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mature miRNA: ATACTCCTTCCGTCCCAATTT
species: Nicotiana benthamiana
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|150429434|gb|ES888027.1|ES888027 NBT062_2006-12-08_1/NBT062_B04_015_1 Nicotiana benthamiana trichomes Nicotiana benthamiana c

ATACTCCTTCCGTCCCAATTT
||| |||||||.| | |||||
TATAAGGAAGGTAAGTTTAAA

EST sequence (targeted fragment marked with yellow):
GGCCGGGNANTGNNTTGAAGATGTATATTTGTTGTGTACATTGCCACTAAGCTCTATAAGGACATGTTGTGGTTTATGGAGTATGGACCTGCATGCCTTGCATTGTCAAATTTGAATGGAAGGAATATATTACATGTTGCATGTTATGGTGGTTCTTTTCTGTACAGGCATGAAGCGATACTGTTTTANCATTATATAAACAACAACAACAACAACTACCCAGTATATTCCCAGCAGGTGGGGTCTGGGGAGGGTAATATGTANACNANGCTTACCCCTGCCC



gi|39868188|gb|CK289568.1|CK289568 EST752290 Nicotiana benthamiana mixed tissue cDNA library, normalized, full-length Nicotiana

ATACTCCTTCCGTCCCAATTT
||||||.||||| ||||||
AATGAGGGAGGCAAAGTTAAA

EST sequence (targeted fragment marked with yellow):
GTCCTTTTGTTTTCTTGTTTAATTCAACTCTTTGCTTAACCACAACACTCAACTTTCATAAACTAAAGGCACAATTCTTTTTCTAAAAACGCGATGTTCAGGCAAGCAACACTTCTAAACTAATCTATCAAATACCTACAACTTCCGTACCAGCAAACGAATTCGGTAACTTTGCCAATCAAGACTTGGATAGATGGAACAGTTCACCTAGTAACGCTGGCTCAAGGTCGTCATTCCATATGTTGACTAAGCCAGCCCCTCAGTGGCAACTAAAGACACAATATTTACGGCATACTTCTTAAGTAAATCTCAAATTTCAAGAATCAAAAGTAATACTCCCTCATTTTCAATTAACGTGAACCTATTTCCTTTTTAGTCGGTGCAAAAAAGAATGACCTCTTTCCTTATTTGAAAAATAATTTACCTTTATGCAATGATTTATAACCACACAATATATATCTACCTTATTTTACGCCACATGTTCAAAAGTGTCCTCTCTTTTTTTAAACTTCTTGCCAGTCAAATGGGTTCACATAAATTGAAACGGAGGGAGTAAGTTTTATAGTGTAATAGAGTAATCCTCTGTTCCAATTTTGTGCAATATTCTTTCTTTTTTAGTTTGTTCCAGAAAAAACTATATTTTTCATATTTAGAAACAATTTTAAATTTAAAGTTCTCATTCTCTCCCCTTAATGAGATGATCTATAGCCAAACAAGTATCGCTCGCTTGTTTTAGATCAAAATTTCAAAAAATCTTTCTTTCTTTCTTTCTTAAACTCTATGCCAGTCAAACAAATTGGGACATAAGGAGTGTATTAACTAAAAGTAAGT



gi|227564692|gb|GO601651.1|GO601651 NBERO1CH_T3_016_H02_01MAY2006_002 NBERO1CH Nicotiana benthamiana cDNA, mRNA sequence

ATACTCCTTCCGTCCCAATTT
||||||||||.| |||||||
GATGAGGAAGGTAAGGTTAAA

EST sequence (targeted fragment marked with yellow):
TAAATACAGATCTGAGCCTCAGTTCTCATCCCATAATCTTACTTTTGGTGTAATTGGCATGGAGGAAACTCTTTCGGTCAACCAAGACTGCTGGATTCAGAGAGTTTTGGAAAGTTCTTTGAGTGGTTTCCCCTCTTTGGCACTCTCAGCAGTCAATGATCGCTCAAAGTTTCCCCTTTTCACATGACTCTTTAGCAATTAAGTTCAAATGAGGCAGTGAAGATTTTTATTCTTTACTCATCAGATGGTTGAACTTTGCTATGTCTTGGCGTGGCAGCTTAAGGCTTCTTAAGCTTGCAGCCATGGCAGGGTTTGGCTTATGTTTGAGAAAAAGCTAATCTGAGCCTCCAGTGGCTTCTCATATGAAAATAAAGAGACTCAAACAGCCCTTTAAATGGTGGGATCTTTACCGAAATAGCTGGCCAGATTCACTATTATTCCCCCGTTCCACTTTATGTAAACCTATTTTCTTTTTGATCCGGTCCAAAAAGAATGATCTCTTTCTAAATTTGGTAACAATTTAGATTAAACTTACAGTTCTACCTTTAACGAGTAATTTTTATAATCACACAAATACTCTGATCCCCTAGGTTCACATAAATTGGAATGGAAGGAGTAGCTTCTTCCAACCAGTATACATGGATTATACACTACTTGTACAGTCAAACTACTCTGTAACAACCATCCTCTCTAACAACGCTTCACTGTAACGGCCAAGTTTTCTTTGGAACCAATTTCAGTGTTGTCAAAGGCGCGGTTAAAGCG