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mature miRNA: TTTTGGGACCGAGGGAGTATT
species: Triphysaria pusilla
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|159705563|gb|EY163590.1|EY163590 CBTN9466.g1_C16.ab1:P Triphysaria pusilla root tip normalized cDNA library Triphysaria pusil

TTTTGGGACCGAGGGAGTATT
||||||| ||| ||||.| ||
AAAACCCGGGCCCCCTTAAAA

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCAAAAAAAAAAATTTTTTAAAAAAAAAAAAAAAAATGGGGAAAAAGGGGCCCCCCCCCAGTTTAAAAAAACCCCAAACCCCAAAAAAAAACAAACCCTTTTTTGTTTTTCCCCCGGGGCCCCCAATTTAAAAGGAAAGGGGGGGCCCCCCGGGGCCCCGCCCAAAAAAAATGGGATTTTTTACCCCAACCCCCCCCCCTTGGTTAACCCCCCCAAAAAGGGGGGATTTAAAATCCCCCCCCCCTGGGAAAAAACCCTTAACAAAATTCCCCCCGGGGAAAATGGCCCCCCGGGGGGCCCCCCCCAAAGGGCAAAGGGGGGGGGGGGCCCCCCCCCCCCTCCCGGGGGGGCCCCCCCCTAAAATCCCAAAAAAAAAAAAATTTTTTGGTTGGGGGAAAAAATTCCAAAAAAAAGGGCCCCCCCCGGAAAAAAAAATTTTCCCCCCCCCAAAAATTTTGGGCCCCCCCCCGGGGGGGGGGATTCCCCCCCCCCAAAAATTCCCCCGGGCCCAAAAAAAAACCCTTCCCCAAAAACCCGGGCCCCCAAATTTCTTTGGGGCCCCAATTTAGGAAGGGGTAAAAAACCCCGGCAAAAAAAAAAACCCCCCCAGGAAACCAAAGCCCCCGGGTTGGGGGAAAAAATTTCCCCCAAAAACCCGGGGGGGGGGGA



gi|159601249|gb|EY135027.1|EY135027 CBTH4762.g1_D15.ab1:P Triphysaria pusilla root tip normalized cDNA library Triphysaria pusil

TTTTGGGACCGAGGGAGTATT
||||||||||| ||||.| ||
AAAACCCTGGC-CCCTTAAAA

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAAAAACAACCATTTGATTTTATTTAAAAATTCAAAAAGAAAAAAGTACAATCAATTTCCCAGGGTTACAAAGGGAATCAGGACCATTTTTGTTCCTTATTTACAACAAGTTAATTAAAAAAGCCCTAAACATTTTGAAGATTCTTCCGAAAACTAGGAGGATTTGAACAAAATTCCCCGGTCCCAAAAACAGCTGGAAAAATTTCCCCAAAATTTTCGGCTTCAATCCCTTTTTCCTTCTGGAAGACTTCTTTTTGAACATGCTTATCTTCTTCAACTGAAAAAACCACAGTCTTCGGTCCATGTTGGCCTTGCACAAACGCCTCTTTCACCCCCCAATGTTTGTCGGTTCAAAAGAAATCGGTCCCCTCTTCAACTTCAAAGGCAAACTTCTTTTTCCCCTTCCCCTTCTTGAGCAAGCCCCACAAAGCGAACAAGCAAAATACGGGGAAAAAAATAAAGCCGAATGAAACGAACCCCACTATGATTACGGTGGGCCCGTTATCGTCGAAAAATAAAAATAAAGGAGGTGGTGAAAAGGCCATTTTTGAAATTGTTGGGAATGTTGACCC



gi|159679315|gb|EY149778.1|EY149778 CBTN11917.g1_I03.ab1:P Triphysaria pusilla root tip normalized cDNA library Triphysaria pusi

