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mature miRNA: TCGCTTGGTGCAGATCGGGAC
species: Hordeum vulgare
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|18222117|gb|AV926320.1|AV926320 AV926320 K. Sato unpublished cDNA library, cv. Haruna Nijo second leaf stage seedling leaves

TCGCTTGGTGCAGATCGGGAC
||||.||||| |||||.|||
CGCGAGCCACGCCTAGCTCTG

EST sequence (targeted fragment marked with yellow):
GAGAGAGAGAACTAGTCTCGATCCGCACCGAGCGCGCACCGGTGGAGATCGTGGTGGGAGGATGTCGTCGGACTACTCGTCGTCGTGGGCGCGCGCGCTCGTGCATATCTCGCCCTACACCTTCTCCGCCATCGGCATCGCCGTCTCCATCGGCGTCTCTGTCCTCGGCGCAGCATGGGGGATCTTCATCACGGGGAGCAGCCTCATCGGGGCCGCCATCAAGGCGCCCAGGATCACCTCCAAGAACCTCATCAGTGTCATCTTCTGTGAGGCCGTTGCAATTTATGGTGTAATCGTGGCAATCATCCTCCAAACAAAGCTTGAAAGTGTGCCAACATCCCGAATGCATGATCCAGAGTCTATGCGAGCTGGTTATGCAATCTTTGCCTCAGGTCTTATTGTCGGCTTTGCTAATCTTGTTTGCGGGGTATGCGTGGGAATAATCGGAAGCAGCTGTGCACTGTCTGATGCCCAGAATTCAACACTCTTTGTAAAGATCCTGGTGATTGAGATCTTTGGCAGTGCTTTGGGCCTCTTTGGAGTGATCGTGGGTATAATCATGTCAGCTCAAGCAACATGGCCAACGAAAGTCTAATACCATCCATCTGGGAATCTCTGTAA



gi|21149273|gb|BJ470770.1|BJ470770 BJ470770 K. Sato unpublished cDNA library, cv. Haruna Nijo adult, heading stage top three lea

TCGCTTGGTGCAGATCGGGAC
||||.||||| ||||||| |
CGCGAGCCACGNCTAGCCCCG

EST sequence (targeted fragment marked with yellow):
ATTCGGCACGAGGCCCCGATCNGCACCGAGCGCGCACCGGTGGAGATCGTGGTGGGAGGATGTCGTCGGACTACTCGTCGTCGTGGGCGCGCGCGCTCGTGCATATCTCGCCCTACACCTTCTCCGCCATCGGCATCGCCGTCTCCATCGGCGTCTCTGTCCTCGGCGCAGCATGGGGGATCTTCATCACGGGGAGCAGCCTCATCGGGGCCGCCATCAAGGCGCCCAGGATCACCTCCAAGAACCTCATCAGTGTCATCTTCTGTGAGGCCGTTGCAATTTATGGTGTAATCGTGGCAATCATCCTCCAAACAAAGCTTGAAAGTGTGCCAACATCCCGAATGCATGATCCAGAGTCTATGCGAGCTGGTTATGCAATCTTTGCCTCAGGTCTTATTGTCGGCTTTGCTAATCTTGTTTGCGGGGTATGCGTGGGAATAATCGGAAGCAGCTGTGCACTGTCTGATGCCCAGAATTCAACACTCTTTGTAAAGATCCTGGTGATTGAGATCTTTGGCAGTGCTTTGGGCCTCTTTGGAGTGATCGTGGGTATAATCATGTCAGCTCAAGCAACATGGCCAACGAAAGTC



gi|18222127|gb|AV926330.1|AV926330 AV926330 K. Sato unpublished cDNA library, cv. Haruna Nijo second leaf stage seedling leaves

TCGCTTGGTGCAGATCGGGAC
||||.||||| |||||.|||
CGCGAGCCACGCCTAGCTCTG

EST sequence (targeted fragment marked with yellow):
AATTCGGCACGAGGAGAGAGAGAACTAGTCTCGATCCGCACCGAGCGCGCACCGGTGGAGATCGTGGTGGGAGGATGTCGTCGGACTACTCGTCGTCGTGGGCGCGCGCGCTCGTGCATATCTCGCCCTACACCTTCTCCGCCATCGGCATCGCCGTCTCCATCGGCGTCTCTGTCCTCGGCGCAGCATGGGGGATCTTCATCACGGGGAGCAGCCTCATCGGGGCCGCCATCAAGGCGCCCAGGATCACCTCCAAGAACCTCATCAGTGTCATCTTCTGTGAGGCCGTTGCAATTTATGGTGTAATCGTGGCAATCATCCTCCAAACAAAGCTTGAAAGTGTGCCAACATCCCGAATGCATGATCCAGAGTCTATGCGAGCTGGTTATGCAATCTTTGCCTCAGGTCTTATTGTCGGCTTTGCTAATCTTGTTTGCGGGGTATGCGTGGGAATAATCGGAAGCAGCTGTGCACTGTCTGATGCCCAGAATTCAACACTCTTTGTAAAGATCCTGGTGATTGAGATCTTTGGCAGTGCTTTGGGCCTCTT



gi|24328355|gb|CA031940.1|CA031940 HX11I23r HX Hordeum vulgare subsp. vulgare cDNA clone HX11I23 5-PRIME, mRNA sequence

TCGCTTGGTGCAGATCGGGAC
.|||.|||||||||||| . |
GGCGGACCACGTCTAGCGTCG

EST sequence (targeted fragment marked with yellow):
GGAATTCGGCACGAGGGACGGGCCCGTGCTGCTTCTCTCCCGACGCCCGCTTACTCGCCGTCGCCGTCGACTACCGCCTCGTGATCCGCGACGTCGTCACCCTCAAGGTGGTGCAGTTGTTTTCGTGTGTGGACAAGATAAACTTTTTGGAGTGGGCACCGGATTCAGAATACATTCTATGTGGACTTTACAAGCGGCCAATGGTGCAGGCTTGGTCACTTAGCCAGCCTGACTGGACCTGTAAGATTGATGAAGGATCTGCAGGGATTGCTTATGCTCGCTGGAGTCCTGACAGTCGGCATATACTCACTACATCAGAGTTCCAACTTCGTTTGACTGTATGGTCTCTTGTAAATACTGCCTGTGTTCATGTACAGTGGCCAAAGCATGCAGCAAGAGGTGTTTCTTTCACTAAGGATGGCAAGTTTGCTGCGATCTGCACCAGGCGGGACTGCAAAGATTACGTAAATTTGCTGTCATGTCATTCCTGGGAGATAATGGGTGTATTCGCTGTTGACACACTAGACTTAGCAGGCGTGGAGTGGTCGCCTGATGACAGTGCTATTGTGGCCTGGGATTCACTTCTTGAATACAAGGTTCTAATATATTCACCAGATGGGCGATGCTTGTTCAAATATTCGGCATATGAAAGTGGGTT