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mature miRNA: TGACAGAAGAGAGAGAGCAC
species: Cycas rumphii
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|27917896|gb|CB093704.1|CB093704 ze44d08.g5 Cycad Leaf Library (NYBG) Cycas rumphii cDNA clone ze44d08, mRNA sequence 

TGACAGAAGAGAGAGAGCAC
||||||||||||||||||||
ACTGTCTTCTCTCTCTCGTG

EST sequence (targeted fragment marked with yellow):
TTTGTCCGCCCACAATAGATGTTGCATCCGCTACCCATGGTTTATCGGGAGTCTCAGACTCTGGTTGTGCTCTCTCTCTTCTGTCATCCCAATCTTGGAGTTCAAGTGCTCCAGGGACAGCTTCTTTTGATATAGCTACACAAGGTGTCACAATGGATCAGCTTATACATGATGATCAACATCTCATGGCCCAACCCTTGGTGCAAGGTGTGCAGCACAACTTTGCAGACNAGTTGCTAACTCTTCGCTCCCAATCGTCGACAAGTCTAATTCCAAATGGATTTACTTCAGCTGCAGTAAATTCAATAAGTAAGGAGCAGCATGGAGAACCTATTGTTTCTACGCTAAACGGTATTGGAAACTTTGAAGGCCATGTATTTGGCTTGATGCAGGGAAACAATTTCAGTGGTTCTCAGGCAGTGAATTCACGAGATATTCAATGCACCATAGATTTAATGTGCAGGTCTACACAGGCACAAACCGATGAGTGCCAAAGTCAGCCTGGTATTGGGAACCAGGGAAGCAGACNATTCACCGACTTTCAGTCTTTGAGATCTTATGAATCATCAATTTATGACTCACAGCAGATGCTCTAGCTCTGACCATGTTTTGGCGTATGGCATTACCGTCTTGCCTTC


gi|158970041|gb|EX923405.1|EX923405 la78a06.g2 Cycas ovule - normalized (NYBG) Cycas rumphii cDNA 3', mRNA sequence 

TGACAGAAGAGAGAGAGCAC
||||||||||||||  |||
ACTGTCTTCTCTCTAACGTA

EST sequence (targeted fragment marked with yellow):
ATAAGTGATCCATGTCATTTGTTTCTTCAATTAACCCCCAACGAATTCACTACATGTGAAATCTGAAATGGATTTCATGTTTGCATAAATAAAACTATCAAGATTCTGAAATTAAAAATTCTTGCAAGACCTTCGACAGCTAGTTCTCACAGATGGCCTTTGCTTTCCCAGTCCTCCAAGATATATATAGATATATATAGATTTGGCATATATGTAAGTATCAAAAAGGCATCTTTGCACATGATGCCTGACAAAAGCTAGTAACTTTCTGTTCTATTTATGCAATCTCTCTTCTGTCAACTAGCTGCTGGCACCTGCAATACATTGTGTTGAAATTCCATTGCTTCATTTTCCTTGCATTGTTCCCAGTTCCTAAACAGGCTGAAGTCTGAAAAACGACAGGAAACCAGAATAATCCTAATCTAAAATGTAGAATTTTCTAAAGAAAGAAATATTTAAAAAATGGAATATTGAAAGTTAATTGAACACACTCCCGCCCTGTGAGTTCCACACCAAACAATCCTAGCGTTAAGCTACCATCTGATCCAGCTGCATCTGTTCAATCCAAGCACCCAGAACTGAGTGATCAACATTGTCCATCTGTCCTTTGTTTGATGTTTCTCCAGAACAACCTACAGTGCC


gi|158968075|gb|EX921534.1|EX921534 iy91c04.g1 Cycas ovule - normalized (NYBG) Cycas rumphii cDNA 3', mRNA sequence 

TGACAGAAGAGAGAGAGCAC
|||||||||||||| |||||
ACTGTCTTCTCTCT-TCGTG

EST sequence (targeted fragment marked with yellow):
ATCAACACATGCCATATGGTACTCATGATGACAGGGCAAAACCCTAAATCTTATCTCCCTCTTCATATTCTGCGAGACAAATGTAGCACCGGGCTGCTGCTGCTTCATTTACACTCTCATTCTTTTCAGGGTTCCTGTGATTCCGAAGAGGAAAGGAATCAACGACAGATTCTGGTGCTGGCAGCAACACAAGAGATAACGATGTTGACTGTGAAAGAGCAACCGACTGTCGATGAATTTCATCTAGCACCTCAAACAGAGCCTCTGCAAGCATAACTATACGTGAAATACTTGCCCATGTGCTTCTCTCTTCTGTCATCACATATGCCTCGCAAGAGCAACGGCCATCTTGATGGAGTCCAGATGCACAAAGTCTTGAGGCCCCAAAGACTCCATCAGAGTTATCACGCAATCTTTGCAAAGCCCAGACCTGCAGCACCAGAAGTTGTTCATTTTAGCAAAATTATCAAAATTTTAAAAACAATCACGGTCCCCAGGTTATTTTGGCGACAGTTGTGTTTTCACAGTAACTCTGCAAATCATGGGGGGGGCATGTTTTGAAAGAAAAATGACAAGACGGCATCGCCTCCTTGGTACTCAAATCTGAGAAATAAGGGTGGGAGTACCACTTTCTTACCTCACAAATTAGCCCGCTTCGCTTACCAAAGTTAAATAAAGC