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mature miRNA: TGCCTGGCTCCCTGTATGCCA
species: Brassica oleracea
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|308683627|gb|DK540095.1|DK540095 DK540095 full-length kale cDNA library (flowers) Brassica oleracea var. acephala cDNA clone

TGCCTGGCTCCCTGTATGCCA
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ACGGACCGAGGGACATACGGT

EST sequence (targeted fragment marked with yellow):
TGTGTAGACAGTTGTGCGATTGATAACACAATTTTTAATATAAGGATTTACAAGAACAAAAAAAGAACAAATTATAAGGGGAGCTCACAAGTTATATACATATACAAACAAATTTATAAGATCGATATGATTGAAACGATACAAAAGAATCTGAACCGTACGGGAATGTAATAATCAGCTATCGAAATAATTAACCATTTCCTATAGAAAAAAACAAGGAGCTACGTCGATGTGAACGCAGGAGTCCTCGTAAGAAGTGCTGTGACGACATGACATAAAATTGTACTGGAAAAATACGAAACGAAAGACAAGAACGGAATCAGAGCTCAACAAAATATCTATCAGTAAAATCTTTTGGAGACCACAGGAAGAAGCATATGTATATGGTTTAGTGAAGTACCTTCTAATCTTCCACACATGATGGATAACAAGCTCAACACAATTTAACGGGTACCATAACATAATTAGAATGGTGTAGGAACTTTAACTGGTACCATAAAATAGCTAGAAAACGTATGTAAATGTATATGAAAATATGCATGGCTCCTCATACGCCATTCACGGAGGTCATCAATGGTCGATGAGCTTAGAGGATGGCATACAGGGAGCCAGGCATACACATCTTATACAACTACACATATGCATCTATGTATGTGGCCTGATGCATATAAATGCTATTATGTATTGAAACTAATTTCCCAAACCTGGATCTATTTACGGTAAGTTATCTAGATCTACACCTGGACATGCATAAACAATAAGTAATAAATATGAAGGAATAGAAGCAATCTAAAGCATAGATCGAGA