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mature miRNA: AGCCAAGGATGACTTGCCG
species: Eucalyptus globulus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|162327402|gb|ES588994.1|ES588994 000001480252_D05.ab1 Eucalyptus globulus under low temperature Eucalyptus globulus cDNA, mRN

AGCCAAGGATGACTTGCCG
|.|||||||||| | |||
TTGGTTCCTACTCTAGGGC

EST sequence (targeted fragment marked with yellow):
GGGACCTTTAGACAAGTTTCGGTCCGGATTCCCGGGATCTCATCCTTGGTTTCATCATCGTTCCTGAGGAGTCTTCTTCTTCTCCTTCGTCTTCTTCTTCTTCTTCATTGCTGGCCTCAGCCTCGTCTTCTGCGGTTGAGAATTGGACCGGTCTTTTTTGTTGTCGGTGAGTTCTTCGTTCTCCTCTTTCGCATTCGCAGAAGCTTAAGATAATGGAGGGGTTATGTAACGCCGCCCTCCCTCGCCAACTGTCGTCAATACCCTTCACTCTTAGCCCTGCAAAATCGCTTCTTGCTCTACCCTTCTCGAATTTGAACTTGAATTCGAGAAGTGGGTTTCTCCACCCGAAACCCGAGACGGGTCCTTCGTCAAGAAACTGCTCGTGCTTGAGTGTGAGCAGGAGAGATGATGAGGATGGTACTGCCACCTCCATTGATGTTAAAGATGACTGGGAAGTGGAGTTTCTTGGGGAGATTGACCCTCTGGGCTTTCAAGCCCCTAAAAAGAGGAAGAAACGGGCCAAGTCTAAGCTGCTGCGGGACACTGATACTATGGAGTGGTGTGTGAGGGCTAGAAAGATTGCTCTCAAGGGGATTGAGGCTCGGGGGCTTTCTAGTACTATGGAAGATTTGGTTTCCGTTAAGAAGAAGAAGCAGAAGAAGAAGAGAAACAGCGGTAAGAAGCTCAAGAACAAGTCGAAAACCGATAAAGAGGATAAAGAGGACGTGATTGACAGCGATGAAGAATGGGACGTGGATGTGGATGAGGATGTGGAATTGGAAGACATCGAGGAATTGGGGATCGA