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mature miRNA: TGACAGAAGAGAGTGAGCACA
species: Lotus japonicus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|7746737|gb|AV417559.1|AV417559 AV417559 Lotus japonicus young plants (two-week old) Lotus japonicus cDNA clone MWM145h11_r 5'

TGACAGAAGAGAGTGAGCACA
||||||||||||| |||||||
ACTGTCTTCTCTCTCTCGTGT

EST sequence (targeted fragment marked with yellow):
AGGGACATCCTATGTCAAGATCTCTATTTCCTTCACACAACCAAAAACAATTTCCCTTTCTTCATCATGAAAATGGTGCCGCGTCCACTACCGAAAGCATCTTCTGTGATAGCAATGGCCAATATCCACCTTCCCTCGGGGCTGCCCAAAATTCTGGTTTACGACCACTATTCCACGACACCTCCTTAGGAAGTGAGGACTTCAATGTTTTCTACACACCATCAACTGTTCAGGGATTATCGGGAATTTCAGACTCTTGTGCTCTCTCTCTTCTGTCATCACAATCCCAGAACTCGCCAAGCCAATCGTCAGGAATTCCTTTGGCTCACCCCTTTGTTATTCCCAGCAGCCATAGCCATCACTACAGCATGAGTCAGGTCTCGGAAAAGATAGGAATAAGCTCACACACTTCTTCAAGCC



gi|45612761|gb|BP056010.1|BP056010 BP056010 Lotus corniculatus var. japonicus pods (less than 20 mm in length) Lotus japonicus c

TGACAGAAGAGAGTGAGCACA
||||||||||||| |||||||
ACTGTCTTCTCTCTCTCGTGT

EST sequence (targeted fragment marked with yellow):
TTTCAGCTGCTTCTGTGAGCCCCTTGCTTCTCAAGGACCAGTTCCGTTCAGGACTCCCCCTGTGCTCTCTCTCTTCTGTCATCATCATATTCATCACCGACTCACGCCCCTGGGAATGGTTTGGATCCAATGGTGCAGCCTCACGCCATGTCAATGTCCCTTATGGAGCCCTTAGGCCTGAGCCTGCATGATAATAACAGCCGCTTAGAGTCCCTGGACCCCGTCTTGGTTCATAATGGGAGTGACCCTTGTTCCTCCATGTATAACACTGGGTTCTGATGGATCCCCGAGCCATGAAGCCCCTCCACTATTTCCTTTTCCATGGGAATAATTTTTTGGGAATTGGATGGAATGGGGTAGAATGAATAAAAATACACTTCCCTTATTTGGATATTTTGCCATTAAATTGAATAAAGCTTTCACTCCCTTATTTGGCTTGGCTAATTAATGGAATGATTATTTTTTAT



gi|45608292|gb|BP051612.1|BP051612 BP051612 Lotus corniculatus var. japonicus pods (less than 20 mm in length) Lotus japonicus c

TGACAGAAGAGAGTGAGCACA
||||||||||||| |||||||
ACTGTCTTCTCTCTCTCGTGT

EST sequence (targeted fragment marked with yellow):
ACCCACATCTTCCAGCTGCTTCCATAGGCCATAAGTTTGGCAGGCCTAGCCCTTTTGCAGAAAGTGATGGGCTGAGATGCAAAAACATGTTCTGTGATAGCCTAACAAGTTCAGTCCAAGAGTCCTCTTGTGCTCTCTCTCTTCTGTCATCACCACAGGCACACAACACTGCAGGAAATGGGTTTAACCAAATGGTGAACCCTCACTCATCCCTTATGCAGCCCTTGGGCCTGAGCCTGCATGATAACAGCATGGGGCCCGTGTACTCAGGTTGTGATGACCATTGTTCATCAATGTATAACGTGGGTTCTAGTGGGTCCCAGGGGAATGAAGCCCCTTCAATGTTCCCCTTTCAATGGGAATAGAGGGTTTTAGGTTTTATCACAAGTACTTTTTTAATTATCAACTTATCTCTGGACAAGGCCATGAGGACTATCACCTTAGCCAAACACTTGGTGTGAATTTTTTAAGTGGTGGGGTTGCTACCGTGCATACATCCACTATTGCAGT



gi|293548593|gb|FS354274.1|FS354274 FS354274 LjFL3 Lotus japonicus cDNA clone LjFL3-029-CC02.r0 3', mRNA sequence

