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mature miRNA: GTTGCTTTGAAGAAGAGAGGG
species: Centaurea maculosa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124618660|gb|EH716371.1|EH716371 CNML2703.b1_M03.ab1 CNM(LMS) spotted knapweed Centaurea maculosa cDNA clone CNML2703, mRNA s 

GTTGCTTTGAAGAAGAGAGGG
|.||||||||||||||. ..|
CGACGAAACTTCTTCTTCTTC

EST sequence (targeted fragment marked with yellow):
CTTCGGCGGGGCCAAACAGAGATAACAAAACAACACCAAATCAAAAGCTAGGGTTTCTTCTCTTCTTTCTTCATCTCTCTCTGTAGATCTTCTTCTTCTTCTTCAAAGCAGCAGCCAGCTACCAGCAGCTATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGCAAAAGAGGTAGGAACGAAGCCGACGACGAGAAGCGTGAGCTCGTCTTCAAGGAAGACGGCCAGGAGTACGCTCAGGTCCTCCGTATGCTCGGCAATGGCCGCTGTGAAGCCATGTGCATCGACGGCACCAAGCGTCTCAGCCACATCCGTGGTAAGATGCACAAGAAGGTCTGGATCGCCGCCGGTGACATCATCCTGGTCGGTCTCCGTGACTACCAGGACGACAAGGCTGACGTCATCCTCAAGTACATGCCGGATGAGGCTAGGCTTCTCAAGGCCTACGGTGAGTTGCCGGAGAATATTAGGCTTAATGAAGGTATCGCCGGTGGACTCGATGAGGAGGATGATAACGCCGGTGATGATTATATTGAGTTTGAAGACGAAGATATCGACAAAATCTAGGTTTCTGGATTGGATTATCAACAAAAGGACATTAATCGAGTTCGAAGAACAGGTAAAGGTTAGGGTTTGTACAACTTGAGCTCTTTGAATGTGATTTGCTTTTTAATATTACGAAGTATAACGGTCTTTCATGTGTAAGCTTTTGAACTATGGGGATTTATGCATTGATGGTTTACGCCAAAAAAAAAAAAAAAAGGC


gi|148298336|gb|EL935336.1|EL935336 Centeaurea_plate-013_D19.trimmed.seq Centaurea normalized cDNA library Centaurea maculosa cD 

GTTGCTTTGAAG-AAGAGAGGG
|| ||||||||| ||||.||||
CACCGAAACTTCATTCTTTCCC

EST sequence (targeted fragment marked with yellow):
CGGATTACCCGGGATGTAGGCTATTCAGCTATTGTACCTGTATTTGTTTGTGGTTTTATTTTTTTTGGAAATACAAGAGCTCTTTAACGAGCTTGATTTTGCTTTTAGACGAGCTCGAGCTTTGTTATTTAACGAGCCGAGCTTGAGCTCGTTTAGGCTTGTCGAACACTCGAGTAATAACGAGCCGATCCCGAGCTCTTATACATGCAGAACGAGCCGAGCTCGAGCTAAAAAATACATGTTCGAGCGAGCTCGAGCTCGAGCTCGAGCTTCTATAACCTTAAGAGAGCCTGGCTCGATAAGTATGAAGCTCGACTCGGCTCGGCTCGATAGTAACTTACTAATACATGGGGAACATAATAACAATTCTAATCCTTTTTATTAACACACACCTGATTATAAACTTATAAGGGCATTATAGGCGTTTTTCAATTATGTATCCCATTAGAATCCAATTGAAGACCTGCATCCCTTTCTTACTTCAAAGCCACCTCTCTTTCCTCTCCTCCACCCCCTTCCCGCCGGAGCTCCACTGCCCTCTTCCGCCGGAGCTCCACCGCCCCTTCCCGCTGGAGCTCCACAACCCCTTCCGTCGTCGTCGGCGGCGCCTACTCCCCTCCGGCGACTGTCGGCGTTACTTCTCCTTCGAATCTCTTTCTGACTGTCGGCAAGAATCCATCATTCCGGAAATTAAATCCCGGCGACCACCACCACCGCGAACCTCACCATCTTCCAGATCTATCATCACCGCCACCACGTCTACCACCGCCGCTGCAAATTCCGACGACCTCACATCGTCACGACTTCTAGATCTGTCACCACTCACACGTCAGATCTGCAAATTCAGGGACTTACCGCGGTCACGACTCACACGCACACACGTCTTCACCACCCACCGCGTCTGTTCACAAACGCGCCGGGGCGACGT


gi|124652079|gb|EH747003.1|EH747003 CNMS6452.b1_H06.ab1 CNM(LMS) spotted knapweed Centaurea maculosa cDNA clone CNMS6452, mRNA s 

