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mature miRNA: TGAGCCGGATCAATATCACTC
species: Lotus japonicus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|45397386|gb|AV773706.1|AV773706 AV773706 Lotus japonicus Pods (20-30 mm in length) Lotus japonicus cDNA clone MPD055g02_f 3', 

TGAGCCGGATCAATATCACTC
 |||||||||||||||| |||
TCTCGGCCTAGTTATAG-GAG

EST sequence (targeted fragment marked with yellow):
ATTAGAAGTGATATGTGCAAATCCCATTTATACAATTTTAGAGGAGATGCATGAGACAAATGAAGAAATATTGATCAGGCTCCAAGCTGAGGATATTGATCCGGCTCTTGCTGGAAATATTGCACCCGTCTCTTATGAAGTATACATAGATATCATTCGCAGGTGCTTAAAGCTTGAAGCAAATGAGCGACCAACCATGGGCGAAGTGGAGATGCTACTTGAGCATGCTCTGACTTTACCACAGGAAGCCGAAGCCACAGATACTAGTGATGATTATCTCTGATTCTTCACTAGCATTATCAAACCATAACGATGAGAAGCGTCAATTTCAGTCTTGAGTATGTTAATTTGGTGAGTCTTGGATGGATTAAACATCATGCACTGATCGCTGATGGGGTTAATCTTCGTTTGTCTCAATTATACACAACTATAAACTTGCTCTTGTTTTTGTTCTGTTGGCTAAATTCATTAATCGTGT


gi|293529834|gb|FS352145.1|FS352145 FS352145 LjFL3 Lotus japonicus cDNA clone LjFL3-022-DG11.r0 3', mRNA sequence 

TGAGCCGGATCAATATCACTC
 |||||||||||||||| |||
TCTCGGCCTAGTTATAG-GAG

EST sequence (targeted fragment marked with yellow):
AAGGAAAACGCGTTGTTGGTACACTTGGTTACATGGCCCCCGAGAATATCAGGGATGGCATCTTGACAGATAAATGTGATGTTTATTCATTTGGCATTGTTCTATTAGAAGTGATATGTGCAAATCCAATTTATACAATTTTAGAGGAGATGCATGAGACAAATGAAGAAATATTGATCAGGCTCCAAGCTGAGGATATTGATCCGGCTCTTGCTGGAAATATTGCACCAGTGTGTTATGAAGTATACATAGATATCATTCGCAGGTGCTTAAAGCTTGAAGCAAATGAGCGACCAACAATGGGCGAAGTGGAGATGCTACTTGAGCATGCTCTGACTTTACAACAGGAAGCCGAAGCCACAGATACTAGTGATGATTATCTCTGATTCTTCACTAGCATTATCAAACCATAACGATGAGAAGCGTCAATTTCAGTCTTGAGTATGTTAATTTGGTGAGTCTTGGATGGATTAAACATCATGCACTGATCGCTGATGGGGTTAATCTTCGTTTGTCTCAATTATACACAACTATAAACTTGCTCTTGTTTTTGTTCTGTTGGCAAAATTCATTAATCGTGTATTTTGGTCTAAAAAAAAAAAAAAA


gi|223427943|gb|GO015881.1|GO015881 LJGAV56TO JCVI-LJ1 Lotus japonicus cDNA 3', mRNA sequence 

TGAGCCGGATCAATATCACTC
 ||||||||||||||| ||||
TCTCGGCCTAGTTATA-TGAG

EST sequence (targeted fragment marked with yellow):
GAGCATGTCACAGAAAAGCCAAAACCAATTCAAGTAAAGAAGTTTTCATCAACACAAAGCGGTACATACAATTACATGGCCCCGAATATATACGCAAAGGCACCGTGACGGATCAATACGATGTTTATTCATTTGGTATTGTTCTAATAGATGTGGTATCTCGAAAGCCAGTTGATGCAATTAGGAATGAGATGTTTGGTTTCCAGCAACCAACAAATGAAAAAAGAGAAATGTCTAATAATGGTTCAGAATCTTCTTCAGATGATCATATTACTATGATTGAGGTGCCTGCGCCTGAACCACCAAATGAAGAAGTAGAAATGTCTAATAATGGTTCAGAATCTTCTTCAGATGATCATATTACAATGATTGAGATGCCTGTGCCTGAGCCACCAAATGAAGAAGTAGAAATGACCAATAATGATTCAGAATTTTCAGATGATCATATTACTATGTCTTGGGGTTCTAAGATTAATATGTTAAGGCTTCGAGCAGAGTATATTGATCCGGCTCTGGTAGGAAATATTGCACCACAATGTTATGCAGCATACATCGATATCATTGACAGGTGCTTGAAGGATGAACCAAATGAACGACCAAACATGGGCGAAGTGGAATTGCTACTTGAGCATGCGCTGACTTTGCAACTGGAGGCAGATGCCACAGATACTAGTGATCATTGATTACCTCTAATTATTCACCAGCATTACCCAACCACAACAACCAGAGATGCCAATTTCAACTTAGAACATGTTGCCTTGTTGGTTTGGCTGTCTCATATCGGATCATGTTATTCTTTCTAGTCATCACTTGACTTTTTCCTGTGTTGTTAGCAAAATTGCTTAATCATGT