TGACAGAAGAGAGTGAGCACA
|||||.||||| |||.||||
ACTGTTTTCTCGAACTTGTGT

EST sequence (targeted fragment marked with yellow):
CAAGTTCATCTGTAAGGATTTTTGGTCTGAGGTCTTTAAGAAGCAAGTAGACAACTTGAAGACAAATCATAGGGGTACCTTTGTATTACAAGATAATAAATTTCCCTGGCTTTCACGGATGTCAGTTGATCCATCTGCTGAGAATGTTAGTTCCGTTGAGGATAATACTTCACCTACACCTGAAAGCAAGGCAGCACAAGCAATGAGCATGCATCTTTATTTCCCATGTGGGATCATTAGAGGAGCTCTTTCCAACTTGGGAATTCCCTGTGCAGTTTCTGCTGATATATCAAACCTTCCAGCATGTTCATTTGTGGTGTGCATAAAAGCTTGATGAGTTCATTTGCATTACTGGCGGGAGGGAAGGCCTTCACATTGTTTGAAATAATGCTTTAATGTGTTCAAGCTCTTTTGTCATCAATTTACTGGACCGGGGAATATTTCCTTACTATATTTAAGCTATCAAATGTCTTGGTTGTATAGGAAGGGCTCCTGGGGAAGTGTTTTGATAAGTGAGGACTTGGTTCCTTCGGATACAGATTCTAGGGAGATTAAGAGAGTATGGTGCGTAGTTCCCAAACTGTGGTGTAGGGTGGAAAGATTTTTGGGAATTTCAGTCCTTGTAATGTTATCACTCTTGTATTGAATGAATGCTAACTTTATATTTTAAAACTTTTGTTCCTTATTTGATTACCAAAAAAAAAAAAAAAA



gi|45608109|gb|BP051429.1|BP051429 BP051429 Lotus corniculatus var. japonicus pods (less than 20 mm in length) Lotus japonicus c

TGACAGAAGAGAGTGAGCACA
||||||||||||| |||||||
ACTGTCTTCTCTCTCTCGTGT

EST sequence (targeted fragment marked with yellow):
ACCACAATTGATAACCAACTTTCAGCTGCTTCTGTGAGCCACTTGCTTCTCAGGACAAGTTCAGTTCAGGACTCCCCTTGTGCTCTCTCTCTTCTGTCATCATCATATTCATCACAGACTCACGCACCTGGAAATGGTTTGGATCAAATGGTGCAGCCTCACGCAATGTCAATGTCCCTTATGGAGCCATTAGGCCTGAGCCTGCATGATAATAACAGCAGCTTAGAGTCCATGGACCCAGTCTTGGTTCATAGTGGGAGTGACCATTGTTCCTCAATGTATAACATGGGTTCTGATGGATCCCAGAGCAATGAAGCCCCTCAACTATTTCCTTTTCAATGGGAATAATTTTTTGGGAATTGGATGGAATGGGGTAGAATGAATAAAAATACACTTCCATTATTTGGATATTTTGCAATTAAATTGAATAAAGCTTTCACTCCATTATTTGGCTTGGCTAATTAATGGAATG



gi|45607810|gb|BP051130.1|BP051130 BP051130 Lotus corniculatus var. japonicus pods (less than 20 mm in length) Lotus japonicus c