GTTGCTTTGAAGAAGAGAGGG
.||| |||||||||.||||||
TAACTAAACTTCTTTTCTCCC

EST sequence (targeted fragment marked with yellow):
GCACATTTACACACTTGAAAGCTTAACGTCTGTCTCTTCTTCCCTATTTCATTTCACTCCCTCCCCCTGGTGGTGTCTAGTAGTCTAGGGTTTTTCATTTTCCCCTCTTTTCTTCAAATCAATCCGCCTAAAAGCAAGCAAGCAAGGCGGAGAGCTCGAAGAAAATGGCAAATCAAGGTGCCAAGAAGCGAAGGGACGAGAACACTCGTCATATCAACAATCTCTTTCGCATCATCCTCGCCTGCAATGCCATTTATGTTATCTTAAGGGCAGGGTTCTTCTATTCCAGTTTTACATGGCTACATTGGATTGGCCTTTTACTGACTTCCGGTGCCTACTATTTATGCTACCAACAACTGGCATCAATGGCAAAGCCTACCTATACTGATAACGGTGACCTTTTAGATGGTGGATTTGATATGAGCACCGGTGGTGCTTGTGGATACCTGCATGATGTTATCTATATCACTTGCTTTGTGCAACTCACGTCTATCATCTCTGGGAAGTTTTGGTGGATATATGCTGTGATACCGGGATTTGCAGTGTATAAAGGTTGGGGTTTGATTAAGGGGTTTCTCCCTCAAGGTTCACAGGAAGTTGAAGAGGATGAAAAGACTCGAAAGAAAAGAGAGAAGATGGAGAAGAAAGCCTCGAGAGGCAAATTTGTGAAAACAAAAACCCGATAGTTCAAATCGTTAACGTTTTCTCTTGTGATTTGAATATTTAAGCTGCGTTGTAATGATATATATACCCATACGTTAATCTGTTCGTACTAGATTTATGGACTAGTAG


gi|124643012|gb|EH738567.1|EH738567 CNMS1201.b1_A13.ab1 CNM(LMS) spotted knapweed Centaurea maculosa cDNA clone CNMS1201, mRNA s 

GTTGCTTTGAAGAAGAGAGGG
||||||||||||  ||||||.
CAACGAAACTTCG-CTCTCCT

EST sequence (targeted fragment marked with yellow):
CGCGGGGGCGCTTTCCTCTCGCTTCAAAGCAACGTTTTCTTTCTCCAAGGCTGACGATTAATCGCCCTAGGTGATGATTCGTATGAAGAAATCATGTATTTATCAAACCATATTCCTTCATCTGCAATTGATTTCTCACCATATTCAGGTGGGTTTTTTTTCTGTCGGAGCAGTGGAGAACATTATGTTAGATTGCTACTCTCGGCACTCTCAAATCACTACTCTCGGTCCTCTCAAATCATCACTCTCACCTTAGACTTGCAGAAGAATTCATAATCATTGTGGAACAGTGCTCAGACCATTGCGACCATTCAAGATGTTGCCGATCTGATCGAGAAAGCTAGCCGATCCGATCTAATCATTCATAAACCTTTAGAGAAGCTTTTACTTCCGTCAACAACCTTCCCTATATAACGGTAGGAAAAGGTTTTAGGGAGGGTTTTAAGTGGGTTTTAGGGAAGAAAATCTTACATTTAGTTACACTTTTTTCAAATCCTTCTCTAAAAATCACTTTTAGATACGCTTCCAAATCCTTCTCTAAAAACTTCCTTCTCTAAAACCCCATTTTCTTGTATTGAATATAAAGAACTTTTGTAGATTTTTCTTGCAAGAAAATAAGAAAGCTCAACTGTATATATNANAAAAAAAAAAG