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mature miRNA: ATTAGTCCCGGTTGGTAAATA
species: Hordeum vulgare
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|21150068|gb|BJ471565.1|BJ471565 BJ471565 K. Sato unpublished cDNA library, cv. Haruna Nijo adult, heading stage top three lea

ATTAGTCCCGGTTGGTAAATA
||||||||||||||||| .|
AAATCAGGGCCAACCATT-GT

EST sequence (targeted fragment marked with yellow):
GGCTAGGCTTTAGTCCCGGTTTACTAGGAACCTCTAGTCCCGGTTGGTGTTACCAACCGGGACTAAAGGGTGGTATCAGGGTTTTGTCAGGCTGGGGCCCATGCGAGGGCAATTAGTCCCGGTTGGTAAATACAAATCGGGACTAGAGATAGATCTTCGGTCCCGGTTTATATCACCAACCGGGACTAAAATTATCACTATAAATAGTGCTTCGTCCAGCCCGAGCCCAAGTTCTCTATTTTCTCCTTTCTCTTCTGTGTTCTCTTCCCTTGCTCGAGTTCATCCTCAATTTCTTCCTAGATTTGTGAAGATTTGAGGCACCCCATCCATCCAAGTGTTCACAAAGGTTAGCAACTTTGTCCTTTCATCTCTCATCCCCGTCCTAACCACCATCGATCGCCCTCATTGTCTCGTCGCTGGCACCACCTTGGTGATCCTCTTGTTCTTATCTTTTTATTCCACAAAAATCTTCTTTGGATGATTTAGATAGTTTTCTTACTTGTATGATTGTTTGTTATACATAGTGCCATGGTCTTGATAT



gi|94349949|gb|BY861994.1|BY861994 BY861994 Etiolated seedling shoot Hordeum vulgare subsp. vulgare cDNA clone baet40a02 3', mRN

ATTAGTCCCGGTTGGTAAATA
||||||||||||||| ||.
AAATCAGGGCCAACCACTTGG

EST sequence (targeted fragment marked with yellow):
GATGGATAGGAGACCACTAGGCATCCTCTTACCTTCTCCTTTCCTCCCTTGTTTCTTCTTTTCCGGTGACTCTCGCCGGAGTTGTTTTCCTTCACTAGTGGGGACGGGGCCTTTAGCCCCGGCCTGTAAGGGGCTTTAGTCCCGGTTCACCAACCGGGACTAAAGGGGCGGGACTAAAGGCCTAACCTTTAGTCCCGGCCCTCTTACAATCCGAGACTAAAGGTGCTTCACGTCGGCGCCTCGTAGCGCCCCAGAGGCAGGCCCTTTAGTCTCGGTTCGTTACACGGACCGGGACAAAAGATTTTCAGATTTTGCTGGTTTTTGGGTTTTTTTTTAATGAAATTATTTTTGGGTTTT