miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: CGGGTCGGCGCTCCACGCGGC
species: Hordeum vulgare
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|21944137|gb|BM101325.2|BM101325 EBpi01_SQ003_I13_R pistil, 1 DPA, no treatment, cv Optic, EBpi01 Hordeum vulgare subsp. vulga

CGGGTCGGCGCTCCAC-GCGGC
|.|||||||||||||| ||.||
GTCCAGCCGCGAGGTGGCGTCG

EST sequence (targeted fragment marked with yellow):
GGCCCCGCTGCGGTGGAGCGCCGACCTGACGGCGGCGGCGGCGTGGACCGCGTCGCAGCAGCAGAAACAGAAGAGCTGCGCGTTCGCGGACATGGGCGCCAGCCCCTACGGCGCGAACCAGGGGTGGGCGAGCTACCGCGCGCGCCCGGCCGAGGTGGTGGCCTCCTGGGTGGCGCAGGGGAAGTACTACGCCCACGCCAACAACACCTGCGCCGCCGGCCAGCAGTGCGGCACCTACACGCAGGTGGTCTGGCGCCGCACCGCCGAGGTCGGCTGCGCGCAGGCCAGCTGCGCCTCCGGCGCCACCCTCACGCTCTGCCTCTACAACCCGCACGGCAACGTCCAGGGCCAGAGCCCCTACT



gi|166068511|gb|FD518197.1|FD518197 RUS52F02w HB (Hordeum vulgare cv. Barke) Hordeum vulgare subsp. vulgare cDNA clone HB30J07,

CGGGTCGGCGCTCCACGCGGC
|||||||| ||| || .||||
GCCCAGCCTCGACGTCTGCCG

EST sequence (targeted fragment marked with yellow):
AGATGGAATTTGCTTGTGTTGCTTGATAAATATTGCTGCTTCCGATTCTAATGTTGCATGCTTATAAGTTTGAACTATCCAATGTTACGTGGTCAAGTAGCAATAAACTGCACAATGCCATGTCATCGCAACACTGCCGCTACATCAGTGAATTTCCTGTTCTACAGGTTTTAGGTGGATTCCAACAGGAGATATGGAATTTCAGATAGCAAGTCTGAGCCCATGATTTTCAGCAAGTCTTAACCACGACGTTCAAGAGTGACTTGAAAAGAAAACCCTCCATCATCAAAAGTATATGTGTCTCATTCTCCAAGGCAATGGTAACGTAACGTAGCCAATGCAGCCACTCGCACCCACAGTAACTTCAAAGCATCATCTGCCAACTCCTCAAAGCCCAAGGAGCATACAATCATACGCACATGAGCCGTGACTGCTGAATGTACTGATCGCTGATCATACGTTCGAGGCGTAGCCATGGGCGCCACAACCAACCATGCCGCTCCCATCTCTCGAGCCATCCTTTCGCTCGTGACGATCATCTTCTTCTCCGCCGTCTGCAGCTCCGACCCGGACCTCCTCGTCGACTACTGCGTCGCCGACATGAGGGCCGCCGCCGGCGCGCACCGGCACAGCTACCGCTGCCACCCTCCTCCATTCTATTTTGTTCCCTTGCGCCAAG



gi|21931240|gb|BM442148.2|BM442148 EBan01_SQ002_F15_R anther, yellow stage, no treatment, cv Optic, EBan01 Hordeum vulgare subsp

CGGGTCGGCGCTCCACGCGGC
||||| ||||||| | .||||
GCCCAACCGCGAGCTATGCCG

EST sequence (targeted fragment marked with yellow):
TAGAGAGTTAGTTGTCCATGGATCGGGGCCTCCAATGGAGCAAGCCGGCGTCGCTGCTCCTCTTGGGGGCGGCGTTCCTGGCGACGGCGGCCGTATCGAGCGCCAACCCGGCGTACAACCCCGACCCCTACAGCGTCGTCGACAACTTCAACCGCGCCGTCCACAGGTCGACTAGCCCGCGTCGTGCGCTGTCGTCGGAGAAGAAAGAGAAGAAGCAAAAGTACAATGGCCCGTGCCTGGCGACGAACCCGATCGACCGGTGCTGGCGCTGCCGCAAGGACTGGGCGACGGACCGGCAGCGGCTGGCCCGCTGCGCCAA



gi|166066887|gb|FD517998.1|FD517998 RUS49G09w HB (Hordeum vulgare cv. Barke) Hordeum vulgare subsp. vulgare cDNA clone HB27H07,

CGGGTCGGCGCTCCACGCGGC
|||||||||||| ||||| ||
GCCCAGCCGCGACGTGCGGCG

EST sequence (targeted fragment marked with yellow):
AAACTCACTTTATTATTATATAAATTGGAAAGGAAATTTAAAAAGGAAAAATACATTAACCCTTGGCCAACCACTCAACATTTTCAAATGCTTGTGTTGTGGGCTTGTGGCCGTCCTCCACACATCTCATCATATAGAGATGCTGTTGGGGATGCCCCTAGCAGTGAGCCCCTCAGCCTTGCCCGTGTGGTCGGAGGTGTTGGGGTAGAGCAGCATGTATGGAAAGTGGACCGGTCCATTGCGGTTCTTGAGCTGGGGGTTTCCGTTCAAGGCGACCACCTCGCTCTCGATGCCCTCCAGCCTCGTGCCGAACCGCTTGAACGCCTCCAGCGCCTTGGCATCCGACGTCCACTCCGGCGTGTCACGCTGCCCGAGGTAAATCTCGTCGGAGGAGTGCTTGGATAGGATCTCCAGCAGAGAAATGCCAATGATGGCCTGCAGCTGGTTGGTGATGGTGCGGATGAACACCTTTTCTGGATCGCGGGCCAGCAATGCGTACTCCTCGGTGCCTGGCTCCGGCATCGGACGACGGCTCGCTGATGGCTTGTTGGGGTGGTAACCGGCGTACGGGTACTGCCCGAAGTTGACGGCGGCGTGCAGCGCCGACCCGGTCCAGATGATGGTGGTGCAGACCTTGACCAGCTCCGCCACCGTCTTCATCTCCGGCCACCATGCCGCGTCCTTGAGGTCGCCGTGGCCG



gi|217041607|gb|GH207975.1|GH207975 HV_CEb0006J07f2 Hordeum vulgare (Blumeria challenged) seedling green leaf cDNA library HV_CE

