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mature miRNA: TCGATAAACCTCTGCATCCA
species: Centaurea solstitialis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124659893|gb|EH754638.1|EH754638 CNSL13803.b1_F20.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL13803

TCGATAAACCTCTGCATCCA
.|.|.|||||||||||.| |
GGTTGTTTGGAGACGTGGTT

EST sequence (targeted fragment marked with yellow):
GTTTGACAAATTCACACTCACTCACTCTTTACTCTACCAAATCAAACCTCCTCCAATCTCTCGCAATTCCATTTCATTTTCGATCTTCTTGAAGAAGCTAGATTCAGATTTTCAAGCCCAATCTCTATCTACAATAACAACTACCAATCTATCTCTATGTGTAGCCAGATGATGAGATTTGGAGAAACCCTAGATCAATGCACAGATTTGTTCTGTTATTGAAAACAACCTCTTCTTAATTAACAACAATGGCGAAGCAGTCGACTTTATTCTGCTTCCACAAGAAGCGGTGGCTGTTACTACTGTTAGCCATGCTCTCCATCTCCACCGCCGTTGCCTTCATCATTAGGGCGGCGCGTGATTCTTCATGCGATCCTCAACAACAACATTTCGATGCAGGTAATCGGCTCCAATCGACAACGACGAAGGAGATCGGAGGAGTAACCAGCAGCAGCAGCCCCCTCAGTTTCATGAAGTCCAAACGTCTTCTTCTAGTCTCCCATGAACTCTCTCTTTCTGGTGGTCCATTGCTCTTGATGGAACTAGCATTTTTGTTACGTGGTGTTGGTGCAGAGGTTTGTTGGATAACAAACCAAAAACCATCTGGAACAGATGAGGTGATATACAATTTGGAGAACAAGATGTTGGACCGTGGAGTGCAGGTTT