TGACAGAAGAGAGTGAGCACA
||||||||||||| |||||||
ACTGTCTTCTCTCTCTCGTGT

EST sequence (targeted fragment marked with yellow):
GTTTGGCAGGCCTAGCCCTTTTGCAGAAAGTGATGGGCTGAGATGCCAAAACATGTTCTGTGATAGCCTAACAAGTTCAGTCCCAGAGTCCTCTTGTGCTCTCTCTCTTCTGTCATCACCACAGGCACACAACACTGCAGGAAATGGGTTTAACCCAATGGTGAACCCTCACTCATCCCTTATGCAGCCCTTGGGCCTGAGCCTGCATGATAACAGCATGGGGCCCGTGTACTCAGGTTGTGATGACCCTTGTTCATCAATGTATAACGTGGGTTCTAGTGGGTCCCAGGGGAATGAAGCCCCTTCAATGTTCCCCTTTCAATGGGAATAGAGGGTTTTAGGTTTTATCACCAGTACTTTTTTAATTATCAACTTATCTCTGGACCAGGCCATGAGGACTATCACTTAGCCAAACCTTGGTGTGAATTTTTTAAGTGGTGGGGTTGCTACCGTGCATA



gi|45609330|gb|BP052650.1|BP052650 BP052650 Lotus corniculatus var. japonicus pods (less than 20 mm in length) Lotus japonicus c

TGACAGAAGAGAGTGAGCACA
||||||||||||| |||||||
ACTGTCTTCTCTCTCTCGTGT

EST sequence (targeted fragment marked with yellow):
GACTCCCCTTGTGCTCTCTCTCTTCTGTCATCATCATATTCATCCCCGACTCACGCCCCTGGAAATGGTTTGGATCCAATGGTGCCGCCTCACGCCATGTCCATGTCCCTTATGGAGCCCTTAGGCCTGAGCCTGCATGATAATAACAGCCGCTTAGAGTCCCTGGACCCCGTCTTGGTTCATAGTGGGAGTGACCCTTGTTCCTCCATGTATAACCTGGGTTCTGATGGATCCCCGAGCCATGAAGCCCCTCCACTATTTCCCTTTCCCATGGGAATAATTTTTTGGGAATTGGATGGAATGGGGTAGAATGAATAAAAATACACTTCCCTTATTTGGATATTTTGCCATTAAATTGAATAAAGCTTTCACTCCCTTATTTGGCTTGGCTAATTAATGGAATGATTATTTTTT



gi|45396782|gb|AV773100.1|AV773100 AV773100 Lotus japonicus Pods (20-30 mm in length) Lotus japonicus cDNA clone MPD046b03_f 3',

TGACAGAAGAGAGTGAGCACA
||||||||||||| |||| ||
ACTGTCTTCTCTCTCTCGCGT

EST sequence (targeted fragment marked with yellow):
TCCCTCCTCTACCACAATTGATAACCAACTTTCAGCTGCTTCTGTGAGCCACTCGCTTCTCAGGACCAGTTCAGTTCAGGACTCCCCTTGCGCTCTCTCTCTTCTGTCATCATCATATTCATCACAGACTCACGCACCTGGAAATGGTTTGGATCAAATGGTGCAGCCTCACGCAATGTCAATGTCCCTTATGGAGCCATTAGGCCTGAGCCTGCATGATAATAACAGCAGCTTAGAGTCCATGGACCCAGTCCTGGTTCATAGTGGGAGTGACCATTGTTCCTCAATGTATAACATGGGTTCTGATGGATCCCAGAGCCATGAAGCCCCTCCACTATTTCCTTTTCCATGGGAATAATTTTTTGGGAATTGGATGGAATGGGGTAGAATGAATAAAAATACACTTCCATTATCTTGGATATTTTGCATCTACACTTGAATAAAGCTTTCACTCCCAGTACCTGGCTTGGCTCATTAATGAATGATTATTTTTTAT



gi|45353324|gb|AV770069.1|AV770069 AV770069 Lotus japonicus Pods (20-30 mm in length) Lotus japonicus cDNA clone MPD001d06_f 3',

