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mature miRNA: GTGTTCTCAGGTCGCCCC
species: Helianthus tuberosus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|125439067|gb|EL450764.1|EL450764 CHTM3777.b1_B10.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTM3777, m 

GTGTTCTCAGGTCGCCCC
|||.||||||||  | ||
CACGAGAGTCCAAGGTGG

EST sequence (targeted fragment marked with yellow):
CAACTGAGAGAATTGGCTTCATTGTTTACTCGATTGGTAAGCCGCTGGATGCGTGGCTCAAGGACATGCCTGCTTTAGTTTAAAGAAAGTATTCGGTTACGTTGTTTGGTTTGTGGTGGAACCTGAGAGCACTAGTCAGTTCAAAGTCTTCACTATTTTTGGCTGGTCAGTTCTTTATTCTTAAAATTGTTCTTTGTAGCGAAAAAACAATCATGTGGGTTTGCCATATATGTTTACTAAGCTTTACATTCCTTCTATGTTGTACTTATTGATATTGTTACATTTTTCAACTTCACCGGATTTTATTTTGTTTTGTTCAACACTTGTCAAAATAGTTTTAAGTAATGTGTAATTTGAGCAAGCATTCTTGTTAATGCATCATGGTGTTGTACTAAAA


gi|125455086|gb|EL466779.1|EL466779 CHTS5577.b2_A04.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTS5577, m 

GTGTTCTCAGGTCGCCCC
||||||.||||||.||
CACAAGGGTCCAGTGGCT

EST sequence (targeted fragment marked with yellow):
GCGCAGCGTCACAACTACGGCCATTACAGTAGCACTCACCCTTAACTCCATTTCACCATCTTCATCCTCATCACACAACAATCACAACTTCACAAAACCTTTTTTCAATCAGCCCTTTTCATCCCCAAATCGTTTCGGTCTCAACAAGAATCTCACCATCCCCCCTTCTGCTCTTCACATGGACACTAAACAGGCTCCAAAGTTTGATGCGGAATTGCCGGAAGTTATGACAGAGTTCATGGTGGATATGAGTTGTGAAGGTTGTGTTAAAGCTGTCAAGAATAAGTTGCAAACAGTCAATGGAATTAAGAGCGTTGATGTGGATTTGAGCAATCAAGTAGTTAGAATTTTTGGGTCTTCACCTGTAAAGACCATGGCTGAAGCCTTGGAACAGACCGGTAGGAAAGCCCGGCTAATTGGTCAAGGGTTACCAGGAGATGTCTTAATTACTGCGGCTGTTGCTGAATTCAAAGGCCCACAAATATTTGGGGTGGTCCGTCTGGCCCAAGTGAGTATGGAATTGGTTAGAATCGAAGCCAACTTCAGTGGGTTGTCACCTGGAAAACACGGTTGGTCCATAAACGAATTCGGTGATCTTACTCGAGGTGCCGCCAGTACTGGCAAAGTGTTTAATCCTGTTAATCAGCTCTCAGAAGAAAAGGCGCTCGGTGACCTGGGAACACTAGAGGCTGATGAGAACGGTGAAGCGTTCTTCTCGGGTGTGAAAAAGAACCTTAAGATCGCGGATCTTATTGGTCGAGCAATAGCGGTGTATGAAAATGAAGACAGAGGTGATGCTGGGCTTGCGGCAGCGGTTGTAGCAAGAAGTGCAGGAGTTGGTGAGAAACTACAGAAGCTCTGCACCTGTGATGGCACCACCATTTGGGAAGCAACCAATGCTGGATATGTATCCAGTAAAGTTAAT


gi|125458663|gb|EL470356.1|EL470356 CHTS8986.b3_D15.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTS8986, m 

