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mature miRNA: TTGACCGTTCATAGGAACCCT
species: Medicago truncatula
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|14881954|gb|BI272539.1|BI272539 NF023D09FL1F1077 Developing flower Medicago truncatula cDNA clone NF023D09FL 5', mRNA sequenc

TTGACCGTTCATAGGAACCCT
||||||||||||| .| ||||
AACTGGCAAGTATGTTAGGGA

EST sequence (targeted fragment marked with yellow):
GTTTCTCTCATTTTCATCCAGTATCGAATAAATTCGGATTATATAATCCGAAAACCGTGTGTAGGACCAAGTCAGTGTTTTTGGCCGGTGGTCTGCACCACATAAAACACGTTTTCAAATGTTTTTACCAGCATTAATGGTGGGTAAAACCCATTTGAAACATATTTTTTGACCCAACATTAAGTGTAGATACAATCTATGCAACCATTCTTCCTATAAATAGCCTCCAAACCTTCACCATTTCTCACCGTCATCTCACACTCTCTCCTCTCTCCTCCTCTTCCTTCTACAACAATGTGTTTTCACCGTTGGCCTTTCATAAAAAGATGGCTATACTTTGGAAATTCAGGGATTGTATGAACGGTCAATGAGGATGGCAACGCACTGCAAAGAAGTGTGGTTTGTCTGGGTGCAAGATTGACCATCAAGATCTCTTTCGCCCTAAAGAGGAAATAAATCTTGCCTCAGCCCCCATCACCATCTATTTATCCTCTCCCATTCCTCCCCAGCTTTATTGTAAATGTATTACTATAATAATAAAATGAAATTTTATGTGTAAAAAAAAAAAAAAAAAA



gi|157979187|gb|EX527459.1|EX527459 MTGland_A029_2007-06-18/MTGlandA029_A10_040_1 Medicago truncatula A17 glandular trichome Med

TTGACCGTTCATAGGAACCCT
|||||||||||||||||||||
AACTGGCAAGTATCCTTGGGA

EST sequence (targeted fragment marked with yellow):
TTAACAAATTNATGANCCCTGTTTGANGTAAAAAAAAATTGAATTTAGGNTCAAAAAANAAAAANAAGTCATTTATAAATTATTAAAATACACACTTCAATGGGACTTGAACACATCATAATANACTAACCATGTAACTGAAGCATTGTGAACAAATTGGAAATTATTCACTTTCAATGTATATAACCATTATTTTACGACTATTACTTTTCCCACCCTACAGCCTTCTCGCGCGCCCTATGATTTTGACCAAAATACCCTTTGTTCGGATTGTATAATCTGAATCAGATTGTTAGTGATTTCCAGAAACAGAAATCCGGACCAGATGTTATGTAATTTTTTGTGTCTTTCTTTGATGTCAAGGGTCTCTGTTGACCGTTATATGAACCACACACAAGCTGCAATGCATTGCAATTGTAGGGTTCCTATGAACGGTCAACAATGACCATGACACCNTCGACACCCCTAAAAAACTAAAAAAAACATTTGAAGAAAATGTAAAAAAAGNNNNGAAGTGAAGAATGTANGTATTTGTTGAAGATGGAAGCCTATTTATNNNCTAAAACNAGTTCT



gi|209567533|gb|GE350737.1|GE350737 MEUAS74TR JCVI-MT2 Medicago truncatula cDNA 3', mRNA sequence

