA: TTTGGATTGAAGGGAGCTCTA
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73889710|gb|DT492448.1|DT492448 WS02550.C21_M04 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02550_M04 3', mRNA sequence

TTTGGATTGAAGGGAGCTCTA
|.||||||.||..||. ||||
AGACCTAATTTTTCTTCAGAT

EST sequence (targeted fragment marked with yellow):
AAGAGGTTGTTGAAGGCCAGGCAGCTGTCCACAGATGTCATGCGCACGTAGACATGTACGAAAAGGAAGATGTTCCCCTATATCCTGCTACCGTGAATGATGAAAAATTGAATTTGCATGTTGAGAGGGTTAGTAGACTTCTTTTTAATCCAGAGAATTTCAAGATGGGTCAGAAGGTGATGGCAGCTGAGGACTTTTCCTTCTATCAAGAAGTGATTCCTGGAGTCATGCTCGATATTGGAATTAGAAATGAAAACGTGGGTGCAATTCACTCCCTACACTCACCTTACTTCTTTCTTGATGAGGACGTTCTTTCAATTGGGGCAGCATTGCACACTGCCCTGGCAGAGATTTATCTGAATGAGCATCAACAATCTGCAGCACAGTAGAGAATAAGACATCATAAGAGCATGGAAGATCATGAATAAGTTAATAGTGGTCCATGCTATAAAGTCTCTATATGTAGTGGCAGGATATGTAAAAATCAGGGGAAAAAAAAGAAGAAGTAATGAAACTCTTGATTCTAGATCACCTTCCAGTTCTGGTTAATGCAATCACCTCTTTCTGAAGAAGATTCTGGTGAGCAACCTGGATACGGGTGCAGCTATTTTGTTCTGCAGAAGTGCCAAAAGCTGCTTCAAGCTGCCTGTCCAGGTAAATGATTATGTTGCCAAAATCCTATGCGAACTTCGGTTGCCCCTCGACCTATTTTACCAAGGGATGTCCTTCCCAGAGCTACATTTGCTTGCACTCTTTTCCTGAAAGATTGAGCAATATTAATTATTCCAAAAAGTAATGAAGATTTCATGGTATATAATATAACAAAAGAAGGTGCTAAAAAAAAAAAAAAAAAA



gi|38586070|gb|CK101745.1|CK101745 F115P34.5pR Populus flower cDNA library Populus trichocarpa cDNA clone F115P34 5', mRNA seque

TTTGGATTGAAGGGAGCTCTA
| ||||||||||.||||||
TACCCTAACTTCCTTCGAGAG

EST sequence (targeted fragment marked with yellow):
GAGCTTCCCCTCCCAGGTACAAACACCTTGTGTGCCTGTGGTAATATTCTCTGGTTGGAATGTACTCTACAAACTCCCCTTTCTTTAGGGCTGTCTGCGCATGCTGATAAACCACGGTTTAAACCACCAACCAACATTGTTAATCACATTTCCCTTCTTCTTGGCCTCTTCTTTGGAGGCATATCTCCCTGTCATCATCACACCATCGGTAGAGTTATAAATCATAGTCTCCACAAAGTCTGGAACCTTGCTCGGGTTATCCTGATCTCCATCTCTGGGTGCAAAAGAGTCTATATAGGCCTGTGCAAGTTCTTTCAGATTACCCACCACAGGTTTGTAGGTCAGCCTCATGTCTTCCTTAACGGGAATAAGCTTGATCTCAGCAGAGACAAGAAGCCCAAGTGTTCCCTGAGACCAAGGGATGGCATAGAAAAGATCAGAGTATTCATTGTCCTTGGTGGCTCTAACAACCTGGCCATCTGCCAAAACAATCTCATAAGGCCACAACAGTGTCAGAGAACAAGCCATAGGATGTGAGAGCTTCCTTCAATCCCATAACCATTAAATTAGCCCCACCAACAGTGAGATCATCAAGTTCTGCAACCACTTGCAGGGAAAGCTTCATTGGGGACA



gi|38575965|gb|CK091640.1|CK091640 F115P34.3pR Populus flower cDNA library Populus trichocarpa cDNA clone F115P34 3', mRNA seque

