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mature miRNA: TGTGTTCTCAGGTCACCCCT
species: Helianthus petiolaris
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|90495613|gb|DY957471.1|DY957471 CHPZ534.b1_L13.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPZ534, mRNA s 

TGTGTTCTCAGGTCACCCCT
|||| ||||||. ||||||.
ACACTAGAGTCTTGTGGGGG

EST sequence (targeted fragment marked with yellow):
GTGCTCATTCACACACATCATCAGTCGATTTGCTTTGCAGGGGGTGTTCTGAGATCACATACAAACATGGTGAAGGCGGTTGCGGTTCTTAGCAGCAGTGAGGGTGTTAGCGGCACCATCTTCTTCAATCAAGAGGCAGAAGGTGCTCCCACTACTGTTACCGGAGACCTTTCTGGCCTCAAACCTGGACCTCATGGGTTCCATGTTCATGCACTTGGTGACACAACCAATGGGTGCATGTCAACTGGTCCTCACTACAATCCTCATGGAAAGGAGCATGGTGCCCCCGATGATGAGGTTCGCCATGCCGGTGATCTTGGCAATGTCACCGTAGGCGAAGATGGTACTGCAAAATTCACCATTGTTGATAAGCAGATTCCACTGATTGGAGCTCAATCCATCGTTGGGAGGGCAGTAGTTGTTCATGCTGATGCTGACGATCTTGGAAAGGGAGGCCATGAGCTCAGCAAAAGCACAGGAAATGCTGGTGGAAGAGTTGCATGCGGTATCATTGGATTGCAGGGTTGATCATATGCCAGGAAACAATCTGACTTCGACTATCCTCAGGTGTTGGTATTACTTCGTTTAAATAAACCGATGCTCTGTGTTAGTTGAATACTCCGATTGTATTTCGAGTTGAGACGTGTTTTGTGCAATGACGAATACTTTGTTTGGGTTTTTGAGTCTGTAGACCAAAGTTTATAATAAAAACATATCACCTTATTANAAAAAANANAAAAAAAAAAAAAAAAAACCTTGTC


gi|90480579|gb|DY942437.1|DY942437 CHPY10283.b1_F04.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPY10283, mR 

TGTGTTCTCAGGTCACCCCT
||||||||||| |||||||
ACACAAGAGTC-AGTGGGGC

EST sequence (targeted fragment marked with yellow):
TCCACCAACACACACACAAAAAGCAGTCTACTTTTCTTGATCAATCACATATTTCTGATCATTTTTGTGTTTCTTGATTCGATCTCTGTTCTGGGTGTGGAGTTTCGTTGAATCTTTGAAGGATCACATACAAAAAGATCATAAAAAATGGCTTCATATGGTGGAACAACACAAAAGTGTAAGGCTTGTGAGAAGACTGTGTACTTGGTTGATGAACTCACTGTTGATAATAAGGTTTATCATAGGGCTTGTTTCAGATGCCACCATTGCAAGGGTACCCTTAAGTTGAGTAATTACAGCTCTTTTGAAGGAGTGTTGTATTGCCAACCGCACTTTGATCAACTCTTCAAAATGACCGGCAGTTTGGACAAGAGTTTTGAAGGTGCCCCCAAAACGGCTAGATCATATGAGGGCCAATCTAACAGCAAAGTATCGAGTATGTTTGGTGGGACCCAAGACAAGTGTATAACTTGCAAGAAAACCGTCTACCCGCTTGAAAAGGTGGGTGTTGACGGAGGTGCATACCACAAGGCCTGTTTCCGATGCAGCTATGGCGGGTGCCACATAAGCCCGTCAAACTATGTGACACATGAACATCAGCTTTTCTGTAAGCATCATCACGCGCAGCTCTTTATGGCTAAGGGAGATTTTAGCAAATTTGATAAGCAACATGAACCGGANAACGGGGTGACTGAGAACACAACAGAGGTTTGATAATTTGAGGTCAGATTCGACGTGNGATTTCTGGAAATCTTGAAAGTATGCAGAGCAGAGGTTTTGCCAGTCGTTTTGTTGTGTTGTTGAAAAGAGATGTGTGCTTTTTGTCTGCGCGTGT


gi|90491210|gb|DY953068.1|DY953068 CHPY9375.b1_N15.ab1 CHP(XYZ) plains sunflower Helianthus petiolaris cDNA clone CHPY9375, mRNA 

TGTGTTCTCAGGTCACCCCT
| ||||||||||  |||||.
AAACAAGAGTCCTCTGGGGG

EST sequence (targeted fragment marked with yellow):
CGGGGGTCTCCTGAGAACAAAACAATTCAGATCCATTATCCCTCTTCAACCCCACAATCAACCCTCATTAGGGCTTTTCGGTTGAAGGGGCACAAATTAACTCTATTCAAGTTCTGGTGTCCGTTGATGATAAGAAGGTTATACGAGCAACTTATCGTCGAANATGAGGCAACGGAACATGGCTTA