CGGGTCGGCGCTCCACGCGGC
|||||||.||| ||||.|| |
GCCCAGCTGCGCGGTGTGCGG

EST sequence (targeted fragment marked with yellow):
TGCAGGCCCCGCGCCACGCCACGCCGTCGCACGTGCGCCTCGTGCGCGGCGCGCGCGGCGACCGGGTGTGCGCGCTTTCCTGCTCCGGCCTCGACGTCGCCGGCGACCCTCTTGGGACGTCCGCCTGCGTCGCCATGAAGGCCCCCGGCGTGTGGCGCGTCGACCCGGTGCTGGCCGCGGTGGGCGACCGCGTGGTCGCCCTCGGCGGCGCGTGCCAGCTTGCGCTCGCCGAGGGAGAGGACGCCGCCGCGGTGGAGGTCCACGAAGGCGGGCACTGGACGGCCTGCGATCCGATGCCGGCGGAGCTCCGCGAGTCCGCCGCGGCAACGTGGCTTTCGGTGGCCGCCACGGATCAGAGAGTGTACCTCGCGGACAGAAGCACGGGCTGTGCGAGCTGGTTCGATCCGTCG



gi|24273335|gb|BU996352.1|BU996352 HM13F08r HM Hordeum vulgare subsp. vulgare cDNA clone HM13F08 5-PRIME, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
|| |||||||| |||.||||
GCGCAGCCGCGCCGTGTGCCG

EST sequence (targeted fragment marked with yellow):
CGGCACGAGGGCCGTGTGCCGCGCCGACGCGTCCCCGTGGGACCCGGAGCACGCCGCCTTCCGGCTCCTCGGCGTGAAGCCCGGCGGCGCGGCGGTCTGCCACGCGGTGACGGACGCGCTGGTCATGCCGGCCATGAACGGGAAGAGCCCCGCCGCCTACTAAGTGGCCCCTCGTGTCTGTCGTGTCCGACTCTGTATGACCGTATCCATACGGTGAACTCGTATCTCTGGGACCGTATAACGCTCATTGGTATAGCTGTTGTATTATACATGTTCATTCCACTTCCACTGTATGTGGCTAAGCACTCTATAGAGTGATGTATCACTGTTAAAATAAAGGATGCATGTTTCAAT



gi|94342206|gb|BY849345.1|BY849345 BY849345 Germination shoots Hordeum vulgare subsp. vulgare cDNA clone bast07d06 5', mRNA sequ

CGGGTCGGCGCTCCAC-GCGGC
|.|||||||||||||| ||.||
GTCCAGCCGCGAGGTGGCGTCG

EST sequence (targeted fragment marked with yellow):
TCGCGCGCGCACACTTCACTGTCCGCTCCACTCGCCCACGCTCGGTGCTCTCACTTCACTGCTCACCGCTCGCCATTACTGGCCTCACCCACCCCAGATCTCATCCATGGCTCCCCAGCGCCGCCACCACGCGGCGGCCACGGTATGCCTTCTCGCCCTCCTCGCCCTGTCCGCCGCGCCGGCCTACGGCAGCCGCGCGCTGCCGGCCAGCAAGGGCGCGGCAGCCAGCAAGGCGCAGGCGGCGGCGGCCAACGCGACGGCGACGGCGACGGCGGACGAGTTCCTGGCGCCGCACAACAAGGCGCGCGCGGCGGTGGGCGTGGCCCCGCTGCGGTGGAGCGCCGACCTGACGGCGGCGGCGGCGTGGACCGCGTC



gi|30076197|gb|CB874211.1|CB874211 HC15G11y CH Hordeum vulgare cDNA clone HC15G11 3-PRIME, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
|||||||||||| || |||||
GCCCAGCCGCGACGTACGCCG

EST sequence (targeted fragment marked with yellow):
NTGGAAGCAAATCTGCTTTATTTATTCGGCTTAAAGAGCAACTGCTGACATAGTACTGTCACAGACACACACTACACCAATCTCTCGACAAGTTTCGTACGTGGCGAACGCCCTTTATTCATCCATGGTTGCCGATGGCTTAGATTAGATGGAGATGCTGTTGGGGATGCCCTTGGCGGTAAGCCCGGCAGCGGCGCCCTTGTGGTCGGAGGTGTTGGGGTAGAGCAGCATGTAGGGAAACTTAGCCGGGCCGTTGCGGTTCTTGAGCTCCGGGTCATGGTTCATGCCCACCACCTTGCTCTCGATCTCCACCAGCCGGTCGCTGAACCGCTTGAACACCTCCAGGGCCTTTGGGTCCGAGGTCCACTCCGGCGTGTCCCGCTGCCCGAGGTACAGCTCGTCGGAGGAGTGCTTCGACAGCACCTCCAGCAGCGACACGCCGATGATGGTCTGGATCTGGCTCGTGATGGTGTGGATGAAGGCCCGCTCCGGGTCGCGCTCCAGCTCCGCGTACTCCTCCGTGCCGGGCTCCGGCATGCGGCGCCGGCTCACCGTCGGCCGGTTCGGGAGGAACCCGCGTAGGGGTACTGCCCGAAGTTGACTGCCGCATGCAGCGCCGACCCGATCCAGATGATGGTGGTGCACGCC



gi|44807142|gb|CK124140.1|CK124140 BES1824107d01 BES1824 Hordeum vulgare subsp. vulgare cDNA clone MPMGp2010D01107 5-PRIME, mRNA

