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mature miRNA: ATATAGCATCATGAGCAATCA
species: Lotus japonicus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|223451809|gb|GO041502.1|GO041502 LJNBF03TO JCVI-LJ3 Lotus japonicus cDNA 3', mRNA sequence 

ATATAGCATCATGAGCAATCA
|.||||||||||.  |||.||
TGTATCGTAGTATCAGTTGGT

EST sequence (targeted fragment marked with yellow):
GGAAAGCAAACCTTGCGATATTGAGATGGATGGTTGATAAGGAATACAACACTTCGGAATTCAAATGCCAGTCTCTATGCCCTGTGTCTGTGTGAAATGTTGGAAATTTTCACCTTAATTTTTGGTTGACTATGATGCTATGTCCCTGAAGGCTTCTGAAAGTCATGTCAAGGGTTCTCAAAATTGCTGGCTACCAGTCTACCTCAAGTGTTGAGATACTCAGACAGACGGATTAGTCGGAGTAGGGGGTGAGCATATGCGTGTTGTCCATCTTGTGCCTGGTTTATTTGATGCTGGTAGCTTGATAGAGTTTACAACTGCCAGCAATAATGTATTGCAGAGTGTAGCCATCTTGTGCCTGGGTTATTTGATGCTGGTAGCTTGATAGAGTTTACAATTGCCAGCAATAATGTATTGCAGAGTGTAGCATAATGATTGAATTCATCTCA


gi|45628438|gb|BP071722.1|BP071722 BP071722 Lotus corniculatus var. japonicus nodulating root Lotus japonicus cDNA clone GNf059a 

ATATAGCATCATGAGCAATCA
 ||||.|||||||||||||||
CATATTGTAGTACTCGTTAGT

EST sequence (targeted fragment marked with yellow):
GTTGGGAGTGACAATGGAGCAGACTATTGGGCTCCTGCCTTTGGAGGGGGCTTTCAGTAAATCAACTTAACGATTGGGGGCAGCAGCATATACATACAAATATGGTGGCATATTGTGCGTGCAGAAGGTAAATTCAAGGAAATGATATAACATCATGAGCAGTCCATCTGCCTAAAAAAATTAAGGCTGATTGCTCATGATGTTATACTTCATTATTCCTGAATAAATTGTATGCAAGCCAGCCATGATGATAAGTATAAGATGGTGGTGTCAATATTGCGCTATATTTCAATGTATATGCAATATCTGTATCATTAATTATCATTTGAATGCTACATTTTTAGCAGCATTAACCAATATTATTTGTATTATTGCTAGTACTTTTTTT


gi|45637515|gb|BP080854.1|BP080854 BP080854 Lotus japonicus roots Lotus japonicus cDNA clone MR063f09_f 3', mRNA sequence 

ATATAGCATCATGAGCAATCA
|||||||||||||||||||||
TATATCGTAGTACTCGTTAGT

EST sequence (targeted fragment marked with yellow):
AGGGTTGTGTCTGGTTCCAGTTTCATGCACACTCCAAGTTGGAAGTGACAATGGAGCTGATTATTGGGCTCCTGCTTTTGGAGGGGAATTTCGTTAATTAGATCTGGTTTTAGCCTATTAACTGATGCAGCATATATACATATATCTATCTAGCTATATATATGTGGGCAGCATTTAGAGAGAAAATTGATGGAAATTGTATAACATCATGAGCAGTCAATCTGCAACAAGATCCAAGGCTGATTGCTCATGATGCTATATATGCACACCATTCCTCATGAATAATTTGTGTGTTGCTCGCTGCAGTCAACTATACATTTGCAAACCCTTGTTAATTAGCAGTCTTCAATTTATGCAAATCAAGCTTTTACTT