TTGACCGTTCATAGGAACCCT
|.|||||||||| ||| ||||
AGCTGGCAAGTAACCT-GGGA

EST sequence (targeted fragment marked with yellow):
ATTGGAGTTATTCTGACCACTCTCCTTTGTGCAAGCCCAATCGGGCAAGTCGCGGAAGTGTTAAAAACTCAAGGAGCACAATTGATATTGCCAGTCCTTGCTTTACATGCTGCTGCATTTGCTATTGGTTATTGGATGTCAAGAATATCATTTGGTGAATCCACATCACGCACAATATCTATCGAGTGTGGGATGCAGAGCTCTGCACTTGGATTCTTGCTTGCTCAGAAGCACTTTACCAACCCCCTTGTAGCTGTTCCTTCTGCTGTCAGTGTTGTCTGCATGGCGCTTGGCGGGAGTGCTCTAGCTGTGTATTGGAGGAATAGTCCGATTCCCGCTGATGACAAAGACGACTTCAAGGAGTGAAAACCATGACATATTTTCTATCCACTGCATGCCCTATGAATTATTTTCCGCTCATTGTTTAGAAGACATTAATTTATGCATTAGTTGATAAGAAAGTTTTGTTTTATGAATTCATGTAGCCTTGCAATGTCAGAGTTTCGTAGAATTTAGGATAAGCTTGCAACAATTTAAACCAGTGTGAAGAGTGGCAACAAATAATATGCCCATGTAGCAACAAAAAGGGACAACCTATAGCTACTGAGCTCTTGCCATGGTAGGGTCCAATGAACGGTCGAATCCATTGTGGTTCTATTATAATTATTAGTCTTGTCTTGCATTGTGGTTCAACCACTTCAACTAGTGATATTCAGTTACAACAATTGTGTCAAGAAGACTCTATCAATGTATCAACTAATTCCTTCTTAGTAACTATGAAGTTATATTATATTATTATCTGAAGTTCTCGAAAAAAAAAAAAAAAAAA



gi|157980980|gb|EX529252.1|EX529252 MTGland_A051_2007-07-11/MTGlandA051_H11_041_1 Medicago truncatula A17 glandular trichome Med

TTGACCGTTCATAGGAACCCT
||||||.||||||..| ||||
AACTGGTAAGTATTTTAGGGA

EST sequence (targeted fragment marked with yellow):
ACGGCCGGGCCTCTCCTCTTCTTCCTTCTANNACCATCTTCTCCTCCTCCTTCTACAACCATGGTTTTCCATCACCGACCTTTCATAGAAACAGGCCTCTTTGAAAATTTTCAGGGATTTTATGAATGGTCAATGGGGCTAATGGCCATTGGTTTCTTCTTCCCGCCCTCCTATTTCCTCCTCCCCTGTCTCCCCTCCAACGGTGAAGCCACTGGTATGTCCTATCAGCCGGAGTCACTGGTATAACTTACCCCCGCCCCTCCCTTAGAAATAGTTTTAGGTTCTTATGTAAAAAGCTTCAACATTATTGTAATGAATTATTCAAATATCAATAAAATGAGTTGTTTTTATCTTTTTATTTCTGTTTTATATGTTTATTTGAATTTATATTACGAATTTCAAAGCTTAAAATTGTAAAAGTTTTGGAAAAAGACCATTCCGGAATTTAAAATCCGGATAAATCTGGAAAACATTAACAATTCATTCCGGATAATTGTTGTGTGAAGGCAATTTCATAGTTGTGCCTTATCGTTTTTCTATGTCTGTTTGATATAGATTTTAGGCCAATTTTGTTGTTTGAGTTTTAGTCCATATTTTGTATTTTAAGTTTTAATATGTAGTTTAAATTTAATTTTTCTATTGTAATATCCCAACCGTATCATATCTTACATTTTAAAATATTCTCATATCAGCATANNNNNGTCGTATTATATCTGTATTGTATCATATAGTTGGGCTTCATATTATCTTATCTTATTGATTGAAATTTAATTGACATCCACTTAANTATCCTACATAAATTTTTANGGTCTATATACATTTTACCCAATAAAAAAAATAAANAAATCACTATTCACCACCAAAATTGNNNGGTCGGACATTTACCCCANTNGTGAATAATCANNAAATACTCTATTTGNATTACGTCGANCAAGTTTNNCNTTGATAACNTGTATCGTATCTTTACGATCNAATTTANTATANTACTTACTAGCATATNNTCCCCNAAATTAAANA