CGGGTCGGCGCTCCACGCGGC
|||| ||||||. |||||||
GCCCCGCCGCGGTGTGCGCCC

EST sequence (targeted fragment marked with yellow):
CACGCATCCGCATCCGCATCGGGCTTTCACCCCCAGTGCTGCAGATCGGATCCCCATCCATGGCGGGGCGCTTCGACCTCCAGGGCAGGCACGGCAAGTCCCGCGTCCGGGTCTCCCGCGTGTGGCGCCGCCCCGCCGAGGCCGGCGGCCACCTCTTCGTCGAGTGGAGCGTCGCCGTCAGCGTCGTCTCCGACTGCCTCCCCTCCTACACCTCCGACGACAACTCCGCAATCGTCGCCACCGACTCCATCAAGAACACCGTGTATGTGAAGGCCAAGGAGTGCACGGAGGTGGTGTCCATGGAGGAATTCGCGGTCATCCTCGGCAGGCATTTCACCTCCCTGTACCCGCAGGTGTCGGAGGCTACAGTGACGATCGTGGAGCGCCCATGGGAGCGTGTGGNTGTGGACGGGAAGCCCCATTCGCATGGGTTCAAACTTGGTTCTGAAAAGCACACCACTGAGGTCACTGTGAAGAAGTCTGGAAGCCTGCTTATAAATTCTGGGATACAAGGATACTCCTTGCTAAAGACAACTCAGTCTGGATTTGAAGGATTCGTGAGGGACCGCTACACTCTTCTTCCTGAAACAAGAGAAAGAATCGTAGCAACAGAAGTAACTGCTTGGTGGAGGTATCCGTTCGAGGATATTTCCCAGCTTCCGTCAAAGCCATTTTGCTTCACACAAAGATACCAGGATGTGAAGAAAGTTCTCGCAGACACATTCTTTGGTCCTCCTGATGTTGGTGTGTATAGTCCATCAGTGCAAAATACATTATACCTCATGGCTAGGGAAGTTCTTACCAGGTTCCAGAACATAGCATCCGTTCAGCTTAAGATGCCAAAACCTCCACTTTCTTCCCGGGAAACNTAGGGGGGGAAAAAAAAATCCTGGAATTGGNNAAAGTTTGCTGAAGGATGTGTAANTTGCCNGAATTGATGAACCC



gi|217040813|gb|GH206557.1|GH206557 HV_CEa0004C19r2 Hordeum vulgare (Blumeria challenged) seedling green leaf cDNA library HV_CE

CGGGTCGGCGCTCCACGCGGC
|||||||||||||||. .||
CCCCAGCCGCGAGGTGTATCG

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTGAAGAACTTTCAGGTAAAACCCCACTGATAATCTCGCTAGTCCAACTGATAGGGAAAACGTACAGTACCGCGTAACAATGATTCATCCGAACATTACAGTTATTACGTGATTCCCTGAGTTCACTCCCCTATGGACTTATAGTGCTGATAAACACACGGTGCTATCTGTAGAAGTAATAATAATAATAATAATAATAATAATAAAGTTTGTGCCTTCCTCTACGCCAAATAGTGCTGCTGATGGATGGCGTCCAGAAATAAAATTATTGTTGTTTCCTTCAAGAATGCCATATGACTACATAAGCTATGTGGAGCGCCGACCCCGGTGCTGCTACTTGTGCGGATGCGACTCTTCGTCGGGTTCAACCTTCCGGGGCAGTCTCTTCTTCAGCGTGTACTTTGCGTACGAGTACCACACGCCGCCGAACGTGTTGATCACCAGCCCGGTGACATTGAGGGCGTGCACCTTGACGCCTCCCAACACCACGAAACCCAGGGTAGTGGACCCGACGCCCTTGAGGACTCCTACTATCGTGGTTGTCAAGGCAGAGTTTACGATTGTACACCAGAACATGGTGTAGTTGAGAACGATGCCCATCACCAGTGAAATTAGAAGAATAACACTGAATGATGCAGAAGCTGTCTTTTCTGACAGGACTG



gi|16307787|gb|BI956534.1|BI956534 HVSMEn0003P21f Hordeum vulgare rachis EST library HVcDNA0015 (normal) Hordeum vulgare subsp.

