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mature miRNA: TCTCTCCCTCAAGGCTTCAGA
species: Artemisia annua
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|159640626|gb|EY038822.1|EY038822 CAIY4041.fwd CAIY Artemisia annua leaf Artemisia annua cDNA clone CAIY4041 5', mRNA sequence

TCTCTCCCTCAAGGCTTCAGA
||||||||||||.. .||||
AGAGAGGGAGTTTTATGGTCT

EST sequence (targeted fragment marked with yellow):
GTTCTAGATCGCGAGCGGCCTTATATACCACAACGGTGGCTGCTAGGATTCTTGCGAATTTGCTTGTATTGGGTGGTGGAATCTTGGCCAGGGGAATGGTTCAAGCTTATCGCCAAGCCTTGCAGAATGCCTCAAAAACAGGTGTTGCGCAAGAAACTATGCAAAATGCTGTTCGTAGAGGAAGCAAAGCTATGACTGAACTAGAGGCTAGACAAATTCTTGGTGTGACCGAAAAGGCATCATGGGAGGAAATTGCACAGAAATATGATAAATTATTCGAGCGCAACGCTACGAACGGGAGTTTCTATCTGCAGTCGAAGGTTCACCGGGCCAAGGAATGTTTGGAGACAGTTTATCAACCAAAAGATATGCCAGGTGGTCCAATGGGCTGACTTAAACTTTCTATTTTGACCTAAAGACTTTTGGAAAAGACTATTTGGCAACATATGTATATTATTAGTCTGGTATTTTGAGGGAGAGAAATTTTTGTATTCTTGAATCTGTTTCTGTAAGCCATTATTGGCTATCTTTGTGTCACATGTTGAATGGACTACTGTAATACGAGTACTAATAAGTTCACAATCTTAAGCCGTTATTGCTTATGTTCATTAAGAACATAAACCGATGTAAGGCTTCAGATGTTACCTGANAAAAAAAAAAAAAAAAAAAAG



gi|159687713|gb|EY093156.1|EY093156 CAZI21082.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI21

TCTCTCCCTCAAGGCTTCAGA
||||||||||||.||| | |
AGAGAGGGAGTTTCGACGACG

EST sequence (targeted fragment marked with yellow):
CGATGTTGAAGTCATGAATGCAGACTCAAGATTGGTTCTTGGTGACCAAGATTCCTTGCCTAGTCCCAGTAACGTTGCCCGAGCGCTCTCTTCTTTGTACAAAGAGATAACTGATACTGTCCGCAAAGAGTCAGCTACCATCATGGCTGTATTTCCTTCTCCAAATGATGTCATGTCAATTTTGGTACAGCGAGTAATGGAAGACCGAGTTCCTAAACTTCTAGAGAAGCTATTGGTGAAACCATCTTTAGTAAACCCACCTCCGATGGCAGAAGGTGGTCTGTTGCTATACCTCAGAATGTTAGCAGTGGGATATGACAAGACACAAGAACTTGCCAAAGATCTACGCAGCGTGGGTTGTGGTGACTTGGATATTGAAGGCCTAACAGAATCCCTATTCCTTGATCACAAAGATATGTACATCGAATATGAGCAAGCTTCTTTAAGACAACTCTACAAAGCTAAGATGGACGAACTGCTTTCTGAGAGCCAGCTATCTGGAGAATCAACAGGTTCGATTGGACGTTCAAAAGGAGCTTCAATATCATCTTCCCACCAGCAGATATCTGTTACAGTGGTCACAGAGTTTGTGCGTTGGAATGAAGAAGCAATTACAAGATGCACTTTGTTTTCACCTCAGCCTGCCACTCTTGCAGCCAACGTCAAAACTGTCTTCACTTGCCTTCTAGACCAAGTCAGTCAATATACAACAGAAGGGCTTGAAAGAGCTAGAGATGGTCTCACGGAAGCAGCAGCTTTGAGGGAGAGATTTGTGTTAGGCACTAGTGTTA



gi|159763237|gb|EY114711.1|EY114711 CAZI8999.fwd CAZI Artemisia annua normalized leaf library Artemisia annua cDNA clone CAZI899

TCTCTCCCTCAAGGCTTCAGA
||||||||||||.||| | |
AGAGAGGGAGTTTCGACGACG

EST sequence (targeted fragment marked with yellow):
GATCAATATACAACAGAAGGGCTTGAAAGAGCTAGAGATGGTCTCACGGAAGCAGCAGCTTTGAGGGAGAGATTTGTGTTAGGCACTAGTGTTAGTCGAAGAGTGGCTGCTGCTGCCGCTTCCGCAGCAGAAGCTGCTGCTGCAGCTGGTGAAAGCAGTTTTAGATCTTTCATGGTTGCTGTACAACGTTGTGGAAGCAGTGTTGCTATTGTTCAGCAATACTTTGCCAACTCCATTTCAAGGCTTTTGTTACCAGTGGATGGTGCACATGCTGCTTCATGTGAAGAAATGGCAACAGCCATGTCCAGTGCAGAAGGTGCTGCCTATAAAGGACTTCAACAATGCATTGAGACAGTTATGGCCGAGGTGGAACGATTGCTTTCAGCTGAGCAAAAAGCTACCGACTATAAGTCACCAGATGATGGCATTGTTCCTGATCATCGCCCTACAAACGCATGCATAAGAGTTGTGGCATATCTCTCCCGTGTGCTTGAGGCAGCATTCACCGCTCTTGAAGGTCTTAACAAGCAAGCTTTTCTAACCGAATTGGGGAACCGCTTGCACAAAGGCCTAAGCAATCACTGGCAGAAGTACACTTTTAATCCAAGTGGAGGATTAAGGTTGAAACGTGACATCACTGAATATGGGGATTTTGTGCGAAGCTTCAATGCTCCTACAGTTGACGAGAAATTTGAATCATTAAGCATCATGGCCAATGTATTTATTGTTGCCCCTGANAGCTTGTCGAGTCTTTTTGA



gi|159599792|gb|EY038582.1|EY038582 CAIY3923.fwd CAIY Artemisia annua leaf Artemisia annua cDNA clone CAIY3923 5', mRNA sequence

TCTCTCCCTCAAGGCTTCAGA
|||| ||||||| ||..||.|
AGAGCGGGAGTTACGGGGTTT

EST sequence (targeted fragment marked with yellow):
GGACAATCCATCGATTTCTTTGGGGCATTGAGGGCGAGAGTGTATGATGATGAAGTGAGGAAGTGGATTGGTGAAACTGGGGTTGAGGGCATTGGGAAGAGACTTGTGAACTCAAGGGATGGCCCACCCACATTCGAACAACCCAAGATTACCATCGATAAGCTCTTGGAGTACGGAAACATGCTGGTTCAAGAACAAGACAATGTCAAGAGAGTCCAGTTGGCCGAGACCTATTTGGACTCAGCCGCACTTGGGGATGCTAACAGAGACTCCATTAGCCGAGGAGAGTTCTATGGGAAAGCAGCCCAACAGGTTCAGGTTCCTATTCCAGAAGGGTGCACTGACCCTGGTGCGGAGAACTTTGACCCAACAGCTAGGAGTGATGATGGAAGCTGCACTTACACTTTCTAAATTTAGAAACTTTAAGGGAATTACAAGAAGTATTTGTCTAAGGATGTGAGGATGTTAAACAGTTCTATTTATATGGATTGTTGCGACCTTTGTGATTTTGTTTGTTGATTATTATCAAATAAGTTATTTTTGGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA