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mature miRNA: TGCATTTGCACCTGCACCTTT
species: Helianthus tuberosus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|125437242|gb|EL448939.1|EL448939 CHTM27588.b1_H10.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTM27588, 

TGCATTTGCACCTGCACCTTT
|||||||||||||.||||| |
ACGTAAACGTGGATGTGGACA

EST sequence (targeted fragment marked with yellow):
GGGGAATGATTTAATGAGTCTATGGGGAATTAAGGATTTCTTGTGGATGTAGTATTGATTAATGAATGATACTGAATTGGAGTTTGCTGTGAGTATTGCTAACCAATTGATGAAGAACACTAATACAGGTGTAGGTGCAAATGCAAGTTCAAAGGCAGATATGATGCAATTTGTGGGGTCTGATACATTAGCGGAGCTAGTTTGGTCTCCAAGAACAGGTTTAAACATCAAATTTGCTGAAGAAAAACCTTGTTTTATGTGGGAAGTTGGTCCAACTGATATGAGCTTATTGAGCTCTGAAAACACTCAAAATGAACAACACATTGCATCACTAGCAACATGCCATGTTAGTTGCACAGGTTCACAAAACCAAGGGGAGGTTGATAAGTTATATGAACATTTATTTGAAGACGGAATGATGGGTGGTTGTGACGGGTCGGGTTCCAAAAGTAGGTCACCTTTGAAAATTGAACCAATGATGCTATATAGGCCCGAAAAACATGCCGAAACGGATAACGTTAAAATGGAAAAAGATGGTTACTTAAGTGACCCTTTTCTTGAAAATGCCCAAGTTGCAATCTTTGATAACAAAACTGCTGAGAAACACCAAGTTGAGAGCCTTAGAAACATGGGAAGTTGNCAAAGTGCTAATTTCTTGACCGAAAGAAAGAGGAAATGCGGGTTCGATCAACAACTGATTTCTGGAAAGTAACGAATCAAGAATCAAGTCGATGGTAGCTCGTTTATGAACTGGATTTCAAACACTTGAAAGGGATTAGGGACAACCATAACGTTTATG


gi|125454004|gb|EL465697.1|EL465697 CHTS4583.b1_N18.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTS4583, m 

TGCATTTGCACCTGCACCTTT
.|||..||||||||||||| .
GCGTGGACGTGGACGTGGACG

EST sequence (targeted fragment marked with yellow):
CTGTTCTTGCACCAAAGGCTAATAATAACGGGGGTCCATCAAAGCGTAATAACAACCCTCTTAAAGAGGAACCCGTCGACAATAGCCCCTCAAAGCCAGCCGCTGCTACAACATCAGCTCGGGGAACCCCATCTTCAAACAAGTCGGCTAAGGGAAAGCGAAAAAGCACCGAAGAGAACAAACCGTCAAATGGTGCCGCCACAGCTTCAAAGAAAGTCAAAACCGAAAATGACGTGAAACATGTAAAGGAGGAGCCTGCAAAGGCCGGTAAAGGTTCTTCTTCTAAACCAGCAGGTGCAGGTGCAGGTGCAGGTGCGTCATCTGCTACCGGACCTGTCACGGAAGAAGAAATTTCGGCGGTTCTGCTGCACAACGCACCTGTCACCACACAGGATCTTGTTGCTAAGTTTAAATCCCGCTTACGCACCAAAGAGGACAAAAACGCGTTTGCAGATATTCTGAGAAGGATTTCCAAGATACAGAAGACCAACGGTGCCAACTATGTGGTGTTGAGAGACCGATGAGGTGCTGAGAGAAACGGGCCCCGGCCCTTTACCCGACCCAGCTGTTTTATATACATCGATACTTTACTTGACGTCAACTGGATTCCTTTCCCTTCCCTTTGCAGATGACTTTTGGTAGATGTTGCTTGTGCTTCATTGTCATTTGAATCTGCACTTGTTTCAGATTTATGTATTATGAGTCCTGTTTACTATAATGATCTGTTTTCTATTAATAAGTGCAACAATCGGGCTGCTTTTAATCCA


gi|125454004|gb|EL465697.1|EL465697 CHTS4583.b1_N18.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTS4583, m 

TGCATTTGCACCTGCACCTTT
||||..||||||||||||| .
ACGTGGACGTGGACGTGGACG

EST sequence (targeted fragment marked with yellow):
CTGTTCTTGCACCAAAGGCTAATAATAACGGGGGTCCATCAAAGCGTAATAACAACCCTCTTAAAGAGGAACCCGTCGACAATAGCCCCTCAAAGCCAGCCGCTGCTACAACATCAGCTCGGGGAACCCCATCTTCAAACAAGTCGGCTAAGGGAAAGCGAAAAAGCACCGAAGAGAACAAACCGTCAAATGGTGCCGCCACAGCTTCAAAGAAAGTCAAAACCGAAAATGACGTGAAACATGTAAAGGAGGAGCCTGCAAAGGCCGGTAAAGGTTCTTCTTCTAAACCAGCAGGTGCAGGTGCAGGTGCAGGTGCGTCATCTGCTACCGGACCTGTCACGGAAGAAGAAATTTCGGCGGTTCTGCTGCACAACGCACCTGTCACCACACAGGATCTTGTTGCTAAGTTTAAATCCCGCTTACGCACCAAAGAGGACAAAAACGCGTTTGCAGATATTCTGAGAAGGATTTCCAAGATACAGAAGACCAACGGTGCCAACTATGTGGTGTTGAGAGACCGATGAGGTGCTGAGAGAAACGGGCCCCGGCCCTTTACCCGACCCAGCTGTTTTATATACATCGATACTTTACTTGACGTCAACTGGATTCCTTTCCCTTCCCTTTGCAGATGACTTTTGGTAGATGTTGCTTGTGCTTCATTGTCATTTGAATCTGCACTTGTTTCAGATTTATGTATTATGAGTCCTGTTTACTATAATGATCTGTTTTCTATTAATAAGTGCAACAATCGGGCTGCTTTTAATCCA


gi|125440021|gb|EL451718.1|EL451718 CHTM4584.b1_P18.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTM4584, m 

TGCATTTGCACCTGCACCTTT
||||||||||||||||||| |
ACGTAAACGTGGACGTGGACA

EST sequence (targeted fragment marked with yellow):
GCTTCGGCGGGGCCATCTTCATCAGTCTGGCATAGATGCATGATGTTATATATATTTGTATAAAGACGTCTGGCGGTTCTATACATCCCATTCTTTTTCATTCATTCTATTTTTAGAAATCCTCCAACACTTTAGTGACCAAAATTCAGCAGTCACTCAAAGAAAACTTTTGAATAGATTTAATGAGTCTATGGGGAATTAAGGATTTCTTGTGGATGTAATATTTGATTAATGAATGACACTGAATTGGAGTTTCCCGTGAGTATTACTAATCAATTAAAGAAGACCCCTAATACAGGTGCAGGTGCAAATGCAAGTTCAGGCAGATATGATGCAATTTGTGGGGTCTGATACATTAGCAGAGCTAGTTTGGTGTCCAAGAACAGGTTTAAACATCAAATTTAATGAAGAAAAACCTTGTTTTATGTGGGAAGTTGCTCCAACTGATATGAGGTCTGAAAACACTCAAAATGAACAACATATTGCATCACTAGCAACATGCCATGTCAGCTGCACAGGTTCACAAAACCAGGGGGCAGTAGATAAGTTATATGAACATTTATTTGAAGATGGAATGATGGGGGGTTGTGACGGATCGGGTTCCGAAACTAGATCACCTTTGAAAATTGAGCCTATGATGCAATATAGGCCCGAAAAACATGCTGAAAGGGCTGATGCTGATACGCATAACGTTAAAATGGAAAAAGATGGTTACTTAAGTGACCCTTTTCTAGAAAATGCACAAGTTGCAATCTTTAATAACAAAACTGCTGAGAGACACCAACTTGAGAGTCATGGAAGCATGGAAAGTTGTAGAGCTAACTTCTTGACCGAAAGAAAACGGAAATGCGGCTTCGAACAGCAACTGATTCTTGGGAGTAAACGAATCAAGAATCAAATCGATGGTAGCTCGTTCATAAACTGGATTTCA