CGGGTCGGCGCTCCACGCGGC
||||.||.|||||| |.|| |
GCCCGGCTGCGAGGGGTGCGG

EST sequence (targeted fragment marked with yellow):
CCGGCGTCTTCACGGGCGTCACGGGCCCGCTCGGCGGCGTCGGGGGCGGCGTGGGGAGCGTCGGCCCGGTCGGCGGCGTCNGGGGAGCCGGCGGCATCCCTTTCAGAAGCTTCNGGGGTGGCANCNCCCCCTTCGGCGGGGTCAGGGGCGGGTACAGAGGAAGAGGCGCCTCCAGCTTCTGGCCGTGGAACGTGGCTTTCTGGCCGTAACTACAGCGTGCCTTCCCCGCGTACAGTTACCTCTGTCTCACATGGTGTGTCTTGGGCCCCGTCTAATATCTCACCTATGGGGAGCCGGTCTGTGGGGGGAGCGAGGGGCGCGGAAACAGGTGGAGTCGGAGCGCGGGCGCGCGCCGTACAAGATGGGGGCGCCGCGCAAACCATGAGCGATGGGAATGACTACTTCCGGGGGACTTCGGCGTTTGGGTTCGGGGGAGGGTGGAAGCTCGCGGCGGCGCTGCCCCGTGTAGGCAGGCGGGTAGGAATGCTCGGGAGGCCTCAAGCGGCGCGCGCGGGGGATATAGGAGACGCGCCGCGTGTCACAGACATCCGTGATCTTCTGGGCTATACAGTGCGGCCGACATTAGGTAGCGCGGACGGCGCTGAGTCTGCGGCGGGTCGGGGCTATATGGCTGGCGCCGCGCACGAACGTGCAAGGGCCGGGGCTGCGGGCTCGAGGGCGGGAGTCGATGTGGAGGCGTGGGGGTGGAGGACGCCGGCGGGGCGACGTTCTCAAGTGCTGTAGGGGCGTTAAGCGCACTGGGTGCGGCGGCTGTTGCGTGTTGCAGGTGGGAGCAGGCCGTGCGAGCGGGCGAGGGCTGGTATCGCGAGGCGGGGGCGTGTTAATGAGTGAGTGCGCGCGGGCTGATGTGGAGTACATCGCGATGGACGCAGAGATGGACAGCGCGGCGCAGGCTCCGAATTGCTACATCGCGTGCGTGCCGAGTCAACTGTTGCCCCCGCGCGGTGGTCAACGATATCTATCAAGTNGTGGTGGTGTAGTTACAGGGTTAACGGGGTGGCGGGTGGCGTCATGGCGCGTAGACGTATGGTGCGCGGATGACATCAGTCGATCTGTGAGTAGTGACGCGTCGGTAAACTCCGTGGATGAGCGCGCGTGAATGTGTAGCCGCACGTGACGGGGTGGACTGCTGACGCGATCGTGATGACGTGCGATCGGGTGCGAGCGTGTCGGCCGCCGATATGGCGGACGGNTGGGTGCGNGAAGAGGATGGTCGGGAANGAGAGGCGGGGGNGGAGNGNGGNTGAGGATNNAGGGGCGGGATGCGTGACGTGCGATGTCTTAGGTGGGTCGGTGCGTATACGCGCGTACACCACCGG



gi|30073791|gb|CB871811.1|CB871811 HC05F16y CH Hordeum vulgare cDNA clone HC05F16 3-PRIME, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
|||||||||||| || |||||
GCCCAGCCGCGACGTACGCCG

EST sequence (targeted fragment marked with yellow):
NTGGAAGCAAATCTGCTTTATTTATTCGGCTTAAAGAGCAACTGCTGACATAGTACTGTCACAGACACACACTACACCAATCTCTCGACAAGTTTCGTACGTGGCGAACGCCCTTTATTCATCCATGGTTGCCGATGGCTTAGATTAGATGGAGATGCTGTTGGGGATGCCCTTGGCGGTAAGCCCGGCAGCGGCGCCCTTGTGGTCGGAGGTGTTGGGGTAGAGCAGCATGTAGGGAAACTTAGCCGGGCCGTTGCGGTTCTTGAGCTCCGGGTCATGGTTCATGCCCACCACCTTGCTCTCGATCTCCACCAGCCGGTCGCTGAACCGCTTGAACACCTCCAGGGCCTTTGGGTCCGAGGTCCACTCCGGCGTGTCCCGCTGCCCGAGGTACAGCTCGTCGGAGGAGTGCTTCGACAGCACCTCCAGCAGCGACACGCCGATGATGGTCTGGATCTGGCTCGTGATGGTGTGGATGAAGGCCCGCTCCGGGTCGCGCTCCAGCTCCGCGTACTCCTCCGTGCCGGGCTCCGGCATGCGGCGCCGGCTCACCGTCGGCCGGTTCGGGAGGAACCCGCGTAGGGGTACTGGCCGAAGTTGACTGCCGCATGCAGCGCCGACCCGATCCAGATGATGGTGGTGCACGCCTTGGCCAGCTCCGGCACACTTTGC



gi|217052152|gb|GH217262.1|GH217262 HVSMEg0005F16f2 Hordeum vulgare pre-anthesis spike (white to yellow anther) cDNA library HVS

CGGGTCGGCGCTCCAC-GCGGC
|.|||||||||||||| ||.||
GTCCAGCCGCGAGGTGGCGTCG

EST sequence (targeted fragment marked with yellow):
GTCCGCTCCACTCGCCCACGCTCGGTGCTCTCACTTCACTGCTCACCGCTCGCCATTACTGGCCTCACCCACCCCAGATCTCATCCATGGCTCCCCAGCGCCGCCACCACGCGGCGGCCACGGCATGCCTTCTCGCCCTCCTCGCCCTGTCCGCCGCGCCGGCCTACGGCAGCCGCGCGCTGCCGGCCAGCAAGGGCGCGGCAGCCAGCAAGGCGCAAGCGGCGGCGGCCAACGCGACGGCGACGGCGACGGCGGACGAGTTCCTGGCGCCGCACAACAAGGCGCGCGCGGCGGTGGGCGTGGCCCCGCTGCGGTGGAGCGCCGACCTGACGGCGGCGGCGGCGTGGACCGCGTCGCAGCAGCATAAACAGAAGAGCTGCGCGTTCGCGGACATGGGCGCCAGCCCCTACGGCGCGAACCATGGGTGGGCGAGC



gi|166076820|gb|FD527785.1|FD527785 RUS138A06w HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59K21, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
|||||||||||| || |||||
GCCCAGCCGCGACGTACGCCG

