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mature miRNA: GTGTTCTCAGGTCGCCCCT
species: Fragaria vesca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|89541205|gb|DY667138.1|DY667138 STRB636JX cold-stressed Fragaria vesca seedlings cDNA library Fragaria vesca cDNA clone STRB6

GTGTTCTCAGGTCGCCCCT
||| ||||||||||| |||
CACCAGAGTCCAGCGAGGA

EST sequence (targeted fragment marked with yellow):
AAATGATAAGATGAGGAATGTAGAGGTGTGTATATGTTCACAGCATAAGCCAGCCACAAAGGGTAGAGGAGTCCCTGAGGAGCGACCTGAGACCACATGTGTGCACCCTCACCACACTCCAAAGTGAGAGTACATAACCACGTGTTCTCAGGTCGCCCCTGCGAGACTTTCCGTTTACCACATAACCGCGGCGATTTGGTGAGATGAGCAAGGAACAGATGTAGGTAAGTTATCTTTCATCTTCCCTGTCTTAAATAATCTGAATAAGATTACCTGACCCTGTTATTCAGGATTTGAGGTGATGAAAGTGTGACCTGCTAATGTATTCTATTTTGTGCTTACATTAGATTTGATCAAAATTTAACAGTAAGCATAGAAGTAGGAGATACAAAACCATGTCCAATTATGGTAAAGTTGGTAATCCTCATTGTTTTTGGAAATTCCAGTGACTCAAATAGTATAAGAGATAACTAAGCAATCAATCATGATGCTCATTGTTTCTTGTTTGCTCTCACTTGTCCTAATTACGAAGGCAATAAAGAGAACTACTTACTAGAGTTCCAAGTTTGTAACACTAAATGCAATATAATAATCCAGCATCATGATCTTCATCGTTCCTGCTTTATCTTCCCAAGTAAAACACTTGTTAGAGCAGAGTCACA