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mature miRNA: TCTGTCGATTTCTCAATCGAA
species: Barnadesia spinosa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|211692788|gb|GE549788.1|GE549788 CCHS8592.b1_P11.ab1 CCHS Espina Barnadesia spinosa cDNA clone CCHS8592, mRNA sequence

TCTGTCGATTTCTCAATCGAA
|| |||||||| .|||.|.||
AGTCAGCTAAAAGGTTGGTTT

EST sequence (targeted fragment marked with yellow):
AATCAACACACTCCATCTCTCTCTTTCATCCGATTAAATCACTTCAATTAACAGCAACAGTTTCTGCTTTTCTCATTGTTGCTTCTCTGATGGAGATGGATACTTCAAACCCTGCTGTCTTCGTGAATGCTGACCTGCTACGCAGTTACGTTGGCCGGAGAGTACGCACTGTTATCCAGGTATTGCGTCCTGATGGTGGTGGTTTGGTTGGAAAATCGACTGATGAACAACAAATTGTTGTAAAGGGCCAGCCACCAGCTCCTCTTACTACATTTGTCGAGGTAATCGGTATTGCTGACAGCCCCCAATCCATCCGTGCAGAGATCATGACCAACTTCGGTGATACATTTGATACAAATAACTTCAACCAACTTTGTCAACTTGCGAACGGGGAGTTTAGGCATTTGTTTATCTGAAAGAGGCTCTATGATGAGAATATGCTACGAGTGACTTGAGGAAGTGTGGACTGCCTACACTGATAGTTCCAGTAGTGACAACGCATGTGAACTCAAACAGTACTGTTACCTTTTTCTGTTTATTCCGCGTTTGACGTTTCGAAATTGTAGGGTAGATATAAGTTGTCTGGTCATTCAGCTTGTAGTAATGCAAAGTGTAGTAGACCTCTTCACTATCTGAAATTCAATTCTATGGTTATCTT



gi|211662539|gb|GE528598.1|GE528598 CCHS15483.b1_F07.ab1 CCHS Espina Barnadesia spinosa cDNA clone CCHS15483, mRNA sequence

TCTGTCGATTTCTCAATCGAA
|| |||||||| .|||.|.||
AGTCAGCTAAAAGGTTGGTTT

EST sequence (targeted fragment marked with yellow):
AATCAAATCAATCAACACACTCCATCTCTCTCTTTCATCCGATTAAATCACTTCAATTAACAGCAACAGGAGATGGATACTTCAAACCCTGCTGTCTTCGTGAATGCTGACCTGCTACGCAGTTACGTTGGCCGGAGAGTACGCACTGTTATCCAGGTATTACGTCCTGATGGTGGTGGTTTGGTTGGAAAATCGACTGATGAACAACAAATTGTTGTAAAGGGCCAGCCACCAGCTCCTCTTACTACATTTGTCGAGGTAATCGGTATTGCTGACAGCCCCCAATCCATCCGTGCAGAGATCATGACCAACTTCGGTGATACATTTGATACAAATAACTTCAACCAACTTTATCAACTTGCGAACGGGGAGTTTAGGCATTTGTTTATCTGAAAGAGGCTCTATGATGAGAATATGCTACGAGTGACTTGAGGAAGTGTGGACTGCCTACACTGATAGTTCCAGTAGTGACAACGCATGTGAACTCAAACAGTACTGTTACCTTTTTCTGTTTATTCCGCGTTTGACGTTTCGAAATTGTAGGGTAGATATAAGTTGTCTGGTCATTCAGCTTGTAGTAATGCAAAGTGTAGTAGACCTCTTCACTATTTGAAATTCAATTCTATGGTTATCTTATGAAAGCTTTTCCCTTTGTTTAA



gi|211674942|gb|GE536059.1|GE536059 CCHS22598.b1_L10.ab1 CCHS Espina Barnadesia spinosa cDNA clone CCHS22598, mRNA sequence

TCTGTCGATTTCTCAATCGAA
|||||||||||||||| .||
TGACAGCTAAAGAGTTA-TTT

EST sequence (targeted fragment marked with yellow):
AATATTCAGGTCGAAAAGTTCTTTGGGGCAACGTAGTTGCTCAGAAGTTGGATAGCTTGATGGTGCTGACTTTCGAACACCTTAAATCCTGTTACGAAAGCGGCCGCTTGGCCCAGTTTGTGATGTTCTATGCTTGTTCCCTTGACCCCGAAAACTGTGGGACACAATTTGTGAGTAGACTTATTGATATCTTTGAGAGCACCGCCTATCCCAAAGATTGGAGAATAAGTGCCTTGGCTTATCTTGCGAGTTACCTGTCTCGAGCTAAGTTTTTGTCAGCCTCGTACGTGACTACGATATTGGAAAGTTTGGTGGATTGGTGTCTTAAATATTGCGAAAGCCGGGGTGGTGAGATTGATCCCAAAAATCATCGAGTTTTTTATGCCGGATGCCAGGCCATTATGTATGTGCTTTGCTTTCGTATGAGATCGATTCTGGGCACTCCTCGACTCAAATCACAACTTTTCATCATGCCCATAGAGACAATTTTGAAGCATCCGCTGAATCCGTTAGAGGTGTGCTTACCTTCCATAGTCGAGGAATTCCTCCGTCAAGCCCAAGCCGCTCGTCTCTTTCACGTGTCGGGAGCCTTTGTATTCACAATCTCCTTGAATCGGATTTGTCTCGAGCTTTTGGTGGATCGGAAGGCTGGATTTGTTCTCCCGTTCGATCATATTTATTGAGAAATCGACAGTTTATCCGACCAACTCATATCTGGTGATGGGAGACACTTACACATATGTGCCGTGACATGATGAGAAAGAAG