EST sequence (targeted fragment marked with yellow):
CGGCTTAAAGAGCAACTGCTGACATAGTACTGTCACAGACACACACTACACCAATCTCTCGACAAGTTTCGTACGTGGCGAACGCCCTTTATTCATCCATGGTTGCCGATGGCTTAGATTAGATGGAGATGCTGTTGGGGATGCCCTTGGCGGTAAGCCCGGCAGCGGCGCCCTTGTGGTCGGAGGTGTTGGGGTAGAGCAGCATGTAGGGAAACTTAGCCGGGCCGTTGCGGTTCTTGAGCTCCGGGTCATGGTTCATGCCCACCACCTTGCTCTCGATCTCCACCAGCCGGTCGCTGAACCGCTTGAACACCTCCAGGGCCTTTGGGTCCGAGGTCCACTCCGGCGTGTCCCGCTGCCCGAGGTACAGCTCGTCGGAGGAGTGCTTCGACAGCACCTCCAGCAGCGACACGCCGATGATGGTCTGGATCTGGCTCGTGATGGTGTGGATGAAGGCCCGCTCCGGGTCGCGCTCCAGCTCCGCGTACTCCTCCGTGCCGGGCTCCGGCATGCGGCGCCGGCTCACCGTCGGCCGGTTCGGGAGGAACCCCGCGTAGGGGTACTGCCCGAAGTTGACTGCCGCATGCAGCGCCGACCCGATCCAGATGATGGTGGTGCACGCCTTGGCCAGCTCCGGCACACTTTGCATCTTGG



gi|13157322|gb|BG344993.1|BG344993 HVSMEg0018D11f Hordeum vulgare pre-anthesis spike EST library HVcDNA0008 (white to yellow ant

CGGGTCGGCGCTCCACGCGGC
||| ||||||.| |||||||
CCCCTGCCGCGGGCTGCGCCG

EST sequence (targeted fragment marked with yellow):
GAACTAGTCTCGAGTTTTTTTTTTTTTTTTTTTTTTTAGAAAACGATACTTGTCATTAAACCACGTCGCACCTTACACAGCGGGACGCGATTACAAAATAAACCAGAGCGCAATCGATTCCCGGCCGCTCAGTCGGCAGGCAAGAAACAAACCACTCTATGTAGATTGAGAAACAAGTAAATAGTGGAAGAGAAAACAAATATTGATGCTGATGCGGGTTCAACCTCGCATCGAGATCGATCGATGTGCGCACGTAGCTAGGTAGCAGTACGCGTCTAGCTGCACGTACGGCTCCATCCAAACCCCGGCGACAGCCGGGAGATCAGAAAAGAGCCGTGCCTGCGCACGCGCGCGCGCGGNCGNCGGNCTAGTTGCCGCCGAGACCGACGGCGTTCTTGACGGTCTCCGCGGCGCCCTGGACGGCGTCGGCGGCGCTCCGCGCGACCATNTGCGCCTGCCCGCCGACCTCCTCCATAACGCCGCCGGCGTGGTTGGCTCCCGCTCCGGCCGCGTTGGGGGCAACCCCCGCCGCGTCGGGCGCCGTCCCCCGGGGTTGTGCGCCACATGGCCGGGCGTGTCCTTGTGCGTGGCAAACGACGGCCCGGGCGGGGGGGTTAAATGCGGGGACATAGTTGAAGGGGGGGGATTAAGTGGGAGGAGGCCTCCCCAAAATGGGGGGGGGTAAAATAGCCTTATAGAGCCTGGGGCGGAAAAACAGTGGGAGGGCGGTAGCTAATAAATGGGCGTTGGGGAAAGAAAAAGACAATCTGGGGCGGGGCCCAGAATATTGTACCGGCGAGAAAGGTGGAGAAACAGCGTGGGGTGCCCACGGTATTAGTGTCACC



gi|16312250|gb|BE231043.2|BE231043 HVSMEg0015P24f Hordeum vulgare pre-anthesis spike EST library HVcDNA0008 (white to yellow ant

CGGGTCGGCGCTCCACGCGGC
|| ||||||||. | ||||||
GCGCAGCCGCGGCGCGCGCCG

EST sequence (targeted fragment marked with yellow):
ATTTCTGCTCCTTTATCTTCGCCTTCTCTCTTATTCCCCACAAACAGCCTCCGCTTCTCGAGCCGAGCAAGAAATCTCCTTCGGAGGCGATAAGAGAGAGAGGGGGAGGGACGGGCAGGGCAGGCAGAGGAAGCACGCCTGCCCAGATCATAACCAGGATTCGAATTTGCCCGCTCATCAATCATGGTTATTGATTCATTATCATCGCCTCACAGGAGGTCACAAAACACATTCTTTCTGTCCTGCCCTAAGAAGCCTCAATCATCTCGTGATGACGGTGGTAGTTGGTCTGCTCTGGTTGAGCGTCATCGCTTTCTCTTGACAACGCTAGTGGGGCTTGCCTTCCTGTGCACAATATATCTTTACTTCGCGGGGACCTTGGGCGCACCAAAAGCTTGCTCAGGGTTGACGGGAGATGAAATGACTGCGGGTCAAGAAAAATCCGCCCTGCCGCATGGGAAAGCGTAAATAGCGCTACTCCGCGGGCGGGGTAGGATGGGCGCGGGGCCCAGCGCCACGGAGCTGGGGGGGGGGACGCGGGGAGGGGGTGGGTGGGGCCGCGGCGCGCTTGTAATGGAGCATGCGGGGGAGCGCGCGCGCGCGGAAGGCGGAGGNNCGCGCGGGCCGCAAGAGAGGACAGCCGGGCGGAGCGCGGTCCCCGGTCGGGCCGGGGAAGGGGCGGGGACGCGGCGGGCGCCGCGCGCGGCGCCGACGCGGGCGCGGGGGCGGGGGCGGCACGCGAGGTACCGTCGCGGCGACGCGGCGCCCCGCGGCGCGCCGGCGCCCGGCGCGGGGTCGCGCGGGGCGACGCCTCGTGGCGGCGCGAGGACGGGGGAGGCGGTGCGGGGGCGGGGCGGGCGCGCGGCGTANCTGCGCGGCGTGACGCGCGCGCGGCGGCGGANCGG



gi|166073231|gb|FD524800.1|FD524800 RUS115A08r HY Hordeum vulgare subsp. vulgare cDNA clone HY04O12, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
|||||||||||| ||. |||
GCCCAGCCGCGACCTGTACCG