TGACAGAAGAGAGTGAGCACA
|||||.||||| |||.||||
ACTGTTTTCTCGAACTTGTGT

EST sequence (targeted fragment marked with yellow):
GTTCATTTGTGGTGTGCATAAAAGCTTGATGAGTTCATTTGCATTACTGGCGGGAGGGAAGGCCTTCACCTTGTTTGAAATAATGCTTTAATGTGTTCAAGCTCTTTTGTCATCCATTTACTGGACCGGGGAATATTTCCTTACTATATTTAAGCTATCAAATGTCTTGGTTGTATAGGAAGGGCTCCTGGGGAAGTGTTTTGATAAGTGAGGACTTGGTTCCTTCGGATACAGATTCTAGGGAGATTAAGAGAGTATGGTGCGTAGTTCCCCAACTCTGGTGTAGGGTGGGAAGATTTTTGGGAATTTCAGTCCTTGTAATGTTATCACTCTTGTATTGAATGAATGCTAACTTTATATTTTAAAACTTTTGTTCCTT



gi|45403669|gb|AV780024.1|AV780024 AV780024 Lotus japonicus Pods (20-30 mm in length) Lotus japonicus cDNA clone MPDL069d01_f 3'

TGACAGAAGAGAGTGAGCACA
|||||.||||| |||.||||
ACTGTTTTCTCGAACTTGTGT

EST sequence (targeted fragment marked with yellow):
ATAGTCTAGCATGTCTTCTCTATGTAAGTAGACGAATACTATCATGCCACCATTCGAATCCGTTCATTTTGAGTTAACTAGACATCTTTAGGTCTGAGGTAAAGTAGTAAACTAATGCGTAATTGGTGTATGTTTATGAAACTTTCCTTGTTTGCACGGTTTGCTCTGAACAGTCTTCTTTTTTACATCATTGAGCAGGTTCATTTGTGGTGTGCATAAAAGCTTGATGAGTTCATTTGCATTACTGGCGGGAGGGAAGGCCTTCACATTGTTTGAAATAATGCTTTAATGTGTTCAAGCTCTTTTGTCATCCATTTACTGGACCGGGGAATATTTCCTTACTATATTTAAGCTATCAAATGTCTTGGTTGTATAGGAAGGGCTCCTGGGGAAGTGTTTTGATAAGTGAGGACTTGGTTCCTTCGGATACAGATTCTAGGGAGATTAAGAGAGTATGGTGCGTAGTTCCCAAACTCTGGTGTAGGGTGGAAAGATTTTTGGGAATTTCAGTCCCTTGTAATGTTATCACTCTTGTATTGTAATGAATGCTAACTTTATATTTTAAAACTTTTGTTCCT



gi|45398359|gb|AV774685.1|AV774685 AV774685 Lotus japonicus Pods (20-30 mm in length) Lotus japonicus cDNA clone MPD071e07_f 3',

TGACAGAAGAGAGTGAGCACA
|||||.||||| |||.||||
ACTGTTTTCTCGAACTTGTGT

EST sequence (targeted fragment marked with yellow):
TTTCTGCTGATATATCAAACCTTCCAGCATGGTCATTTGTGGTGTGCATAAACGCTTGATGAGTTCATTTGCATTACTGGCGGGAGGGAAGGCCTTCACCTTCTTTGAAATAATGCTTTAATGTGTTCAAGCTCTTTTGTCATCAATTTACTGGACCGGGGAATATTTCCTTACTATATTTAAGCTATCAAATGTCTCGGTTGTATAGGAAGGGCTCCTGGGGAAGTGTTTTGATAAGTGAGGACTTGGTTCCTTCGGATACAGATTCTAGGGAGATTAAGAGAGTATGGTGCGTAGTTCCCAAACTCTGGTGTAGGGTGGAAAGATTTTTGGGAATTTCAGTCCCCCCAATGTTATCACTCTTGTATTGAATGAATGCTAACTTTATATTTTAAAACTTTTGTTCCTGTCAT