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mature miRNA: TGCATTTGCACCTGCGCCTTC
species: Mimulus guttatus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|238433249|gb|GR167292.1|GR167292 CBPF12243.g1 CBPF Mimulus guttatus IM62 roots, seedlings, fruits, callus (H) Mimulus guttatu

TGCATTTGCACCTGCGCCTTC
|||||.|||||||||. ||||
ACGTAGACGTGGACGTAGAAG

EST sequence (targeted fragment marked with yellow):
ATGGATATATAACTTGTGGTTCAGAGACAAACGAGGTGTACGCCTATCACAAATCTCTGCCCATGCCAATTACTGCCCACAAATTCGGCTCGATCGATCCTGTAACTGGAAAAGATACTGAAGATGACAACGGACAATTTGTTTCAAGTGTTTGCTTCAGACGTAAATCGAATATGGTGGTCGCTGCCAATTCATCGGGGTGTATCAAACTACTCCAGCTCGTTTAGAAACACTCTTCTCTCATATGTACAGGAGTAACACGATTAGTATTTTTTTGCTGGGTTGTGGAAAAAAAGTACGTTCTTCTGAGTTTACTGCTGAAAGACGAACGAGAAGATGCAGGTGCAGATGCAGGAGCTTTATGTGATTTATCGGTTTCTACAAAAGTTGGTAAATAGCTTCCTTCTGTTAGCCCGGCCACCACCAGTTTATGCGAGTGCTGCAATTGAATTTTATTGTTCATGCAATAATGTTTTTGTGTGATTGAGGTGGCAATTTATTTTAGCAGCATTCTTTGTGTATCCAATGTGTATAACTTATGTGCAAGGTTAACATATACCCAGATTTGTGGATTAGCTGTTTGTATTTGCTCTCTTAAAATTCTTGTTCCATGTATACAAGTGGAAGAAAACCTTATAGCAATTGTTCTTTTTCTT