TTTTGGGACCGAGGGAGTATT
||||| ||||| ||||. |||
AAAACACTGGCCCCCTTTTAA

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAACCAAATTTCCCCCAAAACCCCCAAAAGAGGGGGGGTTTCCCCGGGGCAAAAAAAAAAAAGGAAACCAAAACCCATTTCCCCCACAAAAACTTCCCCATTCCCCCCCCCCGGGCCTTTTAAAACCCGGGGTTTTTCGTAAAAAAATAAAGCAAACAAGGGGGGGGCCTTGGCCCGAAAAAAAACCCCCCCCCCCCTAGAAGGGGGGGTACCGCCCGTAAAAAAAATTTTCCCCCGGTCACAAAAATGGGAGCTTTTCCACCCGAGGGGGCCGCCCCTGGGAAACCCCTTTCTGGGGGGGGGAAAAAACCCGGGGGAGGCCCGTTCCGCATTGGGAACCATTTCCGGGGGGAAACCCCTTTCCCCCCCCCAAAAAAAAAGCCCCCAAAAAAGGGGGGTTGGCCCAAGGAAAAAAATTTCCACAAATTGCTTAAAAGGGGGTTTAAAAAAAACCCCCCAGGGGGGGGCCTCCGCGGAAAACCCGGGGGGGCTTTCCAGGGGATCCCCCCCAGGGCCCCCGTATTGGGGCGGCCCTTGTCCCGGATTAAAAAAATTGAAAAGGGGTCCGGGAGTTTTTCCGGGGGAAAGGAAAAAAAAATTGCTTTGGGGCCGGGGAGCAAAAAAAGGCCCCCCCTTCTTAAAAAAGGCCCAGCCCCCCCGGGGGAAAGT



gi|159742375|gb|EY133637.1|EY133637 CBTH4127.g1_M24.ab1:P Triphysaria pusilla root tip normalized cDNA library Triphysaria pusil

TTTTGGGACCGAGGGAGTATT
||||||| ||| ||||| |||
AAAACCCGGGCCCCCTCCTAA

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCCCCAAAAAAAACCTTGCCAACAAAAAAGGGTTGGTTCCCCATTCCAGAACCAAAAAATATGTACCGGGAAAACCACCAACCACCCTTTGTTTGACCAGGGCCTTGACCAAGATTCCCCAGGGCCCCATTTTCCATTTGAAAACCGATCAAATTTGCCACCAACAATCCTGCGTCTGAATCTGACCCGTCAAAATAATCCTCCCCCGGGCCCAAAAAACGATCCCCCAATTCCCCCCATCCCGGGATAAAACGAAACTCTTCATTCAACGACACATCGATCTCTGAAGTAACAATTGTCCGTGACGGGAAACCTTTGCAAACCCATTGTATTCCCCCCCGGGCCCAAATGAGGTTTAAGAAAAAAATGGGCGAATCCGGAACCCCCTTTTGTACAAATAACCCCATCCCCCGAATAACAAAATTGCCCGTAACAAAAACAGGGTCCAGGAGGAAAACCTGGCCCCCAAAAAAATCTTTGGGAAGTTTCCCCGAAAAAAACCGCTTCCCATTGGCCCCCTCCCGGGGGAACAAAAACCTTCCCATTTTGATTCCCTTGCAACAAGCACGGAGGGCATTTTCCCTACTTTCACCCCTTCCAAAAATGGAAACCCCCCCCCATTTCTTGCGAAAGTCCACACCCGT



gi|159693571|gb|EY146517.1|EY146517 CBTN10381.g1_I03.ab1:P Triphysaria pusilla root tip normalized cDNA library Triphysaria pusi

TTTTGGGACCGAGGGAGTATT
||||||.|||| ||.|.||||
AAAACCTTGGCCCCTTTATAA

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAACCCGGAAAAAAAAAACCAAAAAAAAAAAAAAGGCCCCCCCGGCCCAAAAGAACCCAAAAAAAACAGGGAAAAACCCCCCAAACCCCCCCCCCCAAAAAAAAAAAATTTTCCCAAAAATTTTTTTTTAAAAAAACCCCAAAAAAACAGGGGGGTCCCAAAAAAACCCCCCCCTTTTGGAAAAAAAAACCCCTTTTTAAACTGGAAAAAAAGGGCCCAAAAGGGGGCCCCCATCCCCCCCAAAAGCCCCAAAAGGGGCCCCCCGGCCCAAAAAAAATTTCCCGGGGGGGGGGGAAAGCCCCCTTGCCCCCCGGAAAAATTTCCCCCCGGGCCAGGGGGACTTAGGTTGGGCCGAAAGGGATCCCCCAAAAAGGGGGGAAAAACCCCCCCCCCCCCCAAAAATTGGGGGGGAAACCGAAAACCATGGGGGGGAAGGCCCCCAACCCCCCCCAATTCCCAAAACAAAAACCCCGGGAAGGGAAAACCCCCTGGGGGCCCCCCAAGGCGGGGGCCAAAGGGGGCCGGGAAAAAAACCAGGATCCCCGGCCCCCCCGGGCTAATGGGTTCCGGGAAAAAATGGAAAATCCAAACCCGGAAATCCGGAAACTTTGAACAAAAAACCCCAAACCCCGCAACAAATATTTCCCCGGTTCCAAAAAATTTACC