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mature miRNA: TGACAGAAGAGAGTGAGCACA
species: Avena barbata
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|240330404|gb|GR340112.1|GR340112 CCOW2020.b1 CCOW Avena barbata leaf, pooled from different levels of rain and nitrogen (L) A 

TGACAGAAGAGAGTGAGCACA
.|||| |||||| |||||| |
GCTGTGTTCTCTGACTCGTAT

EST sequence (targeted fragment marked with yellow):
GGGGGACCTGGTGGTGTTCCCCAAGGGCATGAGCTGCACCTGGGACGTCGCCGAGGCCGTCGACAAGCACTACAAGTTCGAGTAGCCTACCACAGCCGCTACGCTACTTTCTGGTCACTACTAGTACTAGATCGAGATATTCCTGCGCTCGCGTACCTCGTACAGCTCGATTATGCTCAGTCTCTTGTGTCGGAATAAAACGCAGATTAGTGGATTGTGTTTGCTGAATCTTCTGTGAGGTGCTCGTGTGATTAGAACAGTGAGCATCTTCGTCTT


gi|240307765|gb|GR327971.1|GR327971 CCOS815.g1 CCOS Avena barbata leaf, grown under high rainfall (H) Avena barbata cDNA clone C 

TGACAGAAGAGAGTGAGCACA
|||||||||||||||||||||
ACTGTCTTCTCTCACTCGTGT

EST sequence (targeted fragment marked with yellow):
AGGAGATGCATGTATAAATCACCACAGCGTCGCAGGGTACCGGCAGCATCTCCATGATCCAGCTCCCAAGGCGAGTACACTACAAAAGGAGAAGAAAAAAACATTTTGGGGATGAGAAAAACAAGTAACAAATCCAATCATTTATTCCAGTACCGCAAGCGCCTACAGCTGCTGCTCCTTACAATTACCACTGGCATCCGTATCACTCTCCTCGGTAGTAGCTCCCAAAGGCAACTTGACAGGAAAGATCAAAAGAACAGAGCGAATTTTTTTTTTGCTTCCCCATACATCTAAGAGATAAGGAAGTGGCATGGTAAAAAGTAGGAGAAAAGCCTGAGTACTTGCCGCATGCGTGTTGAGTCTTTTGTGGCTGAGTTTCTTTCTTACAAGCAGGGTTTAACCTTCTTTCTTGTTGGGAAAAATATGAAGTACCGAACTGAAATTGAATCGAATTGGCTCTACAAACCCACTTGTGGATCTGAATGTAAGGTGAACTCCCAAGAGAAAGAGGCTGACAGAAAGAGGAGTGAGCACGCGGCGAAGACGGCATACATGTTATGCCGGACATCACGCCGTGTGCTCACTCTCTTCTGTCAATCTCTTGAGGGATTCTCCGCGGAGGAGCTGACCTTACTTTGCAGATTGCACAATCGAAGACCAACTTCCAAAAATCGGCACACAAATTGCCAACAACCCAAAAATCGGAAAGAAAAACCCAAATAAAACCTCAGAACACAGCAGGAAGCCGGAGAGGTTTTTTCTTCTTGGGATCCGGTGA


gi|240334595|gb|GR342052.1|GR342052 CCOW3172.g1 CCOW Avena barbata leaf, pooled from different levels of rain and nitrogen (L) A 

TGACAGAAGAGAGTGAGCACA
||||||||||||| |||||
ACTGTCTTCTCTCTCTCGTAG

EST sequence (targeted fragment marked with yellow):
GCTCAAATTTACAGAAACAAAACCTTCTTGGTTAAAACCAACAACTGCTGCAAGTGCTGATGGGACACATTTATCTAGTCAGCACGTGTGGCACAATATTATGCCGCATGGTGCACATCATGGTTTTGATGGGTTCGTGGCATTCAAGGGAACTGGTGCAAAGTTCCTTAATCAAGGCGTCGAAGCTTCTGCTGTCGCTTCCGACTCCAGCAGAGCCCCAGATCTTCAGGATGCTCTCTCTCTTCTGTCAAGCAACTCAGTGGGTGCTGCCAACCTCCAGCAAAGTCCACAGATGCACCCCGGGGTGGCTGCCATTACCGGCACCTCCAACCCTGCTATGCACACGCTGGGCTCATCGCCAGGGCTCTGGCTAGATGGCCCACCACTTGATGATCACCCGCGCTTCCAGGTTTTCGACCGCTCGGGGGGCCATGACAGTGAGCTCCAGCTCCCAAAATCTTCCTACGAACACGCCTCCCACTTCAACCGGATGCACTGATGATGATGCTCATCCGTTCACTGCCCTCGGCGCCCGATTCGATCATCACTGTTTCTTCAGTCTGAAACTCTGAATTCTGAATTTCGTTGTTCGGTGATGTTGCCTGCCTAGAGGTTTACGTTTTGGTGATGCCAAGAATAAAGGCGCACACAGAAAAACTATGGATCGGATTGATCTGGTGGTGTTGCCAGCCATGGAAAAAGAACCTGAGCTTGCCAGGAGCTACCCATCATCAACCTTTTGTCTGAATAAATTCCTAATTTGATATT