TTTGGATTGAAGGGAGCTCTA
| ||||||||||.||||||
TACCCTAACTTCCTTCGAGAG

EST sequence (targeted fragment marked with yellow):
GCAAAAAAGTCTATATAGGCCTGGCAAGTTCTTCAGATTACCCACCACAGGTTTGTAGGTCAGCCTCATGTATTCTTAACGGGAATAGCTTGATCTCAGCAGAGACAAGAAGCCCAAGTGTTCCCTGAGACCAAGGGATGGCATAGAAAAGATCAGAGTATTCATTGTCCTTGGTGGCTCTAACAACCTGGCCATCTGCCAAAACAATCTCATAAGCCACAACAGTGTCAGAGAACAAGCCATAGATGTGAGAGCTTCCTTCAATCCCATAACCATTAATTAGCCCACCAACAGTGAGATCATCAAGTTCTGCAACCACTGCAAGGGAAAGATTCATTGGGACACTCGCCCTGCTAATCTGTCCCATATTTACAAGTGGCTCAACTCTTGCAACCATTCTCTCTCTGTCAATTTCAAGGATATTACGGAAAGATGATAAATCAACTTCAAAGTGCCGAGCCCGTTTATAGTCAACATTCCGCATTCCAACAGCAATCCAGGGTTTACGAGCAGTGCAAACAAGACCATCCTTGGATGGATTCCTCTCTTTGAGGCGTTTCACCACTTTTTTAACATTTTCATCATGTTCCTTCTGACGCTGTTTGTAGGATTTCATCTCTGATTTGACATCCCCAAGGTAAGTGAGAAAGTAAAGGGTGAAGGAGATTGGGAGAACAACAAAAATAACTAAGATCCATCTGAACTGGACAAAATAGTCTACCCACACCTTCTTCCTCTTTGGGCGCAGGGGGG



gi|52380130|gb|CV228552.1|CV228552 WS01910.B21_I01 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS01910_I01 3', mRNA sequence

TTTGGATTGAAGGGAGCTCTA
||||||||||| ||||.|||
AAACCTAACTTGACTCGGGAT

EST sequence (targeted fragment marked with yellow):
AGATTGGATTGAGGGGATTTTATGGACCATCGGCTCCCGATAGGGCTCAGTTCAATCCAAACTCATATGCTACGCAAGTTGGAACCGACTTTATCAGGAACCATCAGGTTCTTGGTGTTGATTTTGCTTCTGTTCACATGTATGCAGACTCCTGGATTTCACAAACAATTACGGATTCTCATATCCAATTCACCAAGTCATGGATGGAAGCTCACATAGAGGATGCTGAGAAATATCTGGGAATGCCGGTTGTGTTCGCTGAGTTCGGTGTATCTTCAAAAGATCCTGGATACAACTCATCATTCCGCGACACACTAATTAACACAGTGTACAAGACCCTCTTGAACTCAACCAAGAGAGGTGGGAGTGGAGCTGGGAGCCTTCTGTGGCAGCTTTTCCCTGACGGGACAGACTACATGGATGATGGATATGCAATTGTTCTATCAAAATCTCCTTCCACATCAAACATCATTTCCCTCCATTCAACACGAGTTGCAATCGTCAATTCCATGTGTTCATGGAAATGCAAATGGGGCTGCAAGAAGAGGAATCCTTTAGAGGCATTCCTCTACCATGATGATTTGTAAAAGTAGTTGATCGGTCACAACAGGAAAAGGAAGAAGTACAGTGGTTATGATAATACACCCTTGTTTTTGTACCTCCAAAAACATGAATACTGGTGGTATTAAATAAATAAAATGGCTTTGCAGGCTGTTATTGTACAAAAAAAAAAAAAAAAAA



gi|60694485|gb|DN485101.1|DN485101 M106B03.3pR Populus female catkins cDNA library Populus trichocarpa cDNA clone M106B03 3', mR

