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mature miRNA: TGACAGAAGAGAGTGAGCAC
species: Helianthus tuberosus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|125451091|gb|EL462787.1|EL462787 CHTS1727.b2_N23.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTS1727, m 

TGACAGAAGAGAGTGAGCAC
||||||||||||| ||||
ACTGTCTTCTCTCCCTCGCA

EST sequence (targeted fragment marked with yellow):
AGGGAGGGGGGTATGGATGATAAGTTTTAAATGAGATGATGGAAGAATGGATAGAGGATGATGATCATGATGATGATAGGAAGAAGGGTATAATGGGAAAGAAGAGTTCTAGTAGTAGTAGTAGTAGCTTTTGCCAGGTGGAAGACTGCTCAAGTGATATGAGTCGAGCCAAAACATATCATCGGAGACACAAAGTATGTGAGTTTCATGCTAAGGCTCCAATCGTTGTCATTAGTGGACGCCAACAACGTTTTTGCCAGCAATGCAGCAGGTTTCACGACTTAACTGAATTTGATGATGCCAAAAGGAGTTGTCGTAGGCGCTTAGCTGGACACAACGAGCGCAGGCGTAAGACCTCCTATGGAGAAACTTCTGGTTAATGATAATGATAACGATGATATTATTATCGCCAAATAAGACTTTCTTATATATATGTCCGGTAGCAGTAGCGGTAGCCACGCTCCCTCTCTTCTGTCAGTCCCTAAATATATCTATTTCAATGCATGGCCATACTTGTACTAAATAACGCAATGCTAATAAATTAAAGAAACCTTCC


gi|125435834|gb|EL447531.1|EL447531 CHTM2628.b1_H10.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTM2628, m 

TGACAGAAGAGAGTGAGCAC
|||||| |||||||.|| |
ACTGTCATCTCTCATTCTTT

EST sequence (targeted fragment marked with yellow):
GTTGAACTCATATCCTTCGGCCTTGACTGAACAAGCAAACACTCCAAACCGTCCACCCAAATGAACATCCAAATCCACCCAACCGCCAATTTCACACTCTCTTCTCGTAACCCCCGCATCTTCTTCCTCCCTCAATTCCCCCCAAACCCACAAAAGATCCCCAAACACACTTACGCCACCAAACCCCTGTTCAAAACCCTCATCAAATGCTCACAAACCAGTGATTCTGACCCACCAAAATGGGAAAAACTGCTGCCAAAAAATATTATCTCAGCTGACAAGATCCTCAGGTCCATTGCAGGGGCAACTTCTAGCCCAATTTGCCAGTTCATATCTTCACCCACCACTTTCTTGCACTCTGTTGATCCCAGAATCAAATTGGCATGGCTTCTGGTTCTAGTTGTTATCCCCGCAAGAACACATATATACATGCGATTCGGATTAATCACGTTTCTGGCCACATTGTCTGTATGGATACTGCCAAGACAAGTTTGGGTGGATCAATTGGGAAGAACCGCTCTATTATCTGGGATTTTGTTCTTAATGGTGGGGCTCGGTGCAGATAGTGTTCCAATGGTGGTTCAGTCAAGAACTCCACCGCCATCGATGATGGGCTTGCCGAATATTCCACCGTCTTTTGATGGTTATTCCTATCTACTGTTCAAATTAGGACCGCTGCAGCTTACGAGAAAAGGGTTGTCTCTAGCGAGTACAACAGCTTGCTTAACTTTTTCTATTTTTCAAAGTGCAAGTCTTTGCTTGACAACTACTACACCAGAACAACTCGCGTTTGCTCTACAAATGGTTATGAAGCCTTTGGCATATGTTGGTGTCCCGGTGGCTGAAAGTATTTCTTACTCTCTACTGTCATAAAGAATTATCAACCTAGTATTCGATGAAGTACGGATTGTGGCATTGG


gi|125459183|gb|EL470876.1|EL470876 CHTS9514.b2_D04.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTS9514, m 

TGACAGAAGAGAGTGAGCAC
..||||||||||..|||||
GTTGTCTTCTCTTGCTCGTC

EST sequence (targeted fragment marked with yellow):
TCTTAGAGTCTCAATTTGCCGCCTAAATCTGAGAGGACAAATGAGACGTCAAGTAAAGACATCTTAGCTAAGCCGTATGTTGAACCTAGCGATGAAAATGTTGGAGAAAAACAAAGGAAAAACACTGTTTCTTTGGTTGAACCGCCTGTGAATGGAAGCGAGGTTGGACATCCTGGCACAGATGTGGTGGTTGCAGAAGTTGAATACATCGAATCTGACAAACTGAATGATCTAGATGATATTGATGAGTGTCTTAAGGTGCTTTTAGATGGGTTAGGGTCTAAAGACTGGGTCTTGCTTTGTGATACACTCAACAATGTACGTCGCTTCTCAATATACCACAAGGAAGCATTGCAAGACATATTGGACAATGTGATTACCCTTGTTGTGAAAGCCCTGAAGAATCCAAGAAGTGCTGTATGTAAAACAGCCATCATGACATGTGCAGACATTTTCAAAGCATTTGGGGATACCATAGTTGATTCGTTGGATCCTTTGTTGGTACAACTTCTTCTCAAGTCTTCTCAAGACAAGCGGTTTGTATGTGAGGCTGCTGAAAAAGCTTTAATAGATTTGACAATTTGGGTCTCCCCTGTTTTGCTGTTACCCAAGTTGCAACCTTACCTTAAGAACCGCAATCCCCGTATTCGTGCAAGGGCTTCAATGTGCGTTTCTCGTAGTGTACCACGATTGGGTACCGAAGGAATCAAAGATTACGGGATTGACAAATTGACTCAAATAGCTTCATCCCAACTGAGCGACAAGCTCCCTGAGTCACGAGAGGCTGCTCGTTCTCTTCTGTTGGAGCTACA