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mature miRNA: TGAAGCTGCCAGCATGATCTGG
species: Cycas rumphii
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|158967200|gb|EX920949.1|EX920949 iy84d12.g1 Cycas ovule - normalized (NYBG) Cycas rumphii cDNA 3', mRNA sequence 

TGAAGCTGCCAGCATGATCTGG
||||||||||||||||||||||
ACTTCGACGGTCGTACTAGACC

EST sequence (targeted fragment marked with yellow):
TCAAAGTAAATAACATCAAGCATTTAACAAAAATTATAAATATATACGGCAATAAGAAATGGTAAATGACACGCAAAATTTGTGCATTAAGACATCATACCATTTCCTGTTGAATGCGCTCAGAAAATTAGTCAAATCTCTAAAGCTGCAAATAACTTTACAGTTCTAGAGGGCTTCAAACAAATTTCTTTTGCATGACATATCTGTTTACAAAAGCCAAACAAAAGACGTTTTGGGCAGCTTACACATCATCACATGCTGTTAATCTTGCAACTCAATGAACGCTACGGGACTTCACGGGCAGGCTCCAAAGTACCAGTTTAAAAGCGATGAAAAGGACCCTTCATTTAATTGTATCGCATCTACTGCTCCCAAACTTCATGGTGTGCCTCCACGCGGGCTTTGCGTTATTAACTCCAGAAGCGAGTGGTCCACGAAACCATCATTTCCCTCCGAACCCCAAGCTGCGTACACCTGCATTTAGCGTCAGGGACATCCCTATTAACATTTAAATAAATAAATAAACATTAGACATCATCATTTCAACATGGACAGACGCTTGATGAATCTGAAACTCCAGAGGTGCTGCGTAAATTGTTATAATAATGTCTGCAAGGGAGTTGCTGAGACTGCCACATGATCTGGTACCCAAAAGCAACTGGCGAGAGCAATATTAACCAGATCATGCTGGCAGCTTCAGCCTCTCCCACGAACACGAACAAGATGCTCC


gi|66986045|gb|DR062479.1|DR062479 iq18c01.g1 Cycas ovule (NYBG) Cycas rumphii cDNA 3', mRNA sequence 

TGAAGCTGCCAGCATGATCTGG
|||||||||||| ||  ||||.
ACTTCGACGGTCCTAAAAGACT

EST sequence (targeted fragment marked with yellow):
CTTGATGAAACAATGGAATGTGTTTTTGTAGGCTTTAGAGTCTCCCATCATTCTTGACTTCACCACATCAACTGGGGAGCCAATACAAACTGCGAAGAACCCAGCTCCAAGTCCCGATAATAAATGAGTAAACACATTGTCAGAAAATCCTGGCAGCTTCAATAGAGTCTGTTTCACTTGATCATAACTGGCCAGCTCTGCAGCATTAATTATAGCATTGCGTGCCACATTTGGACCAAGTCCTGTCCACAGAGCACCTAGCCCTTCCTCTCGGACAATTGTAGCATATGCATTCATTGCCCCTGAATATCGCCTTGGCACTCCAGGTGGTAACTTCCCTTCAGCTTGTAGTCTCACTTTCACAAGATCAGTGGGGCTAGCCACTGTGATTGCTATGGCACCTGTTGTAAGGGCTGCAAGAATCTTCTTATGTAAAGGAACGTCCCCAACAAAATCTTTTCCAACATACAAGCTTTTAACCGGTTCATAAAGTCCAATCCGGAGTCCTCCGAACAGACACTGCCGATGCAGGCCGGGGACTATACCCTTCCAAAGAGCTGTTAAGCCTTCTTCTCTTGCGATGGTAGCAACCTTTGGATCCA