TTTGGATTGAAGGGAGCTCTA
||||||||||||..|.|||||
AAACCTAACTTCTTTTGAGAT

EST sequence (targeted fragment marked with yellow):
AAAAAAAAGACATTCTAGCAAATTGGCTTTATCAAGGCATTAAACATTACAACAATCAAGGTTTCGACAAATATAAGAAGGTGGGCCAAGCAATTTTTTTTTTTCTTTCAAAACTTCATTTTGATCAAGCTATCACTGTAATTGATGGGCAACCCAGACATAACTCTTTTCTAATCGTCGCTAGAGTTTTCTTCAATCCAAAGGCTAACCACCACACCCATGAACTGATTCTTAACCGTACAAATGGCTGGATTTAAGACCAAGGAGGAGAGAAAATTCAAATAACTAGCACAAAGCTTTGGGTTGTTTACTTCCCTTTGCCCATAAAATTTCTACTAGATCTGACCTTCAATTGAGATGAGAAGCCAATTTGGAGCTTTGTTAGGGTTTTCTTAAGAGGGAGAAATTATATCTAAAAAGAGAGAGAAGGGTCTGGAGTAGGATTCGTCGTGTTTGTTTTATAAAAAAAGAATGGGTAATTGAAGCCAAGCCAGACCTCCTCCTGCAACACACACCACCTGACATGGAAGAGGAGGTCACAACGAATTCAACAAAATAAAAAAGACATCAATCAGAGCTCGAATGAATGCTAATATTTTGCCCAAAGGATGTAGGCCTTCCTCATGCCCTGGCGTGTGCTTCACAAGTAGGGAAGAAAGGGCAAAGGTGTAAAAGGTTACAAACGCGGGACGTAGGATTTGTAGGGCCAGTGGTTGAGTTTGGTGGGGACACCTCATGAGATTTTTCCTGTGACAACGCGCTTGGGCCTTCATATACATTGGATTTGGGGGTTTCCTTTATTTGTTAAAACAATTATTTTGTGAATTTCGGC



gi|52497190|gb|CV244215.1|CV244215 WS0254.B21_A22 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS0254_A22 3', mRNA sequence

TTTGGATTGAAGGGAGCTCTA
.||.||||||||||||||.
CGACTTAACTTCCCTCGAGGA

EST sequence (targeted fragment marked with yellow):
GAATGCCTGCTAGCAGCTGGAGGAGGATATGCGCAAATCCCAACCTTCAACGTCTGTAATTACTGGCTGGCAGGAGCTCCCTTCAATTCAGCTGGCTTTGCTCAATTCCTTATGTTATCTCGCCATTATTCTGTGCTGTTCAAAGGTGTCATGAGCAGCTGGTGAATAGCAACCCCATAATCTGTATAGTCATTTCCCTTGGGATAGTAAGTATCCCTCTGACTTGTCGAGAAGTTAATAGAATAACTCGAAACTTTAAACAATGGCGCTGTAAAACTCTCTGCCGTGTAGAATGAGAGTTGCCATCGTTTTGACAAATGAATTTCCGTTGCGTACACAAAGTTATCAGATTTTTAAGCTGAAGTATCTCAGTAAAAGAAAATAGAAAGTGTCAAAGCTGAAGATTTGGTATCGTCACATATGTCTAGAGTTCATTTACACCTATCATCATGTCTGCTTGTGGCGCGCACCAACCGCCAAGTGATTCGGTATTGTGAATCTTCGATAGCACTGCAAATGTCCTAGCAGTTAGCACTGCAGAAAAATGTATCCATTGTTAATATTTCAACTAAACCCTAGCAGCCTACCGTTTGGCAAATAAATTCGAGGAATCCACAAAAAAAAAAAAAAAAAA