EST sequence (targeted fragment marked with yellow):
CTAGTGGATCCAAAGCCGCCACCTCGCCGCTCACCAACGCGCTCCCCGCCGGCAACATCTGCCCCACCGGCCGCCTCGCCAAGCCGCTCCCCTCCTTCTCCGCGGCCTCCACCCCGCCGCCCCCGGCCCCTCCCCGCGCCATCCGCCACGACGTGCTCGGCTCCGGCACCGGCAACTACGGCCACGGCAGCATCATGCGCTCGCGCAGCGGCGGCGTGGCCCCCGAGGAGGACGCCGCCGTCCGGCGGGCCATGTCCAGCGCCGACCCGGAGGAGCTCAAGCGGGCCGGGAACGAGCAGTACAAGAAGGGCTACTTCGAGGAGGCGCTCAGGCTCTACGACCGAGCGCTCGCCCTCTGCCCGGACAATGCCGCGTGCCGGGGCAACCGGGCCGCCGCGCTCACCGGCCTCCGCCGCTTCGGCGACGCCATCAAGGAGTGCGAGGAGGCCGTGCGGATCGATCCGTCCTACGGACGCGCGCACCAACGGCTCGCGTCGCTCCACATAAGGTTGGGACACCTTGAGGATGCTCAGAAGCACCTTTCCCTGGCAACCCCGCAGCCTGACCTCCTAGAGCTGCACAAACTACAAACAGTGGAGAAGCACCTGGGCAGATGCCTTGACT



gi|21157892|gb|BJ479414.1|BJ479414 BJ479414 K. Sato unpublished cDNA library, strain H602 adult, heading stage top three leaves

CGGGTCGGCGCTCCACGCGGC
||||||| ||||| ||||||
GCCCAGCAGCGAGTAGCGCCG

EST sequence (targeted fragment marked with yellow):
GNAGGATTTCGCCGGGCGTCCGTCCGAGCTCGGANAAGAAGGCGAGCGCGATGGCAGCGAACGTGGCGCTGCTCTCCGCCCCCTTGCCCTCCACCTCCTCCGCCTCCCCCATCCTCCGTCGCCGCCCCCTGCCTCGCCGCTCCGCCCGCCCCCGTCGAGCAGCCTCCAGGCTATCCGTCGTGGCCGCGATGAGCGACGACCCGATCAAAGAGTGGATCCTCACCGAGGGGAAGGCCACACAGATTACAGGGACCAGCTCCATCGGGGGTGGCTGCATCAACGCCGCCCAGCGCTACGACACCGATGCCGGCTCCTTTTTTGTGAAGACCAACAGGCGCATCGGTCCAGCTATGTTTGAAGGGGAGGCTCTGGGCTTGAAGGCAATGTATGATACCAAGTCAATTCGTGTTCCTTTGCCGTACAAGGTTGGTTCGTTACCAACTGGCGGTTCTTTTATCATCATGGAGTTTATTCAGTTTGGTCGTTCTAGGGGTGACCAGGTAACCTTGTCCCGAAACCCCTTTCGTTCAAAGAAAAAGTCAGCTCTAGGGAGGAAACTCGCTGAAATGCATAAAGCTGCAAAATCTGATA



gi|217062293|gb|GH227289.1|GH227289 HVSMEn0003P21f2 Hordeum vulgare rachis cDNA library HVSMEn (HVcDNA0015)2 Hordeum vulgare sub

CGGGTCGGCGCTCCACGCGGC
||||.||.|||||| |.|| |
GCCCGGCTGCGAGGGGTGCGG

EST sequence (targeted fragment marked with yellow):
CCGGCGTCTTCACGGGCGTCACGGGCCCGCTCGGCGGCGTCGGGGGCGGCGTGGGGAGCGTCGGCCCGGTCGGCGGCGTCGGGGGAGCCGGCGGCATCCCTTTCGGAGGCTTCGGGGGTGGCAGCGCCCCGTTCGGCGGGGTCGGGGGCGGGTACGGCGGCGGAGGCGCCGGCGGCGCCACGCCTTGAGCATGCCGCGGTGGCCGAGCTAGCTAGGGCGCTCCGGGACATGCATACATGTACGTGAGAAGATCTGTTGTTGGTGCATGGTGGATCGCGCTAGCTTTGACTATGTATTTCCTTGAACCTAGTGTTGTGTCATCTCACGGCGCAGAGTATATGCGCAGAAGTACTTGGTATGTGG



gi|30083841|gb|CB881848.1|CB881848 HM11B02w HM Hordeum vulgare subsp. vulgare cDNA clone HM11B02 3-PRIME, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
|||||||||||||||||||||
GCCCAGCCGCGAGGTGCGCCG

EST sequence (targeted fragment marked with yellow):
GGCACGCACCCTTATTTATTGCCTTAATTATTCATGCACCGGCACAACTCTCTGTCACACACACACACACAAGCTAGCNTCCGGTGTAAGCAAGCAAGGATAGCACAAAGGCATGAGGTCTCCACGACGGACGTGCTCAGATCAGATGGATATGCTGTTCGGGATGCCCCTGGCGGTGAGCCCCTCGGCCTGTCCCGTGTGGTCGGAGGTGTTTGGGTAGAGCAGCATGTATGGGAACTTGGCCGGCCCGGTGCGGTTCTTTAGCTGCGGGTCCGAGTTCATGGCCACCACCTGCTTCTCGATGCCCTCCAGCTTCGCCCCGAACCGCTTGAACGCCTCCAGCGCCTTGGCGTCCGACGTCCACGCCGGCGTGTCGTGCTGGCCCAGGTAGACCTCGTCGGAGGAGTGCTTGGAGAGGATCTCCATGAGCGAGATGCCCACCAGGGCATGGAACTGGCTGGTGATGGTGCGGATGAAGGCCTTCTCCGGGTCTCGCTCCAGCTCCGCGTACTCCTCGCTCCCCTGCACCGGCATCGGCCTCCGGCTCGCCGACGGCTTGTTGGGGTGGTAGCCCGAGTAGGGGTACTGCCCGAAGTTGACGGCCGCGTGGAGCGCCGACCCGGT



gi|24299284|gb|CA021910.1|CA021910 HZ41I08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ41I08 5-PRIME, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
|||| ||||||. |||||||
GCCCCGCCGCGGTGTGCGCCC

