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mature miRNA: TCGATAAACCTCTGCATCCAG
species: Centaurea maculosa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124631295|gb|EH727764.1|EH727764 CNMM14206.b1_L24.ab1 CNM(LMS) spotted knapweed Centaurea maculosa cDNA clone CNMM14206, mRNA 

TCGATAAACCTCTGCATCCAG
||| ||||||||||||| | |
AGCAATTTGGAGACGTATGCC

EST sequence (targeted fragment marked with yellow):
GGGGAGTGCCGAGCGTTGATTTAATCGCTTATTTACCATCTTTTTTGTACGGTGCCATACTCGGTGCTACATCGAACCGAGCGCCAGATAACCTTTATCAACGTGGGTTGCAAAAAAATCGTTTTCTACCGTTCACTTTCCACATTGAAGGAAAGATGCTTAATACATGAAATTGGTTCGTCTGTAAACTATCGGAGAAGGACTTCGGCGGAAGAAGGCATCTACTTCATACCAAATGGTACATCCGATTTCCGTATGCAGAGGTTTAACGAGTTGACCGGCGAAAACACAGCTCAACCTCTAGAAGCGGAGCTCTATATGGGAAGGACGCTGCAGGTACCTTTGGGCAGAAATGGATGTGCATATTTGCCGTTCGAGGAACTATGCAACAAGCCACTTGGTGCTGCCGACTACTTTGGATTATGTCGAAATTTTCACACCGTGGCCTTAGATGGTGTTCCGATATGTGGGCTTCACAACCGGACGGCAACATATCCGTTTGCCACTTTGGTTGATGTGATGTATGAGAACAAAGCACGGTTGATGTGTACGGCCGAGGGTACACCTTTTGATCTCTTCGAAAGGATTGCGACGGTTGCGGATGCTCATATTATGGCACCTAGAACCTCTTCGAGATCGAAGAATACTGACGATTTCTACCTTTGTGTTGACAATGAACTGCGCTTTGC


gi|124627241|gb|EH723979.1|EH723979 CNMM10396.b1_G07.ab1 CNM(LMS) spotted knapweed Centaurea maculosa cDNA clone CNMM10396, mRNA 

TCGATAAACCTCTGCATCCAG
|||| |||||||..|||| |.
AGCTTTTTGGAGGTGTAGTTT

EST sequence (targeted fragment marked with yellow):
TCTTTCAAATATGCCATGAACCTAGTATCATGAGGTACATCAAGATTGCTGCCACATTACGTGATAAAACTGTTCGTGATGTTGCCCTCAGGTGTAGGTGGATGGCGAGAAAACGAAGGAAACAGGATGAACCGAATATGGGCAAAAAGTTGAAAGATAAGAAGGATAATTTGGCTGAATCATCCTCAAAGCCAAGTATATCATCACTTCCGGCATTCGATGTGGCTCCCTTTTCTGTTTCCATGAATAGTCGGGTGCAGATTGACGGTATCACTTTTGAAGCATTAAGTGGCTCAATAAGGCAGTTACTGGAACAAAACAACCAAGTCCTTGGTCAGATCTCGGCTAATATTTCTTCAATGAAACTGCAGGACAACATTGACCTCTTCAGCCACATGAAGAATAACTTAACCGCCATTTTAAACGACATGAGGTATATGCCAGGCCCGCCATTACCCGTATCACTAAACGAAAATCTTGCTCATAGTATTTTACCTGCTACAAGTCAGACGATGATGTTCGAGGCATCAAGTGGGACGTATATGAAGCAAGAGCCAGGGTTTTGATGTGGAGGTTTTTCGACCCTTTTCTCGTTTGGTTGCAAGTACAGATTTGAAGCTGCTGTAAATCTAATAGGTACCACTCATGTGCAAATCCGTTAGGATCCAATTCCGATGATCAAGAAAATTTGTAGAAATCCCATTTTTGGAAAGCTAATAGGTCACCCCTCACCCTCGGTTTTCAGACTAGTACCCCCAACGGTTGTGGTAG


gi|124618331|gb|EH716065.1|EH716065 CNML2392.b1_O21.ab1 CNM(LMS) spotted knapweed Centaurea maculosa cDNA clone CNML2392, mRNA s 

TCGATAAACCTCTGCATCCAG
||||| ||||||..| ||||.
AGCTAGTTGGAGGTGAAGGTT

EST sequence (targeted fragment marked with yellow):
ACACGCACTATATAGTCTCTGTGTGTGAGAGAGAGAGAGAAACAGATTCTGTGTGTCTGAATCGACACAGCTCCTTCAGTCCTCCTCCTGCTGTCCAAGCTCAACCCCCCCTCCTTTGACTTTCAACATATCCAATTTTGACTTCCCCCCCAATCGCTTATCGAACTTCCACTTTTTAATCTCCAGATCAATTTAAACGATCATCTGGAAATATCATATCAATATGTATAGACCCTCGCCCATTAGAAACCAGAGGTCGAAAGGGATCAAGCTCAAACACGTTCTTCAAGTTTGTTTGCTGGTTGCGGTATGCTTCTGGTTGATCTTTCAAGTGAAGAGGTCCCACGACAAGAAACAAGAGTTTGATGCTAGCGATCCCAAGATCTCATCGAGTATACATTCACAGAGTAGCGATGAAATCATTAAGTTCGGAAGAAAAGACCTCCATCCGAAAATAGAGGAAACGGCCAAAGAAGTCGAGATACATGAAGAAGTAGAGGAACAAGAACAAGAACAAGAAGAGCAAGAACATGACGATNACAAACCCGAGGTCGAAGATCAAGATGATGACNAGATCGAGGAGGAGAAAGATGACGAAAGAGGGGGTGCGGATGATGAGATTGATGAAAACGAGCAAGAGGAGTTGGAAGTGGAGGTTGATCGAGAAGACATGAATACAACGGATGAAGGCAAGGAGACGGAAGAGAGCGAAGAGAAGGAAACCGA