GTGTTCTCAGGTCGCCCC
||||||.||||||.||
CACAAGGGTCCAGTGGCT

EST sequence (targeted fragment marked with yellow):
GCGGGGGAGATGACATGGGTTTTCTTAAACAGTTGCAGCAGCGTCAACAACTACGGCCATTACAGCAGCACTCACCCTTAACTCCATTTCACCATCTTCATCCTCATCACACAACAATCACAACTTCATAAAACCCTTTTCATCCCCAAATCGTTTCGGCCTCATCAAGAGTCTGACCAGCCCCCCTTCTGCTCTTCACATGGACACTAAACAGGCTCCAAAGCTTGATGCGGAATTGCCGGAAGTTATGACAGAGTTCATGGTGGATATGAGTTGTGAAGGTTGTGTTAAAGCTGTCAAGAATAAGTTGCAAACAGTCAATGGAATTAAGAGCGTTGAAGTGGATTTGAGCAATCAAGTAGTTAGAATTTTTGGGTCTTCACCGGTAAAGACCTTGGCTGAAGCCTTGGAGCAGACCGGTAGGAAAGCCCGGCTAATTGGTCAAGGGTTACCAGGAGATGTCTTAATTACTGCTGCTGTTGCTGAATTCAAAGGCCCACAAATATTTGGGGTGGTCCGTCTGGCCCAAGTGAGTATGGAATTGGCTAGAATCGAAGCCAACTTCAGTGGGTTGTCACCTGGAAAACACGGTTGGTCCATAAACGAATTCGGTGATCTTACTCGAGGTGCCGCCAGTACTGGCAAAGTGTTTAATCCTCTTAATCAGCTGTCAGAAGAAAAGGCGCTCGGTGACCTGGGAACACTAGAGGCTGATGAGAACGGTGAAGCGTTCTTCTCGGGTGTGAAAAAGAACCTTAAAATCGTGGATCTTATTGGTCGAGCAATAGCGGTGTATGAAAATGAAGACAGGGGTGATGCTGGGCTTGCAGCAGCGGTTGTAGCAAGAAGTGCAAGAGTTGGTGAGAACTACAAGAAGCTCTGCACCTGTGAATGCACCCACATTTGGGAAACAACCAATGCTGATTATGTATCCCGTAAAATTT


gi|125429727|gb|EL441699.1|EL441699 CHTM18436.b1_G01.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTM18436, 

GTGTTCTCAGGTCGCCCC
.||||||||||||| ||
TACAAGAGTCCAGCCGGA

EST sequence (targeted fragment marked with yellow):
GAAGAGTACTACCCTATCACTTAACAAACCTTCATAACAGGGTTTGTGGGGGTAGGCCGACCTGAGAACATATCCTGGAGGCCCAACACAGGTCCCGATTAAAGGAAACCTCCACTTGCTGGGAGCGTTACCGCACCAGTCGCTAACGAAACTAGCCAAAATTCATGGCCCGATAATGTCTTTACAACTCGGTCAGATCACCACGCTCGACATATCCTCACCCACTGCCGCGGAAGAAGTACTCAAAAAAGCAAGACCTTGCTTTCTCCACACGTAATGTCCCTGACGCGGTGCGTGCCTATTACCACGAGCGACACTCCATCTCATTTCTCCATGTGTGCACGGAGTGCCGCACCCTCAGAAAGATTGTTAGATCCAATATCTTCTCCAACAGCTCTCTTGAACCCAAACACCACTTGACGAGCACGAAAGTTGAAGAACTCATATCATACTGCCGGAAAGCTGCTCTTTCTAATGAAAATGTGCACATCGGTCGTGCTGCATTTAGAACCTCGGTGAATCTTTTATCCAACACTATTTTCTCCAAGGATTTAACAGACCCCTATGACGATTCAGCTTCAGGTAAAGAGTTTAGGGAGGTGATTACCAACATTATGGTGGACTCTACAAAGACAAATCTAGCTGACGTATTTCCAGTGTTGAACAGGATTGATCCACAAGGGATCAAGAGAGGAATGGCTCGACATTTTTCCAACGTTCTTGGGATATTTGATCAGTTCATTGAAGAGAGAATGAGAACGGGCAGATTCGAACAATGTGATGTATT