EST sequence (targeted fragment marked with yellow):
GGGACGAGGGCGACACACGCATCCGCATCCGCATCGGGCTTTCACCCCCAGTGCTGCAGATCGGATCCCCATCCATGGCGGGGCGCTTCGACCTCCAGGGCAGGCACTGGAAGTCCCGCGTCCGGGTCTCCCGCGTGTGGCGCCGCCCCGCCGAGGCCGGCGGCCACCTCTTCGTCGAGTGGAGCGTCGCCGTCAGCGTCGTCTCCGACTGCCTCCCCTACTACACCTCCGACGACAACTCCGCAATCGTCGCCACCGACTCCATCAAGAAC



gi|217053873|gb|GH220602.1|GH220602 HVSMEi0012G20f2 Hordeum vulgare 20 DAP spike cDNA library HVSMEi (HVcDNA0010)2 Hordeum vulga

CGGGTCGGCGCTCCACGCGGC
||.|.||||||. | ||||||
GCTCGGCCGCGGTGCGCGCCG

EST sequence (targeted fragment marked with yellow):
CGACCACCCCTCCTCCCCCGTCCGGCAAACCCTAGCCCGTCGCCGCCGCCAATGGAGGAGGAGGAGGAGGAGGAGGTAGAGATCCGCGACTGGGCGGGGATGCCGTCGGACGCGCTGTTCGCGGTGTTCGGGAAGCTGGACGTGGCCGACATCCTGGCGGGGGCCGGCCGGGCGTGCCGCGCGTGGCGCCGGCTCGCGGACGGCGACGCCGCGCTGTGGCGCCGCCTCGACATGACCCACCACGGCGACATCCTCGAGACCGATGAGGCCGATGCCATGGCCCGCGCCGCCGTCGACCGCGCCGCCGGCACCCTCCATTCCTTCTGCGCCGACACCTTCGTCACCGACGCCCTCCT



gi|30073287|gb|CB871307.1|CB871307 HC03D19y CH Hordeum vulgare cDNA clone HC03D19 3-PRIME, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
|||||||||||| ||||| ||
GCCCAGCCGCGACGTGCGGCG

EST sequence (targeted fragment marked with yellow):
GATACAAAACTCACTTTATTATTATATAAATTGGAAAGGAAATTTAAAAATGGAAAAATACATTAACCCTTGGCCAACCACTCAACATTTTCAAATGCTTGTGTTGTGGGCTTGTGGCCGTCCTCCACACATCTCATCATATAGAGATGCTGTTGGGGATGCCCCTAGCAGTGAGCCCCTCAGCCTTGCCCGTGTGGTCGGAGGTGTTGGGGTAGAGCAGCATGTATGGAAAGTGGACCGGTCCATTGCGGTTCTTGAGCTGGGGGTTTCCGTTCAAGGCGACCACCTCGCTCTCGATGCCCTCCAGCCTCGTGCCGAACCGCTTGAACGCCTCCAGCGCCTTGGCATCCGACGTCCACTCCGGCGTGTCACGCTGCCCGAGGTAAATCTCGTCGGAGGAGTGCTTGGATAGGATCTCCAGCAGAGAAATGCCAATGATGGCCTGCAGCTGGTTGGTGATGGTGCGGATGAACACCTTTTCTGGATCGCGGGCCAGCAATGCGTACTCCTCGGTGCCTGGCTCCGGCATCGGACAACGGCTCGCTGATGGCTTGTTGGGGTGGTAACGGCGTACGGGTACTGCCCGAAGTTGACGGCGGCGTGCAGCGCCGACCCGGTCCAGATGATGGTG



gi|16292407|gb|BI950369.1|BI950369 HVSMEl0020G16f Hordeum vulgare spike EST library HVcDNA0012 (Fusarium infected) Hordeum vulga

CGGGTCGGCGCTCCACGCGGC
||||.||||||| ||||||
GCCCGGCCGCGACTCGCGCCG

EST sequence (targeted fragment marked with yellow):
AAGAAAAGCCATCAATTAGAGTGATCATTCTGTTTGCCTATTTTGCTCTATCCGGGGGAGGTACCGAAAGAAAATGTATAAAAGGAGGGAGCAATACTTCTAGGGTCCTCATGAATTGTCAAGTTAACAAGACTATTATGGTTGAAGGTGGAGATGTATATGACTGCGTTGGTGTGAATCTGCAACCCGCCTTTCACCACCCATTGCTGAGAGATCACACAATCCAGATGGAACCCAGTTCTCTCCCGTTTAATGTGCACACGGAATCTCCATCATCGATGCATACTATCCCACAAGCACAGTTGTCTATCATTGACTGCCCTACAGGAACGATTCAATCCTGGGGTACAATAGAGGGGACCCGTGGCACATATAGTACTGGACAAGGGGTTGCCAAAGGTGAACAACAAGAGGCCGCAGGAATCAATTATTTTGATGAACCTATCCGGACCGCGACCAAAAATAATTGTTTATGACCCAAAGGTGAAGACCTGGAAGTNGGGATCTTAGGGCATCATCTATACAAATTGGGGGAAGGGGCTGAAGAACCAATGCAGAATNAAAAAGGCGTCCGTCCTTGGGATACTCCACCTCCCCCTGGGGCCGAGCTTGCGCGGGCCCCAATCTCACGGGGCTAAGGGGTTCCGAAAACGGCAAATGCTTACACCATGACGGCGGGGGGCGGGGCAAAAAAGCCTATCCCGAGGACCCCGCGACACCAACCTGGGCGGCCGCGGGCCGGCGGACGCGAAGAAAAACAAAGGGGGCAGCGCAGGGGGGCCGGGGACGCGCACTTATCCCCGGGCCGTGGCAGGAAGCCCACCAACGTGGGTCGGCGACAACACGCGCCGCGCTCAGCGCCGGCCCGTCGCGCATCCGCCCACTAAACGGCGGGCGCGGCCGCGCGCCCGACCCGCCCCA



