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mature miRNA: TTGGATTGAAGGGAGCTCTA
species: Centaurea solstitialis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124679856|gb|EH774596.1|EH774596 CNSM6934.b1_K05.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSM6934, 

TTGGATTGAAGGGAGCTCTA
|||.||||||| ||.|.|.|
AACTTAACTTCGCTTGGGGT

EST sequence (targeted fragment marked with yellow):
GGCGGGGGGGATAGGAGGGTATTATTATGACTAGTGGAGCTGGAGGTACCTTTTACTGGGTTCGAAATCAGGAGGAATCTTCATCCAAATCCCAAGGAATCGTTGTAATTTTCGCTTGGGGTTCGCTTCAATTCAACGATTATGTCAATCTTTATTCGTCTCTTGGTTGGAACTCTCTTCTTGTTCTTTCTGATTTTCTCAACCCATTTTTTCCTGAGAGAGCCACATCACTTGCATTTTCGGTCCTCAATGAGCTTTTAGAGGAGTTAAGGGCTAGACCATCCCCTGTAGTCCTTGCTTCACTTTCTGGCGGTTCACAGGCTTGTATGTATAAGGTTTTTCAGATCATTGAAGGAACATGTGAAGCACATCTCAATCTGGATGATAGTCGATTGATCATGACCTGCATCTCTGGTCAAATGTACGACTCCGGTCCAGTAAATGTTACTGGCGATTTGGGTGCTCGGTTTGCTCTACACAATTCCGTTCTCAACCTGCCCGGTTCATCCAAGTTGGTATCTTTGGTTGCAAAAGGTGTCACATCAGGTTTGGATGCTCTATTCCTTACTAGATTTGCATCCCAGCGCTCCGAGTATTGGCAAACACTTTACTCCTCTGTCGGTTTGGGGGCCCCGTTTCTCATATTTTGTTCGGAAAATGATGATCTTGCTCCATATCCGGTTATATGCTCATTTGCTCAACGAGTACAAGGATTAGGAGGACATGTTCGACTTATTGGATGGAAGGACTCTCCACATGTAGGTCACTACAAGCACAATCCCATCC


gi|124664395|gb|EH759137.1|EH759137 CNSL510.b2_L07.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSL510, mR 

TTGGATTGAAGGGAGCTCTA
||.|||||||||.|| ||.
AATCTAACTTCCTTCAAGGC

EST sequence (targeted fragment marked with yellow):
GGATTGGGACGAAGCGTAGCAGGGTGAACTTTTTGAAGTTTTACATGGTTGGATCATCCATAGGAATACTTGTCTCAATTGCTTCTGTCATCCAAAGCAATCTTCAACTAAAGGTTGTTCATGACCCGGGTAACTGGGAAGCAGAGAATTTTTCTCTATTGAAGATTGCTTTTGTTTCTGCTGGACTGTTTGTACAAATGTTTTCTGTCATTACAACATCATCTCTCGTCGGAAATATGTCGCCTCCAAAAAGAGCTTCTTGACTTATTAAAATCGTCAGTAGTACTTGTCATCTCTCTCTCTCTCTCTCTCTCTCTCTATCTCTCTCTCCCACTAGAGATACATTTTTCATATTGCCTTATTTTTGTTCTTTATTTCTCCTGTATTGTAATACCCATGAAAACTTCTTAGAATCATGAAAAATGATCGGTACAATTTTTGGTATATCTCTGAGTACGTAGCTGTTGTGAGGTATGGTACGGATTTGATCTTTTGATTGCCTGTCAAGCTGTCACACTCGGGTGCTTTTTCGGAACTTCCTTCAATCTAATTATATTCTTGTGC


gi|124689501|gb|EH784237.1|EH784237 CNSS23472.b1_O11.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSS23472 

TTGGATTGAAGGGAGCTCTA
||||||||||||.|| |  |
AACCTAACTTCCTTCCACTT

EST sequence (targeted fragment marked with yellow):
ACACCAGATAGATATGGCGCTCAGTTCACTTTCATCACTACCAATCAAATCGTTAAAATTTTCACCTTCCTTCAATCCAAATCCAAATCCTCCATCCAAATCCCTAATCTGTCGATTCAATCCACAGAATAAAGATGATGATATACAACCGAAGAGATGGAAAGCTCTGGTTTCAACTGCATTGGCAGCTGCTGCCGTGATTACCTTCACCACCTCTAATAATATGTCTGCCTTGGCTGATCTTAATAAGTTCGAGGCTGATATTCGAGGTGAATTCGGCATTGGTTCAGCTGCCCAATTCGGTTCTGCTGACCTCAGGAAGGCAATTCATGTGAACGAGAATTTCAGGAGAGCAAATTTCACATCTGCTGATATGAGAGAATCAGATTTCAGTGGTTCAACATTCAATGGTGCATATCTTGAGAAAGCAGTTGCTTACAAAGCAAATTTCACAGGGGCAGATCTGAGTGATACATTGATGGATTGCATGGTCTTAAATGAAGCTAATCTCACAAATGCCATCCTTGTAAGGACAGTTCTCACTCGCAGTGATCTTGGGCGTGCAGTTATTGATGGTGCTGACTTTAGTGATGCTGTTTTGGACCTTACCCAGAAACAGGCTCTTTGCAAGTATGCAAGTGGAACAAATCCCACAACAGGGGTTAGCACAAGAACAAGCTTAGGGTGTGGAAACAGTAGACGGAATGCCTACGGTAGTCCATCTTCCCCATTGTTAAGTGCTCCACCACAGAAACTCCTAGATCGTGATGGCTTCTGCGATGATGGCACTGGACTCT