gi|51519677|gb|CV056538.1|CV056538 BNEL18H5 Barley EST endosperm library Hordeum vulgare subsp. vulgare cDNA clone BNEL18H5 5' s

CGGGTCGGCGCTCCACGCGGC
.|||||||||||| |||||
CTCCAGCCGCGAGGGGCGCCA

EST sequence (targeted fragment marked with yellow):
CTCGAGGCGACTCCCATCTGTCACCCTAATCTCTCGCCCCTCTCTCGCCGCCCCGCGAGTCCCCACGCCGCTGCCGCCCCGTCGGTTGTCGAACGCCGCCCCGTCGCTGTCGCCGTCGCCGTCGCCGTCGCGAACCTCCGCTCCGGTTCCGTCTCCGCCTCCACGCCTGCTCCGTCCCCGACGTCACACCGTATCCTGCCTCTCCCTCTTCTACCTCAACCGCGGGGAGCGCCGACCTCGCCGACCTCCACTGCCCCTCTGCTACATCGCCCGCCTATAGCGAGAAGGACCTGTTCGTTGGGTACTGCCATCGATGGAGAGCAGTGAACCACTCCAGGATATAATGTGTGAGTTCCGTGCTGGTAAGATGTCTCTTGAGGGAACACGGGTGGTCCCAGACACACGCAAGGGGCTTGTTCGTATTGGAAAGGGTGAAGAAGGCTTGGTTCATTTTCAGTGGCTTGACCGTGGACAAAACATAGTTGAAGATGATCAAATTGTCTTCCCTGATGAGGCTGTCTTTGAGAAGGTAGCAGAATCTTCTGGAAGGGTGTACATCTTGAAGTTCAGGCATGATAGCAGGAAATTCTTCCT



gi|16334204|gb|BF263010.3|BF263010 HV_CEa0005I24f Hordeum vulgare seedling green leaf EST library HVcDNA0004 (Blumeria challenge

CGGGTCGGCGCTCCACGCGGC
|||||||.||| ||||.|| |
GCCCAGCTGCGCGGTGTGCGG

EST sequence (targeted fragment marked with yellow):
CACGCGACGCCGGGGGACGTGCGCCTCGTGCGCGGCGCGCGCGGCGACCGGGTGTGCGCGCTTTCCTGCTCCGGCCTCGACGTCGCCGGCGACCCTCTTGGGACGTCCGCCTGCGTCGCCATGAAGGCCCCCGGCGTGTGGCGCGTCGACCCGGTGCTGGCCGGGGGTGGGCGACCGCGTGGGCGCCCTCGGCGGCGCGTGCCAGCTTGCGCTCGCCGAGGGAGAGGACGCCGCCGCGGGGGAGGTCCACGAAGGCGGGCACTGGACGGCCTGCGATCCGATGCCGGCGGAGCTCCCCGAGTCCGCCGCGGCAACGGGGCTTTTCGGGGGCCCCACCGATCANAGAGTGTCCCTCNCCGACAGAAGCACGGGCTGGCCAACTGGGGCGGTCCCGTGGAGCGGCGGGGGGGGCCCGGGCGAAGCGTGAGGCCGGGCGGGCGGGGGTGAAGGGGGGGGGTGAGCCCGGGCCGGAGCGGGGGCGAGCGGAGTGGAGGGGTGACGGGCGGGGCCCGCCGGGGCAGAGGGACCAAGGAGAAGGTGAGCGGTCCAAGCAGGGAAAGAGGGGCAGCGAAACACATAGGGACGTTGGGGTCGCCCGGGGGCGGCACCCGACAACCAGACGCGCCGCCGGAGGGGTGGGCCGGAGGCGCGGCCCGCCCCGCCGGGGGCGAAGGGGGCGGGAAGGGGGAAGCGCCTCCGCCGGGTCGGGTAGGGCGCGCGACGAACCGCCCCGACAAGGGGCGCGGGGCAAAAACGAGGCGGGGCACGAGCCCCCGGGCGCCCCACACCAAGGAGAGCAAGAGGCGAGGGAGGGGANNGGTGAGGGCGGGTGGGTAG



gi|21277212|gb|BQ469430.1|BQ469430 HM04K23r HM Hordeum vulgare subsp. vulgare cDNA clone HM04K23 5-PRIME, mRNA sequence

CGGGTCGGCGCTCCACGCGGC
||||.||.|||||| |.|| |
GCCCGGCTGCGAGGGGTGCGG

EST sequence (targeted fragment marked with yellow):
CGGCACGAGGGGCTTCCCCGCTGTGGGCGGCGGCTACGGCGCTGGCTTCGGCAACAACGGAGCTGGCGTATTCACCGGCGTCACGGGCCCGCTCGGCGGCGTCGGGGGCGGCGTGGGGAGCGTCGGCCCGGCCGGCGGCGTCGGGGGAGCCGGCGGCATCCCTTTCGGAGGCTGGGGGGGTGGCAGCGCCCCGTTCGGCGGCGTCGGAGGCGGCTACGGCGGCGGAGGCGCCGGCGGCGCCACGCCTTGAGCAGGC